Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   S7S_RS04910 Genome accession   NZ_CP004387
Coordinates   1041900..1043606 (-) Length   568 a.a.
NCBI ID   WP_035205748.1    Uniprot ID   A0A0B4XJZ1
Organism   Isoalcanivorax pacificus W11-5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1036900..1048606
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  S7S_RS04885 (S7S_04870) - 1037923..1038834 (+) 912 WP_008739560.1 Nudix family hydrolase -
  S7S_RS04890 (S7S_04875) yacG 1038883..1039065 (-) 183 WP_008739561.1 DNA gyrase inhibitor YacG -
  S7S_RS04895 (S7S_04880) coaE 1039070..1039684 (-) 615 WP_008739562.1 dephospho-CoA kinase -
  S7S_RS04900 (S7S_04885) pilD 1039783..1040655 (-) 873 WP_008739563.1 prepilin peptidase Machinery gene
  S7S_RS04905 (S7S_04890) pilC 1040668..1041897 (-) 1230 WP_008739564.1 type II secretion system F family protein Machinery gene
  S7S_RS04910 (S7S_04895) pilB 1041900..1043606 (-) 1707 WP_035205748.1 type IV-A pilus assembly ATPase PilB Machinery gene
  S7S_RS20170 - 1044093..1044210 (+) 118 Protein_985 pilin -
  S7S_RS04915 (S7S_04900) - 1044222..1044560 (+) 339 WP_420795643.1 pilin -
  S7S_RS04920 (S7S_04905) - 1044786..1045946 (+) 1161 WP_169745548.1 serine hydrolase domain-containing protein -
  S7S_RS04925 (S7S_04910) - 1046193..1047287 (+) 1095 WP_202966526.1 acyltransferase family protein -
  S7S_RS04930 (S7S_04915) - 1047435..1048427 (+) 993 WP_008739573.1 transposase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62143.20 Da        Isoelectric Point: 5.4917

>NTDB_id=112323 S7S_RS04910 WP_035205748.1 1041900..1043606(-) (pilB) [Isoalcanivorax pacificus W11-5]
MSTQLSGLARRLVSESVLAEDRAREALAGARQEKLSLVTYIVRKGLARAADVSRIVAEEFGDPVIDLAAFNPEMLIKGLV
DHKLVEKHRALPLFRRGSRLFVAVSDPTNLQALEDLRFNTGLNIETIIAEEDKLGQLIDAVVNEAEGGAFKDLDEGLDDI
DISGGEAAQEGAEDVASASDDAPIVRFVNKLLLDAIKIGASDLHFEPYEKTYRVRFRQDGILREIAKPPANSAGKIAARL
KVMSQLDISERRVPQDGRIKLKVSKTRAIDFRVNTCPTLFGEKIVLRILDPESAKMGIDALGYEEGQKKLYMDALSKPQG
MILVTGPTGSGKTVSLYTGVNILNTPERNISTAEDPVEINLEGINQVNVNTKTGMDFSAALKAFLRQDPDVILVGEIRDL
ETASIAVKAAQTGHLVLSTLHTNSAPETLTRLRNMGVPSFNIATSVILIIAQRLARRLCEHCKQPVEVPRQSLLEMGFTE
EDLAQPFTVYGPKGCEKCNNGYKGRVGIYEVVRITDGIARIIMEEGNSIQIADQCRKEGFNDLSRSGLVKVMQGMTSLEE
VNRVTSGH

