Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EL199_RS05795 Genome accession   NZ_LR134365
Coordinates   1177990..1179684 (+) Length   564 a.a.
NCBI ID   WP_004139636.1    Uniprot ID   C8N7I7
Organism   Cardiobacterium hominis strain NCTC10426     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1172990..1184684
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL199_RS05760 (NCTC10426_01117) fni 1173193..1174215 (-) 1023 WP_004139621.1 type 2 isopentenyl-diphosphate Delta-isomerase -
  EL199_RS05765 (NCTC10426_01118) - 1174212..1175258 (-) 1047 WP_004139623.1 hydroxymethylglutaryl-CoA reductase -
  EL199_RS05775 (NCTC10426_01120) rpsI 1175612..1176001 (-) 390 WP_004139624.1 30S ribosomal protein S9 -
  EL199_RS05780 (NCTC10426_01121) rplM 1176011..1176442 (-) 432 WP_004139625.1 50S ribosomal protein L13 -
  EL199_RS05785 - 1176670..1177338 (-) 669 WP_004139626.1 DNA repair protein RecO -
  EL199_RS05790 (NCTC10426_01123) - 1177403..1177993 (+) 591 WP_004139634.1 aminodeoxychorismate/anthranilate synthase component II -
  EL199_RS05795 (NCTC10426_01124) pilB 1177990..1179684 (+) 1695 WP_004139636.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL199_RS05800 (NCTC10426_01125) - 1179702..1180919 (+) 1218 WP_004139638.1 type II secretion system F family protein -
  EL199_RS05805 (NCTC10426_01126) - 1180919..1181800 (+) 882 WP_050768035.1 A24 family peptidase -
  EL199_RS05810 (NCTC10426_01127) coaE 1181802..1182425 (+) 624 WP_004139640.1 dephospho-CoA kinase -
  EL199_RS05815 (NCTC10426_01128) - 1182474..1182938 (-) 465 WP_004139641.1 S-ribosylhomocysteine lyase -
  EL199_RS05820 (NCTC10426_01129) yegQ 1183095..1184435 (+) 1341 WP_004139642.1 tRNA 5-hydroxyuridine modification protein YegQ -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62650.54 Da        Isoelectric Point: 5.3073

