Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EL242_RS08475 Genome accession   NZ_LR134495
Coordinates   1718412..1719608 (-) Length   398 a.a.
NCBI ID   WP_126302230.1    Uniprot ID   A0A448TDE1
Organism   Mannheimia haemolytica strain NCTC10643     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1713412..1724608
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL242_RS08450 (NCTC10643_01678) lptE 1715354..1715842 (+) 489 WP_126302225.1 LPS assembly lipoprotein LptE -
  EL242_RS08455 (NCTC10643_01679) holA 1715885..1716910 (+) 1026 WP_126302226.1 DNA polymerase III subunit delta -
  EL242_RS08460 (NCTC10643_01680) yacG 1716912..1717097 (-) 186 WP_126302227.1 DNA gyrase inhibitor YacG -
  EL242_RS08465 (NCTC10643_01681) coaE 1717079..1717717 (-) 639 WP_126302228.1 dephospho-CoA kinase -
  EL242_RS08470 (NCTC10643_01682) - 1717753..1718409 (-) 657 WP_126302229.1 prepilin peptidase -
  EL242_RS08475 (NCTC10643_01683) pilC 1718412..1719608 (-) 1197 WP_126302230.1 type II secretion system F family protein Machinery gene
  EL242_RS08480 (NCTC10643_01684) pilB 1719592..1720995 (-) 1404 WP_126302231.1 GspE/PulE family protein Machinery gene
  EL242_RS08485 (NCTC10643_01685) pilA 1721016..1721495 (-) 480 WP_126302232.1 prepilin peptidase-dependent pilin Machinery gene
  EL242_RS08495 (NCTC10643_01686) radA 1721676..1723055 (+) 1380 WP_126302233.1 DNA repair protein RadA -
  EL242_RS08500 (NCTC10643_01687) - 1723110..1723340 (-) 231 WP_126302234.1 YgjV family protein -
  EL242_RS08505 (NCTC10643_01688) - 1723460..1723954 (-) 495 WP_126302235.1 dihydrofolate reductase -

Sequence

Protein


Download         Length: 398 a.a.        Molecular weight: 45287.84 Da        Isoelectric Point: 9.7839

>NTDB_id=1123267 EL242_RS08475 WP_126302230.1 1718412..1719608(-) (pilC) [Mannheimia haemolytica strain NCTC10643]
MTKIYEFHWKAQSRFGQKQRGKRLATNREMLENLLLAQGYQHIRISRNFVLSTNPKKEEVNSLLSQLALLVNSAIPLKQA
LSMLLQNCRNIQLYQWLNELIQHIESGYAFSQSLEKLGKFVTNQEIQLIKMGEQSGKLGIMLTNLAESRAKSEKLAKKVK
KILFYPVIVLLISLTLSIGLLVLIVPQFAQLYGSKDKTLPLITDILFTLSECLIEQGSVLLGTLLLAVIFYLFFAKKSRL
VAKWKMALWAKMPLFRRIIEQSRIIFFSQNIALMLNAHIPLDVTLRSFLSEKNDDPILQQEIGTMLNLLQQGYKFSQGIN
PNVFGAEMAQMLEVGEKSGNVAKMCEHISEMYQQKLDYQIDILSQLLEPMLMLIMGVIVGTIIVGLYLPIFDMGALVE

Nucleotide


Download         Length: 1197 bp        

>NTDB_id=1123267 EL242_RS08475 WP_126302230.1 1718412..1719608(-) (pilC) [Mannheimia haemolytica strain NCTC10643]
ATGACGAAAATCTATGAATTTCATTGGAAAGCCCAGAGCCGATTCGGGCAGAAACAGCGTGGAAAGCGGTTGGCAACGAA
TCGAGAAATGTTGGAAAACCTTTTGCTGGCACAAGGTTATCAGCACATTCGGATTAGCCGAAATTTTGTGCTTTCAACGA
ACCCGAAAAAAGAAGAGGTGAACAGCCTGTTATCGCAACTGGCGTTGTTGGTTAATTCTGCCATTCCGTTAAAACAGGCA
TTGTCTATGTTGTTGCAAAACTGCCGAAATATCCAGCTTTATCAATGGCTTAATGAGCTGATTCAGCATATTGAAAGTGG
CTATGCCTTTTCACAATCGTTGGAGAAATTGGGTAAATTTGTGACCAATCAAGAAATTCAACTAATCAAAATGGGCGAGC
AAAGCGGAAAACTGGGCATTATGCTGACTAATTTAGCTGAAAGCCGAGCAAAATCGGAAAAATTGGCGAAAAAAGTGAAG
AAAATCCTGTTCTACCCGGTGATAGTGTTGTTGATTTCGCTCACTTTATCTATCGGGCTATTGGTCTTGATTGTACCGCA
GTTTGCCCAACTTTATGGCTCAAAAGACAAAACTTTACCCCTCATTACCGATATTTTATTTACCCTTTCCGAATGCTTGA
TTGAGCAAGGTTCAGTGTTGCTTGGCACGCTACTACTAGCGGTCATTTTTTACCTGTTTTTTGCAAAAAAGAGCAGGTTA
GTCGCAAAGTGGAAAATGGCTCTATGGGCTAAAATGCCACTGTTTAGACGAATTATTGAGCAATCTCGCATTATCTTTTT
CAGCCAAAACATTGCCTTAATGCTGAATGCCCATATTCCGCTTGATGTTACCTTGAGGTCGTTTTTATCCGAGAAAAATG
ATGACCCGATTTTACAGCAAGAAATTGGCACTATGCTTAATTTGTTACAACAAGGCTACAAATTTTCACAAGGGATCAAC
CCGAATGTGTTTGGGGCTGAGATGGCACAAATGCTTGAGGTTGGGGAGAAAAGTGGTAATGTGGCGAAAATGTGTGAGCA
TATTAGTGAGATGTACCAACAAAAATTAGATTACCAAATTGATATTTTGTCTCAGCTATTAGAACCGATGCTAATGTTGA
TAATGGGCGTGATTGTCGGCACTATTATTGTCGGGCTGTATTTACCGATTTTTGATATGGGTGCTTTGGTGGAGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(267-389)

Type II secretion system (T2SS), protein F (T2SSF)

(64-186)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A448TDE1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Glaesserella parasuis strain SC1401

56

100

0.563
  pilC Haemophilus influenzae Rd KW20

42.145

100

0.425
  pilC Haemophilus influenzae 86-028NP

41.147

100

0.415

Multiple sequence alignment