Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EL323_RS09850 Genome accession   NZ_LR134526
Coordinates   1679956..1681632 (-) Length   558 a.a.
NCBI ID   WP_080541848.1    Uniprot ID   -
Organism   Neisseria meningitidis strain NCTC10026     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1674956..1686632
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL323_RS09830 (NCTC10026_01621) pilG 1676869..1678101 (+) 1233 WP_002216405.1 type 4 pilus assembly protein PilG Machinery gene
  EL323_RS09835 (NCTC10026_01622) pilD 1678175..1679035 (+) 861 WP_002221969.1 prepilin peptidase Machinery gene
  EL323_RS09840 (NCTC10026_01623) coaE 1679037..1679669 (+) 633 WP_002243235.1 dephospho-CoA kinase -
  EL323_RS09845 (NCTC10026_01624) - 1679662..1679871 (+) 210 WP_002212333.1 DNA gyrase inhibitor YacG -
  EL323_RS09850 (NCTC10026_01625) pilF 1679956..1681632 (-) 1677 WP_080541848.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL323_RS09870 (NCTC10026_01627) - 1682123..1682569 (-) 447 WP_014581766.1 DUF441 domain-containing protein -
  EL323_RS09875 (NCTC10026_01628) ispB 1682573..1683547 (-) 975 WP_002235439.1 octaprenyl diphosphate synthase -
  EL323_RS09885 (NCTC10026_01629) rplU 1683771..1684079 (+) 309 WP_002216394.1 50S ribosomal protein L21 -
  EL323_RS09890 (NCTC10026_01630) rpmA 1684104..1684376 (+) 273 WP_002212328.1 50S ribosomal protein L27 -
  EL323_RS09895 (NCTC10026_01631) ubiM 1684765..1685949 (+) 1185 WP_080541849.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -
  EL323_RS09905 (NCTC10026_01632) rpmG 1686220..1686375 (-) 156 WP_002212306.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62007.13 Da        Isoelectric Point: 5.3037

>NTDB_id=1123986 EL323_RS09850 WP_080541848.1 1679956..1681632(-) (pilF) [Neisseria meningitidis strain NCTC10026]
MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVISPKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQ
MVEFHCVPVFRRGDKVFFAVSDPTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEVQEEEESHTL
YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASRIKVMSRLDISEKR
IPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVGFTDEDLAKDWKLY
RAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=1123986 EL323_RS09850 WP_080541848.1 1679956..1681632(-) (pilF) [Neisseria meningitidis strain NCTC10026]
ATGAGCGTAGGTTTGCTGAGGATTCTGGTTCAAAACCAGGTGGTTACTGTTGAGCAGGCCGAGCATTACTACAATGAGTC
GCAGGCGGGTAAGGAAGTGTTGCCGATGCTGTTTTCAGACGGCGTCATTTCGCCCAAGTCGCTTGCGGCATTGATTGCGA
GGGTGTTCAGTTATTCGATTCTTGATTTGCGTCATTATCCGCGCCACAGGGTGCTGATGGGGGTGTTGACGGAGGAGCAG
ATGGTGGAGTTCCACTGTGTGCCGGTTTTCCGTCGGGGCGACAAAGTATTTTTTGCGGTTTCCGATCCGACACAGATGCC
GCAAATTCAGAAAACCGTTTCTGCCGCAGGGATTGAGGTTGAGTTGGTCATTGTCGAGGATGACCAGTTGGCGGGTTTGC
TCGATTGGGTGGGTTCGCGTTCGACATCGCTGCTTCAGGAGCTTGGGGAGGTGCAGGAGGAAGAGGAAAGCCACACCCTG
TATATCGACAACGAGGAGGCAGAAGATGGCCCTGTTCCGAGGTTTATCCATAAGACTTTGTCGGATGCCTTGCGCAGCGG
GGCATCGGACATCCATTTCGAGTTTTACGAACACAATGCCCGTATCCGTTTCCGTGTGGACGGGCAGCTCCGCGAGGTGG
TTCAGCCGCCCATTGCGGTAAGGGGGCAGCTTGCTTCCCGGATTAAGGTAATGTCGCGTTTGGACATTTCCGAAAAACGG
ATACCGCAGGACGGCAGGATGCAGCTGACCTTTCAAAAGGGCGGCAAGCCTGTCGATTTCCGTGTCAGCACATTGCCGAC
GCTGTTTGGCGAAAAGGTCGTGATGCGGATTTTGAATTCCGATGCCGCGTCTTTGAACATCGACCAGCTCGGTTTTGAGC
CGTTTCAGAAAAAATTGTTGTTGGAAGCGATTCACCGTCCCTACGGGATGGTGCTGGTAACCGGTCCGACGGGTTCGGGT
AAGACGGTGTCGCTCTATACCTGTTTGAATATTTTGAATACGGAGTCGGTAAATATTGCAACGGCGGAAGACCCTGCCGA
GATTAACCTGCCGGGCATCAATCAGGTTAACGTCAATGATAAGCAGGGCCTGACTTTTGCCGCTGCTTTGAAGTCTTTCC
TGCGTCAGGACCCGGACATCATTATGGTCGGTGAGATTCGTGATTTGGAAACTGCCGATATTGCGATTAAGGCGGCACAA
ACAGGGCATATGGTGTTTTCCACCCTGCACACCAATAATGCGCCGGCGACGTTGTCGCGTATGCTGAATATGGGTGTCGC
GCCGTTTAATATTGCCAGTTCGGTCAGCCTGATTATGGCGCAGCGTCTTTTACGCAGGCTGTGTTCGAGCTGCAAACAGG
AAGTGGAACGCCCGTCTGCCTCTGCTTTGAAGGAAGTCGGCTTCACCGATGAGGACCTTGCAAAAGATTGGAAACTTTAC
CGCGCCGTCGGTTGCGACCGTTGCCGGGGGCAGGGTTATAAGGGGCGTGCGGGCGTGTATGAGGTTATGCCCATCAGCGA
AGAAATGCAGCGTGTGATTATGAACAACGGTACGGAAGTGGATATTTTGGACGTTGCCTATAAGGAGGGTATGGTGGATT
TGCGCCGGGCCGGTATTTTGAAAGTTATGCAGGGCATTACTTCATTGGAAGAGGTAACGGCAAATACCAACGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(178-446)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

99.283

100

0.993
  pilB Acinetobacter baumannii D1279779

50.534

100

0.509
  pilB Acinetobacter baylyi ADP1

50.356

100

0.507
  pilB Legionella pneumophila strain ERS1305867

47.527

100

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

45.567

100

0.461
  pilB Vibrio cholerae strain A1552

45.374

100

0.457
  pilB Vibrio campbellii strain DS40M4

44.346

100

0.45
  pilB/pilB1 Synechocystis sp. PCC 6803

37.889

100

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.962

93.728

0.375
  pilF Thermus thermophilus HB27

36.949

97.491

0.36

Multiple sequence alignment