Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EL300_RS06170 Genome accession   NZ_LR134532
Coordinates   1315175..1315609 (-) Length   144 a.a.
NCBI ID   WP_005741096.1    Uniprot ID   -
Organism   Pasteurella multocida strain NCTC10323     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1310175..1320609
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL300_RS06140 (NCTC10323_01252) - 1310742..1311011 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  EL300_RS06145 (NCTC10323_01253) yacG 1311011..1311214 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  EL300_RS06150 (NCTC10323_01254) coaE 1311225..1311845 (-) 621 WP_005751078.1 dephospho-CoA kinase -
  EL300_RS06155 (NCTC10323_01255) - 1311860..1312582 (-) 723 WP_005727302.1 prepilin peptidase -
  EL300_RS06160 (NCTC10323_01256) - 1312582..1313793 (-) 1212 WP_005751077.1 type II secretion system F family protein -
  EL300_RS06165 (NCTC10323_01257) pilB 1313793..1315175 (-) 1383 WP_005751075.1 GspE/PulE family protein Machinery gene
  EL300_RS06170 (NCTC10323_01258) pilA 1315175..1315609 (-) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  EL300_RS06175 (NCTC10323_01259) ampD 1315769..1316335 (+) 567 WP_005751074.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EL300_RS06180 (NCTC10323_01260) rppH 1316921..1317517 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  EL300_RS06185 (NCTC10323_01261) - 1317520..1318314 (+) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  EL300_RS06190 (NCTC10323_01262) lgt 1318324..1319127 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  EL300_RS06195 (NCTC10323_01263) - 1319133..1319984 (+) 852 WP_005751072.1 thymidylate synthase -
  EL300_RS06200 (NCTC10323_01264) tadA 1319999..1320499 (+) 501 WP_005751071.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15058.48 Da        Isoelectric Point: 9.1281

>NTDB_id=1124089 EL300_RS06170 WP_005741096.1 1315175..1315609(-) (pilA) [Pasteurella multocida strain NCTC10323]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1124089 EL300_RS06170 WP_005741096.1 1315175..1315609(-) (pilA) [Pasteurella multocida strain NCTC10323]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG
CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCTGCTCAAGGTGTTTCTTGGACAGTGAATTGTGGTGCTC
AATCTGACATTTTTCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

52.349

100

0.542
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.806

100

0.368
  pilA Vibrio cholerae strain A1552

36.806

100

0.368
  pilA Vibrio cholerae C6706

36.806

100

0.368

Multiple sequence alignment