Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EL309_RS04220 Genome accession   NZ_LR134533
Coordinates   861683..863362 (-) Length   559 a.a.
NCBI ID   WP_004282574.1    Uniprot ID   -
Organism   Neisseria weaveri strain NCTC12742     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 856683..868362
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL309_RS04195 (NCTC12742_00825) yacG 857972..858154 (-) 183 WP_081463174.1 DNA gyrase inhibitor YacG -
  EL309_RS04200 (NCTC12742_00826) zapD 858160..858933 (-) 774 WP_004282578.1 cell division protein ZapD -
  EL309_RS04205 (NCTC12742_00827) coaE 858930..859553 (-) 624 WP_004282577.1 dephospho-CoA kinase -
  EL309_RS04210 (NCTC12742_00828) pilD 859555..860415 (-) 861 WP_004285616.1 A24 family peptidase Machinery gene
  EL309_RS04215 (NCTC12742_00829) pilG 860418..861659 (-) 1242 WP_004282575.1 type II secretion system F family protein Machinery gene
  EL309_RS04220 (NCTC12742_00830) pilF 861683..863362 (-) 1680 WP_004282574.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL309_RS04225 (NCTC12742_00831) comE 863589..863894 (-) 306 WP_064130790.1 helix-hairpin-helix domain-containing protein Machinery gene

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62286.84 Da        Isoelectric Point: 6.7673

>NTDB_id=1124114 EL309_RS04220 WP_004282574.1 861683..863362(-) (pilF) [Neisseria weaveri strain NCTC12742]
MSVGLLRVLVQSQILDESKVAKYQNAAQNHKNVISMLFEDGITTPKTLSELLARVFSYPLLDLRHYPKINIVNDILTEEQ
MLQNRCVPIVKKGRKVYLAVSDPTQIQNFQKIAFSADVSIDLLIVRDDQLSALLEWISQRSTTILKEISKEQESVSPSQT
LYIDNEEAEDGPIARFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RIPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNTDAGTLNINQLGLETFQKEILLEAIQRPYGMVLVTGPTGS
GKTVSLYTCLNILNTENVNIATAEDPAEINLPGINQVNINEKQGLTFAAALKAFLRQDPDVIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSVIMAQRLLRRLCPHCKAEMERPPVPALKKVGFTDNDLASDWKL
YRSVGCDSCRGKGYKGRVGVYEVMPISENMQRVIMENGSEVDITNIAYQEGMVDLRRSGLLKVMQGITSLEEVLANTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=1124114 EL309_RS04220 WP_004282574.1 861683..863362(-) (pilF) [Neisseria weaveri strain NCTC12742]
ATGAGCGTTGGTTTGTTGAGAGTTTTGGTTCAGAGCCAGATCTTGGATGAAAGTAAAGTTGCCAAGTATCAGAATGCTGC
TCAGAATCATAAGAATGTTATTTCCATGTTGTTTGAAGATGGGATTACAACGCCGAAAACACTGAGCGAGTTATTAGCTA
GAGTATTTAGTTATCCTTTGTTGGACTTACGTCATTATCCGAAGATTAATATTGTTAATGACATCTTAACAGAAGAACAA
ATGCTCCAAAATAGATGTGTACCTATTGTTAAAAAGGGTAGAAAAGTCTATTTAGCTGTTTCTGATCCCACTCAAATTCA
GAATTTTCAAAAAATTGCTTTTTCAGCGGATGTTTCGATTGATTTGCTGATTGTACGTGATGATCAGTTAAGCGCATTAT
TGGAATGGATTAGTCAGCGTTCTACAACAATTTTAAAGGAAATCAGTAAAGAGCAAGAATCTGTTTCACCGTCTCAAACA
TTATATATAGATAATGAAGAGGCTGAAGATGGGCCTATAGCAAGGTTTATTCATAAAACGCTTTCAGATGCTTTAAATGC
AGGTGCATCAGATATTCATTTTGAATTTTATGAACAAATGGCCAGGGTACGTTTTAGGGTTGATGGGCAATTGCGTGAAG
TGGTTCAGCCTCCTGTTGCAGTGCGAGGACAATTGGCTTCACGTATTAAAGTGATGGCACGCTTGGATATTTCTGAAAAA
AGAATTCCCCAAGATGGTCGTATTCAAATCGCTTTTCACAAGCATGGGAGACCTATTGACTTCCGTGTTAGTACATTACC
CACTTTATTTGGTGAAAAGGTGGTAATGCGTATTTTAAATACAGATGCAGGAACGCTTAATATTAATCAACTGGGTTTGG
AGACATTTCAAAAAGAGATTTTATTAGAAGCTATTCAGCGTCCGTATGGTATGGTGTTGGTTACTGGGCCTACAGGTTCA
GGTAAAACAGTTTCTTTATATACTTGTTTAAATATTTTAAATACAGAAAATGTTAATATTGCAACGGCTGAGGATCCTGC
AGAGATTAATCTGCCAGGAATTAATCAAGTTAACATTAATGAAAAGCAAGGACTTACTTTTGCGGCTGCGTTGAAAGCAT
TTTTACGTCAGGATCCTGATGTGATTATGGTGGGTGAGATACGAGATTTGGAAACTGCAGATATTGCCATTAAAGCAGCT
CAGACTGGCCATATGGTTTTTTCTACCTTGCACACGAATAATGCTCCGGCAACTTTGTCCCGTATGTTAAATATGGGTGT
AGCGCCGTTTAACATCGCTAGTTCTGTTAGCGTGATTATGGCACAGCGTTTGCTTAGAAGGTTATGTCCGCATTGTAAGG
CTGAAATGGAACGACCTCCTGTGCCTGCATTGAAAAAGGTGGGATTCACCGACAATGATTTGGCTTCCGATTGGAAGCTA
TATAGGTCTGTGGGATGTGACTCTTGTAGAGGTAAAGGCTATAAAGGTCGGGTTGGTGTGTATGAAGTGATGCCTATAAG
CGAAAATATGCAACGTGTGATTATGGAAAATGGTAGTGAGGTTGATATAACGAATATTGCCTACCAGGAAGGCATGGTTG
ATTTACGTAGATCAGGATTGCTAAAAGTAATGCAGGGTATTACTTCTTTAGAAGAAGTTTTAGCCAATACTAATGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-138)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

75.313

100

0.753
  pilB Acinetobacter baumannii D1279779

49.64

99.463

0.494
  pilB Acinetobacter baylyi ADP1

49.101

99.463

0.488
  pilB Legionella pneumophila strain ERS1305867

46.429

100

0.465
  pilB Vibrio cholerae strain A1552

45.196

100

0.454
  pilB Vibrio parahaemolyticus RIMD 2210633

44.841

95.349

0.428
  pilB Vibrio campbellii strain DS40M4

43.833

94.275

0.413

Multiple sequence alignment