Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EL242_RS08480 Genome accession   NZ_LR134495
Coordinates   1719592..1720995 (-) Length   467 a.a.
NCBI ID   WP_126302231.1    Uniprot ID   A0A3S5BCH6
Organism   Mannheimia haemolytica strain NCTC10643     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1714592..1725995
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL242_RS08450 (NCTC10643_01678) lptE 1715354..1715842 (+) 489 WP_126302225.1 LPS assembly lipoprotein LptE -
  EL242_RS08455 (NCTC10643_01679) holA 1715885..1716910 (+) 1026 WP_126302226.1 DNA polymerase III subunit delta -
  EL242_RS08460 (NCTC10643_01680) yacG 1716912..1717097 (-) 186 WP_126302227.1 DNA gyrase inhibitor YacG -
  EL242_RS08465 (NCTC10643_01681) coaE 1717079..1717717 (-) 639 WP_126302228.1 dephospho-CoA kinase -
  EL242_RS08470 (NCTC10643_01682) - 1717753..1718409 (-) 657 WP_126302229.1 prepilin peptidase -
  EL242_RS08475 (NCTC10643_01683) pilC 1718412..1719608 (-) 1197 WP_126302230.1 type II secretion system F family protein Machinery gene
  EL242_RS08480 (NCTC10643_01684) pilB 1719592..1720995 (-) 1404 WP_126302231.1 GspE/PulE family protein Machinery gene
  EL242_RS08485 (NCTC10643_01685) pilA 1721016..1721495 (-) 480 WP_126302232.1 prepilin peptidase-dependent pilin Machinery gene
  EL242_RS08495 (NCTC10643_01686) radA 1721676..1723055 (+) 1380 WP_126302233.1 DNA repair protein RadA -
  EL242_RS08500 (NCTC10643_01687) - 1723110..1723340 (-) 231 WP_126302234.1 YgjV family protein -
  EL242_RS08505 (NCTC10643_01688) - 1723460..1723954 (-) 495 WP_126302235.1 dihydrofolate reductase -
  EL242_RS08510 (NCTC10643_01689) proB 1724066..1725157 (+) 1092 WP_126302236.1 glutamate 5-kinase -
  EL242_RS08515 (NCTC10643_01690) - 1725157..1725921 (+) 765 WP_126302237.1 TSUP family transporter -

Sequence

Protein


Download         Length: 467 a.a.        Molecular weight: 52924.47 Da        Isoelectric Point: 5.1521

>NTDB_id=1123268 EL242_RS08480 WP_126302231.1 1719592..1720995(-) (pilB) [Mannheimia haemolytica strain NCTC10643]
MEYSVTDLNSQRFIEISAEQWQKNCNEKQILLRYLAVPLQETEEQLWLGIDDMQNLNACEIFAFIYHKQIEPVLISSEEL
KFLLNALSPEQSSQQSEIYEENPLYPFSENNEQPDRNDPIIQILENLFKFCLQQNASDIHLEPQKETLLIRLRIDGVLHI
YKSLPSSLSSRLISRVKLLAKLDISETRLPQDGQFHFKTLLAETLDFRVSTLPTHFGEKVVLRLQKNKPTHFDFLDLGFN
PAQKANLLNALSQPQGLILVTGPTGSGKSITLYSALSHLNSSEKHILTAEDPIEIEIEGIIQTQVNRGINLDFSQLLRTF
LRQDPDIIMLGEIRDEESAEMALRAAQTGHLVLSTLHTNDAPSAVERLLQLGIKEYELKNSLLLVIAQRLLRRVCVKCGG
AGCEHCYQGYKGQIGVYQLLNRSAKNFEKNTACLDFKTLADSAKEKLAEGKTNQAEILRVLGNDENL

