Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   EL216_RS08560 Genome accession   NZ_LR134440
Coordinates   1819985..1820548 (-) Length   187 a.a.
NCBI ID   WP_085390949.1    Uniprot ID   -
Organism   Neisseria animaloris strain NCTC12228     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1814985..1825548
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL216_RS08540 (NCTC12228_01718) - 1815670..1817193 (-) 1524 WP_085390945.1 fumarate hydratase -
  EL216_RS08545 (NCTC12228_01719) - 1818044..1818460 (+) 417 WP_085390946.1 hypothetical protein -
  EL216_RS08550 (NCTC12228_01720) - 1818566..1819342 (-) 777 WP_126300857.1 fimb protein -
  EL216_RS08555 (NCTC12228_01721) - 1819360..1819905 (-) 546 WP_085390948.1 pilin -
  EL216_RS08560 (NCTC12228_01722) pilE 1819985..1820548 (-) 564 WP_085390949.1 pilin Machinery gene
  EL216_RS08565 (NCTC12228_01723) - 1821387..1821875 (-) 489 WP_085390950.1 disulfide bond formation protein B -
  EL216_RS08570 (NCTC12228_01724) - 1821954..1822835 (-) 882 WP_085390951.1 prephenate dehydrogenase -
  EL216_RS08575 (NCTC12228_01725) - 1822979..1823755 (-) 777 WP_085390952.1 DsbC family protein -
  EL216_RS08580 (NCTC12228_01726) - 1823980..1824774 (+) 795 WP_085390953.1 3'-5' exonuclease -

Sequence

Protein


Download         Length: 187 a.a.        Molecular weight: 18960.66 Da        Isoelectric Point: 8.9648

>NTDB_id=1122866 EL216_RS08560 WP_085390949.1 1819985..1820548(-) (pilE) [Neisseria animaloris strain NCTC12228]
MKAIQKGFTLIELMIVVAIIGILAAIALPAYQDYTVRSKISEALISASAPKSLISEAFQTDGLTGVKNAAAAYNAKSKDE
KATKYVADMTIGGGNGNGAPGAADGVGVITVTLQDGNTAGGASFPTDVQGKTIILTPNVQKKALAAGAQGAIDWACTTTT
GENAKNRGLTATLNGTLPAKYAPSECR

Nucleotide


Download         Length: 564 bp        

>NTDB_id=1122866 EL216_RS08560 WP_085390949.1 1819985..1820548(-) (pilE) [Neisseria animaloris strain NCTC12228]
ATGAAAGCTATTCAAAAAGGTTTTACCCTAATCGAGTTGATGATCGTTGTAGCGATTATCGGTATTTTGGCTGCGATTGC
ATTGCCTGCTTACCAAGATTACACCGTTCGTTCCAAAATTTCTGAAGCCCTGATTTCTGCTTCTGCACCAAAGAGCCTGA
TCAGTGAAGCATTCCAAACAGATGGTTTAACCGGTGTGAAAAATGCTGCTGCTGCATATAATGCCAAATCTAAAGATGAA
AAAGCCACTAAATATGTCGCCGACATGACAATTGGTGGAGGTAATGGTAACGGTGCTCCTGGTGCAGCTGATGGTGTAGG
TGTTATTACTGTAACTTTGCAAGATGGGAATACCGCAGGTGGAGCTAGCTTCCCTACTGATGTACAAGGTAAAACTATTA
TTCTGACACCAAATGTACAGAAAAAAGCACTTGCCGCCGGTGCACAAGGTGCAATTGATTGGGCCTGTACCACAACAACT
GGTGAAAATGCCAAAAATAGAGGCTTAACCGCGACTCTCAATGGAACTTTACCTGCCAAATACGCCCCTTCTGAGTGCCG
TTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Pilin (bacterial filament) (Pilin)

(36-187)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

52.239

100

0.561
  pilA Ralstonia pseudosolanacearum GMI1000

42.105

100

0.428
  pilA2 Legionella pneumophila str. Paris

40.437

97.861

0.396
  pilA2 Legionella pneumophila strain ERS1305867

40.437

97.861

0.396
  comP Acinetobacter baylyi ADP1

38.83

100

0.39
  pilA Pseudomonas aeruginosa PAK

41.379

93.048

0.385

Multiple sequence alignment