Detailed information NTG1126109

Bioinformatically predicted

Overview

• Name: comEC/celB
• Type: Machinery gene
• Locus tag: E0F32_RS04505
• Genome accession: NZ_LR216050
• Coordinates: 827595..829835 (+)
• Length: 746 a.a.
• NCBI ID: WP_130885934.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 822595..834835

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E0F32_RS04460 (SAMEA3431333_00834)	-	822745..823713 (+)	969	WP_000658183.1	PhoH family protein	-
E0F32_RS04470	-	823906..824407 (+)	502	Protein_844	GNAT family N-acetyltransferase	-
E0F32_RS04475	-	824410..824735 (+)	326	Protein_845	TfoX/Sxy family protein	-
E0F32_RS11920	ald	825036..826147 (-)	1112	Protein_846	alanine dehydrogenase	-
E0F32_RS04495 (SAMEA3431333_00841)	-	826324..826893 (+)	570	WP_000443774.1	GNAT family N-acetyltransferase	-
E0F32_RS04500 (SAMEA3431333_00842)	comEA/celA/cilE	826961..827611 (+)	651	WP_024477778.1	ComEA family DNA-binding protein	Machinery gene
E0F32_RS04505 (SAMEA3431333_00843)	comEC/celB	827595..829835 (+)	2241	WP_130885934.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
E0F32_RS04510	-	830014..830202 (+)	189	WP_001808514.1	hypothetical protein	-
E0F32_RS04515 (SAMEA3431333_00844)	-	830235..830822 (+)	588	WP_000939895.1	ATP-binding cassette domain-containing protein	-
E0F32_RS04520 (SAMEA3431333_00845)	-	830826..832010 (+)	1185	WP_000655960.1	ABC transporter permease	-
E0F32_RS04525 (SAMEA3431333_00846)	infC	832321..832851 (+)	531	WP_000848180.1	translation initiation factor IF-3	-
E0F32_RS04530 (SAMEA3431333_00847)	rpmI	832884..833084 (+)	201	WP_001125943.1	50S ribosomal protein L35	-
E0F32_RS04535 (SAMEA3431333_00848)	rplT	833136..833495 (+)	360	WP_000124836.1	50S ribosomal protein L20	-
E0F32_RS04540 (SAMEA3431333_00849)	-	833553..833933 (+)	381	WP_000157154.1	VOC family protein	-

Sequence

Protein

• Length: 746 a.a.
• Molecular weight: 84373.77 Da
• Isoelectric Point: 9.4491

>NTDB_id=1126109 E0F32_RS04505 WP_130885934.1 827595..829835(+) (comEC/celB) [Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505]
MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCLFIQFPWKSAGKVLVICGIFGFWFVFQNWQQSQASQN
LADSVEGVRILPDTIKVNGDSLSFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLLGHLDTDFDEMNELYSSLGIIHL
FALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPFSLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIV
MPNFFLTAGGVLSCAYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDLTFLPLLSI
LFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLISLALVYDLRKNIKKLTVLCLLITGLFLLTK
HPLENEITMLDVGQGESIFLRDVTGKTILIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDK
ENVGDLSEVTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMTVGENLPIFGSQLEVLSPRKMGDGGHDDTLVLYGKF
LDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSSSPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGIN
SKVYRTDQQGAIRFKGLDSWKIESVR