Nucleotide


Download         Length: 1404 bp        

>NTDB_id=1123268 EL242_RS08480 WP_126302231.1 1719592..1720995(-) (pilB) [Mannheimia haemolytica strain NCTC10643]
ATGGAGTATTCGGTAACCGACTTAAACAGCCAAAGGTTCATTGAGATTTCAGCAGAGCAGTGGCAGAAAAATTGCAATGA
AAAACAGATTTTGTTGCGTTATTTAGCTGTTCCACTGCAAGAAACCGAAGAACAGCTTTGGCTAGGGATTGATGATATGC
AAAACCTCAATGCCTGCGAGATTTTTGCGTTTATCTACCACAAACAGATTGAACCTGTTTTGATTTCCTCCGAAGAATTA
AAATTTTTGCTCAATGCCCTTTCGCCTGAGCAGAGTAGCCAGCAGAGTGAAATTTATGAAGAAAATCCGCTTTATCCTTT
TTCCGAGAATAACGAGCAGCCGGATAGAAATGATCCGATTATCCAAATTTTAGAGAATTTGTTTAAGTTCTGTTTGCAAC
AGAACGCTTCCGATATCCATCTTGAACCACAAAAAGAGACATTATTAATTCGATTAAGAATTGATGGCGTGCTACATATT
TATAAAAGCTTGCCGAGTAGTTTATCCAGCCGTTTGATTTCACGGGTTAAATTATTGGCGAAGCTCGATATCAGCGAGAC
TCGCTTGCCACAAGACGGGCAATTTCATTTCAAAACACTGTTAGCTGAAACACTGGATTTCCGTGTTTCTACCTTGCCAA
CGCACTTCGGCGAAAAAGTGGTGCTACGGCTACAAAAAAATAAACCGACCCATTTTGATTTTTTAGATTTAGGCTTTAAC
CCGGCTCAGAAAGCCAATTTGCTGAATGCCCTCAGTCAGCCTCAAGGGTTGATTTTAGTAACTGGCCCGACTGGGAGCGG
TAAAAGTATCACGCTTTACAGTGCATTAAGCCATTTAAACAGCAGTGAAAAGCATATTTTGACTGCCGAAGACCCGATTG
AAATTGAGATTGAGGGAATTATCCAAACCCAAGTGAATCGAGGGATTAACTTAGATTTCAGCCAACTGTTAAGAACCTTT
CTACGCCAAGATCCGGACATTATTATGCTCGGCGAAATTCGTGATGAAGAGAGTGCAGAAATGGCACTAAGGGCAGCACA
AACCGGGCATTTGGTGCTTTCGACTTTGCACACCAATGATGCCCCTTCAGCGGTGGAACGGTTGCTACAGCTCGGCATAA
AAGAGTATGAACTGAAAAATTCATTGCTACTCGTGATTGCTCAACGCCTGTTACGCCGAGTGTGTGTGAAATGCGGTGGG
GCAGGCTGTGAGCATTGTTATCAAGGGTATAAAGGGCAAATTGGGGTATATCAACTCTTAAACCGATCTGCAAAAAATTT
TGAAAAAAACACCGCTTGTTTGGATTTTAAAACCCTTGCCGACAGTGCAAAAGAAAAATTGGCGGAAGGAAAAACCAATC
AGGCAGAAATTTTAAGGGTGCTAGGCAATGACGAAAATCTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-93)

Type II/IV secretion system protein (T2SSE)

(132-393)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S5BCH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

72.451

98.715

0.715
  pilB Haemophilus influenzae Rd KW20

56.494

98.929

0.559
  pilB Haemophilus influenzae 86-028NP

55.724

99.143

0.552
  pilB Vibrio cholerae strain A1552

40.249

100

0.415
  pilB Vibrio campbellii strain DS40M4

40.125

100

0.413
  pilB Legionella pneumophila strain ERS1305867

39.549

100

0.413
  pilB Vibrio parahaemolyticus RIMD 2210633

38.554

100

0.411
  pilB Acinetobacter baumannii D1279779

36.049

100

0.379
  pilB Acinetobacter baylyi ADP1

36.066

100

0.377

Multiple sequence alignment