Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EL252_RS05810 Genome accession   NZ_LR134514
Coordinates   1262076..1263464 (-) Length   462 a.a.
NCBI ID   WP_115097850.1    Uniprot ID   -
Organism   Pasteurella multocida subsp. septica strain NCTC11619     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1257076..1268464
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL252_RS05780 (NCTC11619_01160) - 1257203..1258576 (-) 1374 WP_096743226.1 alanine/glycine:cation symporter family protein -
  EL252_RS05785 (NCTC11619_01162) - 1259025..1259294 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  EL252_RS05790 (NCTC11619_01163) yacG 1259294..1259497 (-) 204 WP_083004817.1 DNA gyrase inhibitor YacG -
  EL252_RS05795 (NCTC11619_01164) coaE 1259508..1260128 (-) 621 WP_096743227.1 dephospho-CoA kinase -
  EL252_RS05800 (NCTC11619_01165) - 1260140..1260865 (-) 726 WP_096743228.1 prepilin peptidase -
  EL252_RS05805 (NCTC11619_01166) pilC 1260865..1262076 (-) 1212 WP_126417627.1 type II secretion system F family protein Machinery gene
  EL252_RS05810 (NCTC11619_01167) pilB 1262076..1263464 (-) 1389 WP_115097850.1 GspE/PulE family protein Machinery gene
  EL252_RS05815 (NCTC11619_01168) pilA 1263464..1263898 (-) 435 WP_126417628.1 prepilin peptidase-dependent pilin Machinery gene
  EL252_RS05820 (NCTC11619_01169) ampD 1264058..1264624 (+) 567 WP_104227618.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EL252_RS05825 (NCTC11619_01170) rppH 1265210..1265806 (+) 597 WP_083004799.1 RNA pyrophosphohydrolase -
  EL252_RS05830 (NCTC11619_01171) - 1265809..1266603 (+) 795 WP_083004796.1 sulfite exporter TauE/SafE family protein -
  EL252_RS05835 (NCTC11619_01172) lgt 1266613..1267416 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  EL252_RS05840 (NCTC11619_01173) - 1267422..1268273 (+) 852 WP_126417629.1 thymidylate synthase -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 52091.68 Da        Isoelectric Point: 6.6308

>NTDB_id=1123664 EL252_RS05810 WP_115097850.1 1262076..1263464(-) (pilB) [Pasteurella multocida subsp. septica strain NCTC11619]
MFHKNPLYTVSATTGEVFEIPQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPSKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRLQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSVEKHILTAEDPIEIALSGIIQTQVNTQIGLDFKRLLRT
FLRQDPDVIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCCDCS
VETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=1123664 EL252_RS05810 WP_115097850.1 1262076..1263464(-) (pilB) [Pasteurella multocida subsp. septica strain NCTC11619]
ATGTTTCATAAGAATCCCTTATATACTGTTAGCGCGACGACAGGAGAGGTATTTGAAATCCCGCAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGCTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCTGCATGTGATGCCTTTTCTTTTTTAACCAATAAACACATTGAACCTGTTTTGTTATCA
ACAAATGAACTTAAACGGGCATTGCAGCAACTTTCCCCATCAAAATTACAAGTGGAAGAAAACATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGATATTCATTTTGAACCGCAACAGCATAGTTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTTCCTTTTCAGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACGTTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTACAGCGAAATAAGCCTATTCATCTCAGTTTTGCCGAGTTGGGGATG
ACCGCAGACCAACAACACAGGCTCCAACACGCGTTAAGTCAGCCTCAAGGACTGATTTTAGTCACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTACAGTGCGTTAGCTTGGCTCAATAGCGTAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGAGCGGGATAATCCAAACGCAAGTCAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACGTTATTATGTTAGGGGAAATTCGTGATGAGGAAAGTGCGTTAATCGCATTACGTGCAGC
TCAAACTGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTATTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTTGTGATTGCAGT
GTAGAGACACAATCGGCGTGTCGTTGCTATCAAGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAA
AACAGGGTGTTTTGTCACGGATTTTAGTAGTTTACGTGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACTGATGACA
ATGAAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(16-95)

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

67.77

98.052

0.664
  pilB Haemophilus influenzae Rd KW20

67.108

98.052

0.658
  pilB Glaesserella parasuis strain SC1401

58.134

99.784

0.58
  pilB Vibrio parahaemolyticus RIMD 2210633

40.125

100

0.418
  pilB Acinetobacter baumannii D1279779

39.148

100

0.418
  pilB Vibrio campbellii strain DS40M4

39.419

100

0.411
  pilB Vibrio cholerae strain A1552

38.683

100

0.407
  pilB Acinetobacter baylyi ADP1

37.475

100

0.405
  pilB Legionella pneumophila strain ERS1305867

36.605

100

0.387

Multiple sequence alignment