Detailed information NTG1123075

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: EL330_RS17715
• Genome accession: NZ_LR134482
• Coordinates: 3746133..3747197 (-)
• Length: 354 a.a.
• NCBI ID: WP_023446117.1
• Uniprot ID: V4QEI0
• Organism: Stutzerimonas stutzeri strain NCTC10475
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3741133..3752197

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL330_RS17690 (NCTC10475_03555)	aroK	3741725..3742270 (-)	546	WP_014821719.1	shikimate kinase AroK	-
EL330_RS17695 (NCTC10475_03556)	pilQ	3742276..3744363 (-)	2088	WP_101276060.1	type IV pilus secretin PilQ	Machinery gene
EL330_RS17700 (NCTC10475_03557)	pilP	3744417..3744944 (-)	528	WP_101276062.1	type 4a pilus biogenesis lipoprotein PilP	-
EL330_RS17705 (NCTC10475_03558)	pilO	3744941..3745564 (-)	624	WP_101276064.1	type 4a pilus biogenesis protein PilO	-
EL330_RS17710 (NCTC10475_03559)	pilN	3745561..3746133 (-)	573	WP_014821721.1	type 4a pilus biogenesis protein PilN	-
EL330_RS17715 (NCTC10475_03560)	pilM	3746133..3747197 (-)	1065	WP_023446117.1	pilus assembly protein PilM	Machinery gene
EL330_RS17720 (NCTC10475_03561)	-	3747378..3749810 (+)	2433	WP_101276066.1	penicillin-binding protein 1A	-
EL330_RS17725 (NCTC10475_03562)	-	3749874..3751142 (-)	1269	WP_014821724.1	malic enzyme-like NAD(P)-binding protein	-
EL330_RS17730 (NCTC10475_03563)	-	3751292..3752086 (-)	795	WP_101276068.1	thermonuclease family protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37671.17 Da
• Isoelectric Point: 4.6880

>NTDB_id=1123075 EL330_RS17715 WP_023446117.1 3746133..3747197(-) (pilM) [Stutzerimonas stutzeri strain NCTC10475]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGSRYRVEAYAVEPLPANAVVEKNIAELEGVGQALQRVVAKAKTGCKSAVV
AVSGSAVITKIIEMDAGLSDEELENQLKIEADQYIPYPLEEVAIDFEVQGPAPRSPGRAEVLLAACRKENVEIREAALAL
AGLTAKVVDVEAYALERSYGLLAPQLGAAHGELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSQ
EEAGLAKKQGGLPDDYDSEVLLPFKEAVVQQVSRSLQFFFAAGQFHDVDCILLAGGTASIPGLDHMIQQKIGTQTLVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1123075 EL330_RS17715 WP_023446117.1 3746133..3747197(-) (pilM) [Stutzerimonas stutzeri strain NCTC10475]
GTGCTAGGGCTCTTCACTAAGAAAGCGAACACGCTGCTTGGGATCGATATCAGTTCGACCTCAGTCAAGCTCCTCGAATT
AAGTCGATCAGGTAGTCGCTACCGCGTCGAGGCGTATGCTGTCGAGCCGCTCCCGGCAAACGCCGTAGTTGAAAAGAACA
TCGCCGAGCTCGAGGGCGTCGGCCAGGCATTGCAGCGGGTGGTTGCCAAGGCCAAGACCGGATGCAAGTCGGCGGTAGTG
GCTGTTTCAGGTTCCGCGGTAATCACCAAGATCATCGAGATGGATGCTGGTTTGTCCGACGAAGAGCTCGAGAATCAGCT
GAAGATCGAGGCGGACCAGTACATTCCCTATCCGCTGGAAGAAGTGGCGATCGACTTCGAAGTCCAGGGGCCTGCTCCTC
GCTCTCCTGGCCGAGCAGAAGTCCTGCTCGCCGCTTGCCGCAAGGAAAACGTCGAAATTCGTGAGGCAGCGCTTGCGCTG
GCGGGGCTCACTGCCAAGGTGGTTGACGTCGAAGCCTATGCGCTCGAGCGGTCTTACGGGCTACTCGCTCCACAGTTGGG
TGCGGCGCATGGAGAGCTGACCGTTGCCGTGGTGGATATCGGTGCCACCATGACTACCCTCAGTGTTCTGCATAACGGTC
GTACTATTTATACCCGTGAACAGCTTTTCGGCGGGCGTCAGCTGACGGAGGAAATTCAGCGACGCTACGGCCTCTCACAA
GAAGAAGCTGGTCTAGCCAAAAAGCAAGGCGGCCTCCCAGACGACTACGACAGTGAGGTTTTGCTTCCGTTCAAAGAGGC
AGTTGTGCAGCAGGTTTCTCGCTCGTTGCAGTTCTTCTTTGCGGCAGGACAGTTCCATGATGTCGACTGCATCCTGTTAG
CGGGCGGGACGGCATCGATACCTGGGTTGGATCACATGATTCAGCAGAAGATCGGTACTCAGACTCTAGTTGCGAATCCA
TTCGCCGATATGGCTTTGAGCAGCAAAGTGAATGCTGGAGCGCTGGCTAGCGATGCGCCGGCCTTGATGATTGCTTGTGG
CCTGGCGCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: V4QEI0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.65	100	0.557
comM	Acinetobacter nosocomialis M2	55.65	100	0.557
comM	Acinetobacter baylyi ADP1	53.107	100	0.531
pilM	Legionella pneumophila strain ERS1305867	47.175	100	0.472