Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EL300_RS06165 Genome accession   NZ_LR134532
Coordinates   1313793..1315175 (-) Length   460 a.a.
NCBI ID   WP_005751075.1    Uniprot ID   -
Organism   Pasteurella multocida strain NCTC10323     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1308793..1320175
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL300_RS06135 (NCTC10323_01250) - 1308920..1310293 (-) 1374 WP_005727312.1 alanine/glycine:cation symporter family protein -
  EL300_RS06140 (NCTC10323_01252) - 1310742..1311011 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  EL300_RS06145 (NCTC10323_01253) yacG 1311011..1311214 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  EL300_RS06150 (NCTC10323_01254) coaE 1311225..1311845 (-) 621 WP_005751078.1 dephospho-CoA kinase -
  EL300_RS06155 (NCTC10323_01255) - 1311860..1312582 (-) 723 WP_005727302.1 prepilin peptidase -
  EL300_RS06160 (NCTC10323_01256) - 1312582..1313793 (-) 1212 WP_005751077.1 type II secretion system F family protein -
  EL300_RS06165 (NCTC10323_01257) pilB 1313793..1315175 (-) 1383 WP_005751075.1 GspE/PulE family protein Machinery gene
  EL300_RS06170 (NCTC10323_01258) pilA 1315175..1315609 (-) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  EL300_RS06175 (NCTC10323_01259) ampD 1315769..1316335 (+) 567 WP_005751074.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EL300_RS06180 (NCTC10323_01260) rppH 1316921..1317517 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  EL300_RS06185 (NCTC10323_01261) - 1317520..1318314 (+) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  EL300_RS06190 (NCTC10323_01262) lgt 1318324..1319127 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  EL300_RS06195 (NCTC10323_01263) - 1319133..1319984 (+) 852 WP_005751072.1 thymidylate synthase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 52017.66 Da        Isoelectric Point: 6.5002

>NTDB_id=1124088 EL300_RS06165 WP_005751075.1 1313793..1315175(-) (pilB) [Pasteurella multocida strain NCTC10323]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLST
NELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQV
QPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGMT
ADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRTF
LRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCDCRVE
TQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=1124088 EL300_RS06165 WP_005751075.1 1313793..1315175(-) (pilB) [Pasteurella multocida strain NCTC10323]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGCGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACGCTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGTGTGG
ATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCAACA
AATGAACTTAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAACATACCCACGGTTTATCAAGT
CGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTACAGC
TACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAAGTC
CAGCCTTCCTTTTCAGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGAAAC
ACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGCCGA
CGCATTTAGGCGAAAAAGCGGTATTACGTTTACAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATGACC
GCAGACCAACAACACAGGTTCCAACACGCGTTAAGTCAGCCTCAAGGACTGATTTTAGTAACGGGACCAACAGGGAGTGG
AAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGATTG
AAATTGCGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACTTTT
TTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGCTCA
AACAGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGGTGC
CTTTGCATGAAATTGAACAAAGCCTCTTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTGATTGCCGTGTAGAG
ACACAATCGGCGTGTCGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAACAGG
GTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATGAAG
TACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(127-393)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(18-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

67.991

98.478

0.67
  pilB Haemophilus influenzae Rd KW20

67.329

98.478

0.663
  pilB Glaesserella parasuis strain SC1401

58.811

98.696

0.58
  pilB Acinetobacter baumannii D1279779

40.369

100

0.428
  pilB Vibrio parahaemolyticus RIMD 2210633

39.059

100

0.415
  pilB Vibrio campbellii strain DS40M4

39.419

100

0.413
  pilB Acinetobacter baylyi ADP1

37.876

100

0.411
  pilB Vibrio cholerae strain A1552

38.272

100

0.404
  pilB Legionella pneumophila strain ERS1305867

37.243

100

0.393

Multiple sequence alignment