>NTDB_id=1122317 EL199_RS05795 WP_004139636.1 1177990..1179684(+) (pilB) [Cardiobacterium hominis strain NCTC10426]
MSDYDPLISYMVQHKICTPEQIREVQISAQKKRGSFLQQLLSDGLIDGSRLSQINREVYNLPQVMLRELDVRRELTAQFP
ETVVRKHMALPIYANGGRLFIATFDPSNRIMLEEFKYQGKFTSVEPIIADIGVIEALIEDHYAGVGGMESVFEDENQEQD
LDAISEALGNIGANEEEAPVVRFVSGMLMDAIRTGASDLHFEPYENTYRVRFRRDGVLQEVAAPPPSIANRITARLKVMA
NLDIAEKRVPQDGRIKLQVSETKAIDFRVSSLPTLWGEKIVLRILDSSAAKLNIDVLGFEPDQKQHYLDAISKPQGLVLV
TGPTGSGKTVSLYTGLSILNKPTVNISTAEDPVEINLPGINQVNVNTKTGLDFAAALKAFLRQDPDIIMVGEIRDITTGE
IAVKAAQTGHLVLSTLHTNDVPQTIARLVNIGIPSYNIAASVNLIMAQRLTRRLCSHCKTRDRKHSHDELIALGFAEDEI
DDLRIYAPKGCERCSYQGYRGRAGIYQVVPISESLSEMILHNAAAADIAEQCQKEGYWDLRQSALNKVRQGLTSIEEILR
VTTD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=1122317 EL199_RS05795 WP_004139636.1 1177990..1179684(+) (pilB) [Cardiobacterium hominis strain NCTC10426]
ATGAGTGATTATGATCCTTTAATCAGCTATATGGTACAGCACAAAATATGCACACCCGAGCAAATTAGGGAAGTGCAAAT
TAGTGCGCAGAAAAAACGGGGCAGTTTTTTACAACAATTACTGAGTGATGGTTTGATAGACGGTTCACGACTATCGCAAA
TTAATCGAGAAGTTTACAACTTGCCACAAGTTATGTTGCGCGAATTGGATGTTAGACGTGAATTAACTGCGCAATTCCCG
GAAACCGTTGTGCGAAAACACATGGCGCTTCCTATCTATGCTAATGGTGGACGACTATTTATTGCGACGTTTGATCCTTC
AAACCGTATTATGCTGGAGGAGTTTAAATATCAAGGTAAATTTACTTCTGTTGAACCAATTATAGCGGATATAGGTGTAA
TAGAAGCACTTATAGAGGATCACTACGCTGGTGTAGGCGGGATGGAGAGTGTTTTTGAGGATGAAAATCAAGAACAAGAT
CTGGATGCCATCAGTGAGGCGCTAGGTAATATTGGTGCAAATGAAGAAGAAGCGCCTGTTGTTCGTTTTGTTTCGGGCAT
GCTAATGGATGCTATTCGTACTGGGGCTTCGGATTTGCACTTTGAGCCTTATGAAAATACTTATCGGGTGCGATTCCGCC
GAGACGGTGTCTTGCAAGAAGTTGCAGCACCTCCTCCCAGTATTGCAAACCGTATTACTGCACGCTTAAAAGTTATGGCA
AATTTGGATATTGCAGAGAAGCGTGTTCCTCAGGATGGGCGTATTAAACTTCAGGTTTCTGAAACAAAAGCTATCGATTT
CCGGGTTAGCTCATTGCCAACGTTATGGGGAGAAAAAATCGTACTACGTATTCTCGATAGTTCAGCAGCTAAACTAAATA
TCGATGTTCTTGGCTTTGAACCAGATCAGAAGCAGCATTATCTCGATGCTATTAGTAAACCCCAAGGTCTAGTATTGGTT
ACCGGCCCTACTGGCTCAGGTAAAACCGTTTCTCTGTATACCGGGCTATCTATTCTGAATAAACCAACTGTTAATATTTC
TACTGCTGAAGATCCTGTGGAAATAAACTTACCCGGTATTAACCAGGTTAACGTAAATACCAAGACAGGATTGGATTTTG
CAGCCGCGCTAAAAGCCTTTTTGCGTCAGGATCCTGATATCATCATGGTTGGGGAGATTCGTGATATCACGACAGGTGAA
ATTGCCGTAAAAGCGGCGCAAACGGGGCACTTGGTTTTATCTACATTGCACACTAACGATGTACCACAAACGATTGCACG
TTTGGTGAATATCGGGATTCCCTCCTATAACATTGCTGCTAGTGTAAATTTAATCATGGCACAGCGATTGACGCGCCGCT
TGTGTAGCCACTGCAAAACGCGTGACCGAAAACATAGTCATGATGAGTTGATCGCCTTAGGCTTTGCCGAAGATGAGATT
GATGATCTACGGATTTATGCTCCTAAAGGGTGTGAACGTTGCAGTTATCAAGGATATCGTGGTCGCGCGGGTATTTACCA
AGTTGTGCCGATCTCTGAATCGTTGTCAGAAATGATTTTGCATAATGCAGCGGCAGCAGATATTGCTGAACAATGCCAAA
AAGAAGGCTATTGGGATTTACGTCAATCTGCATTGAATAAAGTACGTCAAGGTCTGACCAGTATTGAAGAGATCTTGCGT
GTAACAACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(187-453)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C8N7I7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.491

88.83

0.484
  pilF Neisseria gonorrhoeae MS11

48.05

100

0.481
  pilB Acinetobacter baylyi ADP1

47.535

100

0.479
  pilB Legionella pneumophila strain ERS1305867

46.384

100

0.466
  pilB Vibrio cholerae strain A1552

47.091

97.518

0.459
  pilB Vibrio campbellii strain DS40M4

47.191

94.681

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

47.18

94.326

0.445
  pilF Thermus thermophilus HB27

36.945

99.823

0.369
  pilB Haemophilus influenzae 86-028NP

38.71

93.44

0.362
  pilB/pilB1 Synechocystis sp. PCC 6803

38.931

92.908

0.362

Multiple sequence alignment