Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   EL220_RS04335 Genome accession   NZ_LR134388
Coordinates   982220..982783 (+) Length   187 a.a.
NCBI ID   WP_027270597.1    Uniprot ID   -
Organism   Legionella sainthelensi strain NCTC11988     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 977220..987783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL220_RS04325 (NCTC11988_00902) - 979490..980914 (-) 1425 WP_027270595.1 hypothetical protein -
  EL220_RS04330 (NCTC11988_00903) pilM 981151..982215 (+) 1065 WP_027270596.1 type IV pilus assembly protein PilM Machinery gene
  EL220_RS04335 (NCTC11988_00904) pilN 982220..982783 (+) 564 WP_027270597.1 PilN domain-containing protein Machinery gene
  EL220_RS04340 (NCTC11988_00905) pilO 982780..983379 (+) 600 WP_027270598.1 type 4a pilus biogenesis protein PilO Machinery gene
  EL220_RS04345 (NCTC11988_00906) pilP 983376..983882 (+) 507 WP_027270599.1 pilus assembly protein PilP Machinery gene
  EL220_RS04350 (NCTC11988_00907) pilQ 983891..985996 (+) 2106 WP_027270600.1 type IV pilus secretin PilQ Machinery gene
  EL220_RS04355 (NCTC11988_00908) aroK 986320..986847 (+) 528 WP_003632469.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 187 a.a.        Molecular weight: 21922.63 Da        Isoelectric Point: 7.2778

>NTDB_id=1122611 EL220_RS04335 WP_027270597.1 982220..982783(+) (pilN) [Legionella sainthelensi strain NCTC11988]
MTQINLLPWREQKREQEKKVFMVLFCIVIVISIFVVFLINTYASDLVSNQLVRNQMLQDEIAAMNNQLNEIKSLKKTREL
FISRMFIVQHLQSTRTLMVHLFDELINVMPSGIYLTKVEGKNDILTVTGYTESNTYVSILMKNIENNEWIHSPVLSEIKK
EDRQEPANNEFKLNFVLTPQFQLGIIR

Nucleotide


Download         Length: 564 bp        

>NTDB_id=1122611 EL220_RS04335 WP_027270597.1 982220..982783(+) (pilN) [Legionella sainthelensi strain NCTC11988]
ATGACACAAATTAATCTTCTTCCTTGGCGTGAACAAAAGCGGGAGCAAGAAAAAAAGGTGTTCATGGTTTTATTTTGCAT
AGTTATAGTAATTTCAATATTTGTCGTGTTTTTAATTAATACTTATGCGTCTGATTTAGTGAGTAATCAACTGGTTCGAA
ATCAGATGCTGCAAGATGAAATAGCGGCTATGAATAACCAGCTAAACGAAATTAAAAGCTTGAAAAAAACTAGAGAGCTG
TTCATCTCTAGAATGTTTATAGTTCAGCATTTACAATCAACTAGGACATTAATGGTTCATTTATTTGATGAGTTAATTAA
TGTAATGCCTTCTGGCATTTATTTAACAAAGGTAGAAGGGAAAAATGATATTCTCACTGTCACTGGGTATACTGAATCGA
ACACCTACGTTTCAATTTTAATGAAAAATATAGAGAATAATGAATGGATACATTCTCCTGTTCTGAGTGAAATTAAGAAG
GAAGACAGGCAGGAGCCTGCTAATAATGAATTTAAGTTAAATTTTGTACTTACCCCTCAATTCCAGCTGGGAATTATACG
ATGA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-175)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

62.295

97.861

0.61

Multiple sequence alignment