Nucleotide

• Length: 2241 bp

>NTDB_id=1126109 E0F32_RS04505 WP_130885934.1 827595..829835(+) (comEC/celB) [Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505]
ATGTTACAGTGGATTAAGAATTTCTCTATTCCCCTAATTTACCTGAGTTTTCTATTACTTTGGCTTTATTACGCTATTTT
CTCAGCATCTTATCTTGCTTTGTTGGGCTTTGTTTTTCTGCTAGTCTGTCTCTTTATCCAATTTCCGTGGAAATCTGCTG
GTAAAGTTCTAGTGATTTGTGGAATCTTTGGATTTTGGTTTGTTTTTCAAAATTGGCAACAGAGTCAAGCGAGTCAAAAT
CTGGCGGATTCTGTTGAAGGGGTACGGATTTTGCCTGATACTATTAAGGTTAATGGTGATAGTCTATCCTTTCGTGGCAA
GTCTAACGGTCGTGCTTTCCAAGTCTATTATAAACTCCAGTCCGAGGAGGAGAAAGAAGCCTTTCAAGCTTTAACCGACC
TGCATGAGATAGGACTAGAAGGGAAGCTTTCGGAGCCAGAAGGGCAGAGAAATTTTGGTGGCTTTAATTACCAAGCCTAT
CTGAAGACTCAGGGAATTTACCAGACTCTCAATATCAAAACAATCCAGTCACTTCAAAAGATTGGCAGTTGGGATATAGG
AGAAAACTTGTCCAGTTTACGTCGAAAGGCTGTGGTTTGGATTAAGACGCACTTTCCAGACCCTATGCGCAATTACATGA
CAGGACTCTTGCTGGGACATCTGGACACCGACTTTGATGAGATGAATGAGCTTTATTCCAGTCTAGGAATTATCCACCTC
TTTGCCCTATCTGGTATGCAGGTAGGTTTTTTCATGAATGGATTTAAGAAACTTCTCTTGCGATTGGGCTTGACCCAAGA
AAAGTTGAAATGGCTGACTTATCCCTTTTCCCTTATCTATGCGGGACTAACTGGATTTTCAGCATCGGTTATTCGCAGTC
TCTTGCAAAAGCTACTGGCTCAACATGGGGTTAAGGGCTTGGATAATTTTGCCTTGACGGTGCTTGTCCTCTTTATTGTC
ATGCCAAACTTTTTCTTGACAGCAGGAGGAGTCTTGTCCTGCGCTTATGCTTTTATCCTGACCATGACCAGCAAAGAAGG
GGAGGGGCTCAAGGCTGTTACTAGTGAAAGTCTAGTCATCTCCTTGGGCATATTGCCCATTCTATCCTTCTATTTTGCGG
AATTTCAACCTTGGTCTATCCTTTTGACCTTTGTCTTTTCCTTTCTATTTGACTTGACCTTCTTACCGCTCTTGTCTATT
TTATTTGTCCTTTCCTTTCTCTATCCAGTCATTCAGCTGAACTTTATTTTTGAATGGTTGGAGGGCATTATTCGCTTGGT
GTCACAGGTGACAAGTAGACCTCTGGTCTTTGGACAACCCAATGCATGGTTTTTAATCCTATTGTTAATTTCCTTGGCTT
TGGTCTATGATTTGAGAAAAAACATTAAAAAGCTAACGGTATTGTGCTTATTGATTACAGGGCTCTTTCTCCTGACCAAG
CATCCACTGGAAAATGAAATCACCATGCTGGATGTGGGGCAAGGAGAAAGTATTTTCCTACGGGATGTAACTGGGAAAAC
CATTCTCATAGATGTAGGTGGTAAGGCAGAATCTTATAAGAAAATCGAAAAATGGCAAGAAAAGATGACGACCAGCAATG
CCCAGCGAACCTTGATTCCCTATCTCAAAAGTCGAGGAGTAGCTAAGATTGACCAGCTAATTTTGACTAACACGGACAAG
GAGAATGTTGGAGATTTGTCAGAGGTGACCAAGGCTTTCCATGTAGGGGAGATTCTAGTATCAAAAGACAGTCTGAAACA
GAAGGAATTTGTGGCAGAACTACAGGCGACTCAAACAAAGGTGCGTAGTATGACAGTAGGGGAGAACTTGCCCATTTTTG
GAAGTCAGTTAGAAGTTCTATCTCCAAGGAAAATGGGAGATGGAGGACACGATGATACCCTAGTTCTGTATGGGAAATTC
TTGGATAAGCAATTTCTCTTCACGGGAAATTTGGAGGAGAAAGGAGAGAAGGACTTGCTGAAGCACTATCCAGACTTGAA
AGTAAATGTTTTGAAAGCTAGCCAACATGGCAATAAAAAATCATCAAGTCCAGCCTTTCTAGAAAAACTCAAACCAGAGC
TTACTCTTATCTCAGTTGGAAAGAGCAATCGAATGAAACTCCCCCATCAGGAAACATTGACACGACTGGAAGGTATCAAT
AGCAAAGTTTATCGAACTGACCAGCAAGGAGCTATACGTTTTAAGGGGTTGGATAGTTGGAAAATCGAAAGTGTTCGATA
G

Domains

Predicted by InterproScan.

• Domain of unknown function (DUF4131) (DUF4131) (31-176)

• Competence protein (Competence) (216-458)

• Metallo-beta-lactamase superfamily (Lactamase_B) (492-693)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC/celB	Streptococcus pneumoniae TIGR4	97.855	100	0.979
comEC/celB	Streptococcus pneumoniae D39	97.587	100	0.976
comEC/celB	Streptococcus pneumoniae R6	97.587	100	0.976
comEC/celB	Streptococcus pneumoniae Rx1	97.587	100	0.976
comEC/celB	Streptococcus mitis SK321	91.823	100	0.918
comEC/celB	Streptococcus mitis NCTC 12261	91.678	99.866	0.916
comEC	Lactococcus lactis subsp. cremoris KW2	44.13	99.33	0.438