Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EL330_RS17695 Genome accession   NZ_LR134482
Coordinates   3742276..3744363 (-) Length   695 a.a.
NCBI ID   WP_101276060.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain NCTC10475     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3737276..3749363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL330_RS17680 (NCTC10475_03553) - 3739001..3740587 (-) 1587 WP_101276058.1 AAA family ATPase -
  EL330_RS17685 (NCTC10475_03554) aroB 3740598..3741701 (-) 1104 WP_042926348.1 3-dehydroquinate synthase -
  EL330_RS17690 (NCTC10475_03555) aroK 3741725..3742270 (-) 546 WP_014821719.1 shikimate kinase AroK -
  EL330_RS17695 (NCTC10475_03556) pilQ 3742276..3744363 (-) 2088 WP_101276060.1 type IV pilus secretin PilQ Machinery gene
  EL330_RS17700 (NCTC10475_03557) pilP 3744417..3744944 (-) 528 WP_101276062.1 type 4a pilus biogenesis lipoprotein PilP -
  EL330_RS17705 (NCTC10475_03558) pilO 3744941..3745564 (-) 624 WP_101276064.1 type 4a pilus biogenesis protein PilO -
  EL330_RS17710 (NCTC10475_03559) pilN 3745561..3746133 (-) 573 WP_014821721.1 type 4a pilus biogenesis protein PilN -
  EL330_RS17715 (NCTC10475_03560) pilM 3746133..3747197 (-) 1065 WP_023446117.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 74683.76 Da        Isoelectric Point: 4.7742

