Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EL252_RS05815 Genome accession   NZ_LR134514
Coordinates   1263464..1263898 (-) Length   144 a.a.
NCBI ID   WP_126417628.1    Uniprot ID   -
Organism   Pasteurella multocida subsp. septica strain NCTC11619     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1258464..1268898
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL252_RS05785 (NCTC11619_01162) - 1259025..1259294 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  EL252_RS05790 (NCTC11619_01163) yacG 1259294..1259497 (-) 204 WP_083004817.1 DNA gyrase inhibitor YacG -
  EL252_RS05795 (NCTC11619_01164) coaE 1259508..1260128 (-) 621 WP_096743227.1 dephospho-CoA kinase -
  EL252_RS05800 (NCTC11619_01165) - 1260140..1260865 (-) 726 WP_096743228.1 prepilin peptidase -
  EL252_RS05805 (NCTC11619_01166) pilC 1260865..1262076 (-) 1212 WP_126417627.1 type II secretion system F family protein Machinery gene
  EL252_RS05810 (NCTC11619_01167) pilB 1262076..1263464 (-) 1389 WP_115097850.1 GspE/PulE family protein Machinery gene
  EL252_RS05815 (NCTC11619_01168) pilA 1263464..1263898 (-) 435 WP_126417628.1 prepilin peptidase-dependent pilin Machinery gene
  EL252_RS05820 (NCTC11619_01169) ampD 1264058..1264624 (+) 567 WP_104227618.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EL252_RS05825 (NCTC11619_01170) rppH 1265210..1265806 (+) 597 WP_083004799.1 RNA pyrophosphohydrolase -
  EL252_RS05830 (NCTC11619_01171) - 1265809..1266603 (+) 795 WP_083004796.1 sulfite exporter TauE/SafE family protein -
  EL252_RS05835 (NCTC11619_01172) lgt 1266613..1267416 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  EL252_RS05840 (NCTC11619_01173) - 1267422..1268273 (+) 852 WP_126417629.1 thymidylate synthase -
  EL252_RS05845 (NCTC11619_01174) tadA 1268300..1268788 (+) 489 WP_108511809.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15100.52 Da        Isoelectric Point: 8.8040

>NTDB_id=1123665 EL252_RS05815 WP_126417628.1 1263464..1263898(-) (pilA) [Pasteurella multocida subsp. septica strain NCTC11619]
MKKAIFFSIQKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGVSYTLTASGNAVQGVSWTVNCGAQSDLFPAGFCV

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1123665 EL252_RS05815 WP_126417628.1 1263464..1263898(-) (pilA) [Pasteurella multocida subsp. septica strain NCTC11619]
ATGAAAAAAGCCATTTTCTTTTCTATTCAAAAAGGGTTTACCCTCATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCGGCAGCAAGTAAAAAGTATTTAAAAGCCATCACGGTTAAAGCAGGGGTAATTACTGTTACTGGACAAGGGAG
TTTGGAAGGAGTTAGCTATACGCTGACAGCGAGTGGTAACGCAGTTCAAGGTGTTTCTTGGACCGTTAATTGTGGTGCTC
AATCTGACCTTTTCCCTGCAGGATTTTGTGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Pilin (bacterial filament) (Pilin)

(42-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

62.5

100

0.625
  pilA Haemophilus influenzae Rd KW20

60.417

100

0.604
  pilA Glaesserella parasuis strain SC1401

53.02

100

0.549
  pilA Vibrio campbellii strain DS40M4

36

100

0.375

Multiple sequence alignment