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=112323 S7S_RS04910 WP_035205748.1 1041900..1043606(-) (pilB) [Isoalcanivorax pacificus W11-5]
ATGTCGACGCAATTGAGCGGTCTTGCCCGCAGGCTTGTCAGTGAATCCGTGCTCGCAGAAGACCGAGCGCGCGAGGCGCT
GGCTGGCGCCCGTCAGGAAAAACTCAGCCTCGTCACCTATATTGTCCGCAAGGGCCTGGCGCGGGCCGCCGATGTCTCCC
GCATTGTGGCGGAAGAGTTTGGTGATCCGGTCATCGATCTGGCTGCCTTCAACCCCGAAATGCTGATCAAGGGGCTGGTG
GATCACAAGCTGGTCGAGAAGCACCGGGCGCTGCCGCTGTTCCGTCGTGGCAGCCGGCTGTTTGTCGCCGTCTCTGATCC
CACCAACCTGCAGGCCCTGGAAGACCTGCGTTTCAACACCGGCCTCAACATTGAGACCATCATCGCCGAGGAAGACAAAC
TCGGGCAGTTGATCGACGCTGTCGTCAATGAGGCCGAGGGTGGCGCCTTCAAGGATCTTGATGAGGGCCTGGACGACATT
GACATCAGCGGGGGGGAAGCCGCCCAGGAAGGGGCGGAGGATGTCGCCTCGGCGTCTGACGATGCCCCCATCGTCCGGTT
CGTCAACAAGCTGCTGCTGGACGCCATCAAGATCGGCGCGTCCGATCTGCACTTCGAGCCCTACGAAAAGACCTATCGCG
TGCGCTTTCGCCAGGACGGCATCCTGCGCGAGATCGCCAAGCCGCCCGCCAATAGTGCCGGCAAGATTGCCGCCCGTCTG
AAGGTCATGTCGCAGCTCGATATCTCCGAGCGCCGTGTCCCGCAGGACGGCCGGATCAAGCTGAAAGTATCCAAGACCCG
CGCCATCGACTTCCGCGTCAACACCTGCCCCACCCTGTTCGGGGAAAAGATCGTGCTGCGTATCCTCGACCCGGAAAGCG
CCAAGATGGGTATTGATGCGCTGGGCTATGAGGAAGGGCAGAAGAAGCTGTACATGGATGCCCTGAGCAAGCCGCAGGGC
ATGATCCTGGTGACCGGACCGACCGGCTCCGGCAAGACCGTTTCCCTGTACACCGGGGTCAATATCCTCAACACGCCGGA
GCGTAATATCTCCACGGCGGAAGACCCGGTGGAGATCAACCTGGAAGGTATCAACCAGGTCAACGTGAACACGAAGACCG
GCATGGACTTCTCCGCTGCCCTGAAAGCCTTCCTGCGGCAGGACCCGGACGTGATCCTGGTGGGCGAGATCCGTGACCTG
GAAACCGCCAGCATCGCGGTGAAAGCAGCCCAGACCGGTCACCTGGTGCTCTCTACCCTGCACACAAACAGTGCGCCGGA
GACCCTGACCCGCCTGCGCAATATGGGGGTGCCGTCGTTCAATATCGCCACGTCGGTCATCCTGATCATCGCCCAGCGTC
TGGCCCGGCGGCTGTGCGAACATTGCAAGCAGCCGGTGGAGGTGCCCCGCCAGTCGCTGCTGGAGATGGGCTTTACCGAG
GAAGATCTCGCGCAGCCTTTCACCGTGTACGGCCCCAAAGGGTGCGAGAAGTGCAATAACGGCTATAAAGGACGCGTGGG
CATCTATGAGGTGGTCAGAATCACCGATGGTATCGCCCGCATCATCATGGAAGAAGGCAACTCCATCCAGATCGCGGACC
AATGCCGCAAGGAAGGCTTCAACGACCTGTCCCGTTCCGGGCTGGTCAAGGTCATGCAGGGCATGACCAGCCTTGAAGAA
GTCAACCGTGTAACCTCCGGACACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B4XJZ1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

62.081

99.824

0.62
  pilB Acinetobacter baylyi ADP1

60.993

99.296

0.606
  pilB Legionella pneumophila strain ERS1305867

53.887

99.648

0.537
  pilF Neisseria gonorrhoeae MS11

49.472

100

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

47.751

100

0.486
  pilB Vibrio campbellii strain DS40M4

48.677

99.824

0.486
  pilB Vibrio cholerae strain A1552

51.81

92.43

0.479
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.176

100

0.398
  pilB/pilB1 Synechocystis sp. PCC 6803

41.6

88.028

0.366
  pilF Thermus thermophilus HB27

37.798

95.951

0.363

Multiple sequence alignment