>NTDB_id=1123074 EL330_RS17695 WP_101276060.1 3742276..3744363(-) (pilQ) [Stutzerimonas stutzeri strain NCTC10475]
MNTTLSRLGISLLAAFISPALLAANLNALDVAALPGDRVELKLAFDEPVATPRGYTLDQPARIALDLPGVSNKLGAKNRE
LGVGNARSVTVVEAQGRTRLIVNLNTLVPYSTRVDGNNLFVVLGESSGAVRAPAVSAAPVAPVAAPAKAMAVGKAIENID
FRRGEDGAGNVVITLSDPSVGSTIEEQGGKIRVLFDKAQLPEALRVRLDVQDFATPVKFVDSSAQAGGASVAIEPTGRYD
YLAYQTDNKLTISIKPLTEDEAEKRKAERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVAGNITLRLQNVPW
DQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQRQIAELAPLRREVVQVNYAKAADIARLFQSVTDNGGVAE
ERGSIAVDDRTNNIIAYQTQDRLDELRRIVTQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGNLGAGDKWSAWGKNGNL
DVEDAEGGGQRITWPMNTPFVDMGAVGATSGLGLGFVTDNAILDLQLSAMEKTGNGEVVSQPKVVTADKETAKILKGSEV
PYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDEPDFSQAATTGVPAIRKNEVNAKVLVTDGETIVIGGV
FSNTQSKSVDKVPLLGDLPFVGRLFRRDLVQDRKSELLVFITPRIMNNQAIQASR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=1123074 EL330_RS17695 WP_101276060.1 3742276..3744363(-) (pilQ) [Stutzerimonas stutzeri strain NCTC10475]
ATGAACACTACCCTTTCACGCCTAGGTATCTCTTTGCTGGCGGCGTTTATTTCTCCAGCCCTGCTGGCGGCCAACCTGAA
CGCCCTCGATGTCGCTGCGCTGCCTGGCGACCGTGTTGAATTGAAGCTTGCGTTCGACGAACCGGTGGCTACACCGCGTG
GTTATACCCTCGATCAGCCTGCGCGGATCGCGCTCGACCTACCCGGCGTATCCAATAAGCTCGGAGCGAAGAATCGCGAG
CTAGGAGTTGGCAATGCGCGGAGCGTGACCGTCGTTGAAGCCCAAGGCCGTACCCGACTGATCGTTAATCTCAATACTCT
GGTGCCCTACTCCACTCGCGTAGATGGTAACAATCTCTTTGTGGTATTGGGCGAAAGTTCGGGTGCTGTACGTGCCCCAG
CTGTTTCAGCTGCGCCCGTTGCTCCAGTGGCTGCGCCTGCCAAGGCGATGGCTGTAGGTAAGGCGATCGAGAATATCGAT
TTCCGTCGGGGTGAAGACGGTGCCGGAAACGTCGTTATTACGCTCTCTGACCCATCGGTAGGCTCGACCATTGAGGAGCA
GGGCGGAAAGATTCGTGTCCTCTTCGACAAGGCTCAACTGCCAGAGGCGCTGCGTGTTCGTCTTGATGTTCAGGATTTCG
CTACGCCCGTGAAATTTGTCGATTCGTCCGCTCAGGCCGGTGGAGCTAGTGTCGCCATCGAGCCGACCGGTCGATACGAT
TATCTGGCGTACCAGACAGACAACAAACTGACCATCAGCATCAAGCCTCTGACCGAGGATGAAGCCGAGAAGCGCAAGGC
TGAGCGTTTTGCTTATTCGGGCGAGAAACTCTCGCTCAACTTTCAGGATATCGACGTTCGTTCCGTACTGCAGCTGATCG
CTGACTTCACCGATCTGAACTTGGTGGCGAGCGATACCGTCGCTGGCAACATAACCCTGCGTCTGCAAAACGTGCCTTGG
GACCAGGCGCTGGATCTGGTGCTTAAAACCAAAGGTCTGGATAAGCGCCAGGTTGGTAACGTGCTGCTGGTCGCGCCTGC
CGACGAGATTGCTGCCCGTGAGCGTCAGGAGCTTGAGTCACAGCGGCAGATCGCCGAGCTTGCTCCACTTCGTCGCGAGG
TCGTTCAGGTCAATTACGCAAAGGCTGCCGATATTGCGCGGCTGTTTCAGTCGGTGACGGACAATGGTGGAGTGGCTGAA
GAGCGTGGTTCTATTGCAGTCGATGATCGTACCAACAATATCATCGCCTATCAGACCCAGGATCGGCTGGACGAGCTACG
CCGAATCGTTACGCAGTTGGACATTCCGGTCCGTCAGGTAATGATCGAAGCGCGAATAGTTGAAGCAAACGTTGACTACG
ACAAAAGCCTCGGCGTCCGTTGGGGCGGTAATCTTGGCGCTGGCGATAAGTGGAGTGCTTGGGGTAAGAACGGAAATCTA
GATGTCGAGGATGCTGAGGGTGGCGGCCAGCGGATTACCTGGCCAATGAATACTCCGTTTGTTGACATGGGGGCAGTTGG
AGCGACGTCTGGGTTAGGGTTGGGGTTCGTTACAGATAATGCAATTCTGGACTTGCAGCTTTCCGCAATGGAGAAAACTG
GTAATGGAGAGGTTGTATCGCAACCTAAAGTAGTTACCGCAGACAAAGAAACAGCAAAAATTTTGAAAGGCTCTGAGGTT
CCTTATCAGGAAGCCAGTTCGAGCGGTGCCACCAGCACATCGTTCAAGGAGGCTGCACTTTCGTTGGAGGTCACGCCGCA
GATTACCCCGGATAATCGAATTATCATGGAAGTCAAGGTTACTAAAGACGAGCCCGACTTTTCACAGGCGGCTACGACTG
GTGTTCCAGCAATCCGTAAGAACGAAGTTAATGCGAAGGTCCTAGTGACTGATGGCGAAACGATTGTCATCGGCGGTGTC
TTTTCCAATACGCAGAGCAAGTCCGTGGATAAGGTTCCTTTGCTAGGTGATCTTCCCTTCGTAGGGCGTCTATTCCGTCG
TGATCTGGTGCAGGATCGAAAATCGGAGCTGCTTGTGTTCATCACGCCTCGGATCATGAATAATCAGGCGATTCAAGCCA
GTCGCTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(373-439)

Bacterial type II and III secretion system protein (Secretin)

(529-686)

AMIN domain (AMIN)

(38-123)

Secretin and TonB N terminus short domain (STN)

(300-346)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.955

100

0.764
  comQ Acinetobacter baylyi ADP1

41.547

100

0.417
  pilQ Acinetobacter baumannii strain A118

39.886

100

0.403
  pilQ Acinetobacter baumannii D1279779

39.886

100

0.403
  pilQ Legionella pneumophila strain Lp02

37.389

96.978

0.363
  pilQ Legionella pneumophila strain ERS1305867

37.389

96.978

0.363

Multiple sequence alignment