Detailed information NTG1122610

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: EL220_RS04330
• Genome accession: NZ_LR134388
• Coordinates: 981151..982215 (+)
• Length: 354 a.a.
• NCBI ID: WP_027270596.1
• Uniprot ID: A0A0W0YC17
• Organism: Legionella sainthelensi strain NCTC11988
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 976151..987215

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL220_RS04320 (NCTC11988_00901)	-	976898..979263 (-)	2366	Protein_864	penicillin-binding protein 1A	-
EL220_RS04325 (NCTC11988_00902)	-	979490..980914 (-)	1425	WP_027270595.1	hypothetical protein	-
EL220_RS04330 (NCTC11988_00903)	pilM	981151..982215 (+)	1065	WP_027270596.1	type IV pilus assembly protein PilM	Machinery gene
EL220_RS04335 (NCTC11988_00904)	pilN	982220..982783 (+)	564	WP_027270597.1	PilN domain-containing protein	Machinery gene
EL220_RS04340 (NCTC11988_00905)	pilO	982780..983379 (+)	600	WP_027270598.1	type 4a pilus biogenesis protein PilO	Machinery gene
EL220_RS04345 (NCTC11988_00906)	pilP	983376..983882 (+)	507	WP_027270599.1	pilus assembly protein PilP	Machinery gene
EL220_RS04350 (NCTC11988_00907)	pilQ	983891..985996 (+)	2106	WP_027270600.1	type IV pilus secretin PilQ	Machinery gene
EL220_RS04355 (NCTC11988_00908)	aroK	986320..986847 (+)	528	WP_003632469.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38987.04 Da
• Isoelectric Point: 4.8891

>NTDB_id=1122610 EL220_RS04330 WP_027270596.1 981151..982215(+) (pilM) [Legionella sainthelensi strain NCTC11988]
MLQLLQPQHRSTLGIDITSTAVKILEISGKNGDLVIENYGRELLPPNAMDGNVIKDLDAVSQCVKKIVDRLHTPCKQAAL
AVPDAAIISKVVQINEGLNDEEMEELIATEADKYIPYPIEEINLDFEVLGPYPKNPHLLDVLIVASRAENVNQRVGIATA
AGLEAVVVDVESYAVERAAQRIVSDLPGLGKEKTIAIIDIGACYTHLFVLHKMKLVYSREEKFGDKQLIESIAEHYKITL
EEATVARDKKQLPSNYETEVLEPFKENILLQIKRTLQFFYSTNPEGEVDHILLAGGLARLPGLVTLVKDRLGKAAAIANP
LSYMTPSKKIHLDDINNDAPALMVACGLALRNVE

Nucleotide

• Length: 1065 bp

>NTDB_id=1122610 EL220_RS04330 WP_027270596.1 981151..982215(+) (pilM) [Legionella sainthelensi strain NCTC11988]
ATGCTTCAATTATTGCAACCCCAGCATCGTTCAACTCTTGGAATAGACATAACATCTACAGCAGTCAAAATATTAGAAAT
TTCAGGTAAGAATGGTGATCTTGTTATTGAGAATTACGGACGTGAACTATTGCCACCCAATGCAATGGATGGAAATGTTA
TCAAGGATCTCGATGCAGTCTCTCAATGTGTTAAAAAAATAGTTGACCGATTGCATACTCCGTGTAAGCAAGCTGCATTG
GCTGTGCCAGATGCGGCGATCATTAGTAAAGTAGTGCAAATTAATGAAGGCTTAAATGATGAGGAGATGGAGGAGCTTAT
CGCGACTGAGGCTGATAAATATATTCCTTATCCAATTGAAGAAATTAATCTAGATTTCGAGGTTTTGGGACCCTATCCGA
AAAACCCACATTTACTTGATGTGCTTATCGTTGCTTCGCGAGCAGAAAATGTCAACCAGCGTGTTGGAATAGCTACGGCT
GCGGGTTTAGAGGCTGTAGTAGTAGATGTTGAATCTTACGCAGTGGAGCGAGCAGCACAACGAATAGTTTCAGATTTACC
AGGACTTGGTAAAGAAAAGACAATTGCTATTATTGATATTGGGGCATGTTATACTCATTTATTCGTATTGCATAAAATGA
AATTAGTTTATTCTCGAGAAGAAAAATTTGGTGATAAGCAATTAATTGAATCAATTGCAGAGCACTATAAAATAACCTTA
GAGGAGGCAACAGTTGCTAGGGATAAAAAGCAATTACCTTCTAATTATGAAACAGAAGTTTTAGAGCCTTTTAAAGAAAA
TATCTTGTTGCAGATTAAGAGGACGTTGCAGTTTTTTTATTCTACAAACCCAGAAGGAGAGGTTGATCATATTCTATTGG
CTGGGGGATTAGCAAGATTACCAGGCTTAGTTACTCTAGTTAAAGACCGATTAGGAAAAGCAGCTGCTATCGCTAATCCA
CTTTCATATATGACCCCAAGTAAAAAAATCCATTTAGATGACATAAATAATGATGCGCCCGCACTAATGGTTGCTTGTGG
TTTAGCATTAAGAAATGTAGAGTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: A0A0W0YC17

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	65.819	100	0.658
pilM	Acinetobacter baumannii D1279779	42.655	100	0.427
comM	Acinetobacter nosocomialis M2	42.655	100	0.427
comM	Acinetobacter baylyi ADP1	40.113	100	0.401