Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EL249_RS01045 Genome accession   NZ_LR134378
Coordinates   248196..248711 (-) Length   171 a.a.
NCBI ID   WP_005674919.1    Uniprot ID   E7S0K0
Organism   Lautropia mirabilis strain NCTC12852     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 232920..248027 248196..248711 flank 169


Gene organization within MGE regions


Location: 232920..248711
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL249_RS00975 (NCTC12852_00192) - 232920..233789 (-) 870 WP_040532121.1 ABC transporter permease -
  EL249_RS00980 (NCTC12852_00193) - 233925..234875 (-) 951 WP_005674933.1 ABC transporter permease -
  EL249_RS00985 (NCTC12852_00194) - 234894..236432 (-) 1539 WP_005674932.1 ABC transporter substrate-binding protein -
  EL249_RS00990 (NCTC12852_00195) - 236772..237314 (+) 543 WP_005674930.1 cupin domain-containing protein -
  EL249_RS00995 (NCTC12852_00196) mntA 237458..237898 (+) 441 WP_005674929.1 type VII toxin-antitoxin system MntA family adenylyltransferase antitoxin -
  EL249_RS01000 (NCTC12852_00197) - 238222..238911 (+) 690 WP_040530225.1 hypothetical protein -
  EL249_RS01005 (NCTC12852_00198) - 239140..239409 (+) 270 WP_040530224.1 BrnT family toxin -
  EL249_RS01010 (NCTC12852_00199) - 239393..239728 (+) 336 WP_005674927.1 BrnA antitoxin family protein -
  EL249_RS01015 (NCTC12852_00200) - 239913..240854 (-) 942 WP_005674926.1 hypothetical protein -
  EL249_RS01020 (NCTC12852_00201) - 241070..241861 (-) 792 WP_005674924.1 hypothetical protein -
  EL249_RS01025 (NCTC12852_00202) - 242164..242898 (+) 735 WP_005674923.1 hypothetical protein -
  EL249_RS01030 (NCTC12852_00203) - 243356..245059 (+) 1704 WP_040530219.1 DUF262 domain-containing protein -
  EL249_RS01035 (NCTC12852_00204) - 245813..247078 (+) 1266 WP_083799583.1 DNA-processing protein DprA -
  EL249_RS01040 (NCTC12852_00205) - 247167..247862 (-) 696 WP_126348025.1 ComF family protein -
  EL249_RS01045 (NCTC12852_00206) pilA 248196..248711 (-) 516 WP_005674919.1 pilin Machinery gene

Sequence

Protein


Download         Length: 171 a.a.        Molecular weight: 17834.57 Da        Isoelectric Point: 9.0245

>NTDB_id=1122460 EL249_RS01045 WP_005674919.1 248196..248711(-) (pilA) [Lautropia mirabilis strain NCTC12852]
MMLKQVQKGFTLIELMIVVAIIGILAAVAIPAYQDYTIRARVSEGLSLAAAAKLNVAELAAGGQNDAVTTGYGTGYNPPS
KSTNVDQVTITPATGEISITYRTRVAAKTENLLVLTPFAGANGLPDGTKAFSPVQDAIQWRCRANGVASPVTITGALSPT
LPTRFAPAECR

Nucleotide


Download         Length: 516 bp        

>NTDB_id=1122460 EL249_RS01045 WP_005674919.1 248196..248711(-) (pilA) [Lautropia mirabilis strain NCTC12852]
ATGATGCTGAAACAGGTTCAAAAAGGTTTCACCCTGATCGAGCTGATGATCGTCGTGGCGATCATCGGTATTCTGGCCGC
CGTGGCCATCCCGGCTTACCAGGACTACACGATCCGCGCTCGCGTGTCGGAAGGCCTGTCGCTGGCTGCTGCTGCCAAGC
TGAACGTGGCAGAGCTTGCTGCTGGTGGTCAGAATGATGCCGTGACGACCGGGTATGGCACGGGCTATAACCCGCCCAGC
AAGTCCACCAACGTTGATCAGGTGACGATTACGCCGGCTACCGGCGAAATCTCGATCACGTATCGTACTCGTGTCGCCGC
CAAGACCGAGAACCTTCTGGTTCTGACTCCGTTTGCTGGTGCGAATGGTCTGCCGGACGGTACTAAAGCATTCAGCCCGG
TTCAGGATGCCATCCAATGGCGTTGCCGTGCTAACGGTGTAGCGTCTCCTGTTACGATTACCGGAGCCCTGTCTCCGACG
CTGCCGACGCGCTTTGCCCCTGCCGAGTGCCGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-29)

Pilin (bacterial filament) (Pilin)

(39-171)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E7S0K0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

49.425

100

0.503
  pilA2 Legionella pneumophila str. Paris

48.193

97.076

0.468
  pilA2 Legionella pneumophila strain ERS1305867

47.59

97.076

0.462
  comP Acinetobacter baylyi ADP1

39.08

100

0.398
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.555

100

0.386
  pilE Neisseria gonorrhoeae strain FA1090

35.196

100

0.368

Multiple sequence alignment