Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EL332_RS20900 Genome accession   NZ_LR134334
Coordinates   4682291..4684363 (+) Length   690 a.a.
NCBI ID   WP_124323333.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain NCTC7357     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4677291..4689363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL332_RS20880 (NCTC7357_04170) pilM 4679521..4680573 (+) 1053 WP_124323331.1 pilus assembly protein PilM Machinery gene
  EL332_RS20885 (NCTC7357_04171) - 4680573..4681139 (+) 567 WP_025808191.1 PilN domain-containing protein -
  EL332_RS20890 (NCTC7357_04172) - 4681136..4681753 (+) 618 WP_063427904.1 type 4a pilus biogenesis protein PilO -
  EL332_RS20895 (NCTC7357_04173) - 4681750..4682277 (+) 528 WP_124323332.1 pilus assembly protein PilP -
  EL332_RS20900 (NCTC7357_04174) pilQ 4682291..4684363 (+) 2073 WP_124323333.1 type IV pilus secretin PilQ Machinery gene
  EL332_RS20905 (NCTC7357_04175) aroK 4684368..4684886 (+) 519 WP_009041824.1 shikimate kinase AroK -
  EL332_RS20910 (NCTC7357_04176) aroB 4685035..4686135 (+) 1101 WP_025808185.1 3-dehydroquinate synthase -
  EL332_RS20915 (NCTC7357_04177) - 4686146..4687735 (+) 1590 WP_124323334.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74607.77 Da        Isoelectric Point: 5.2947

>NTDB_id=1121783 EL332_RS20900 WP_124323333.1 4682291..4684363(+) (pilQ) [Pseudomonas chlororaphis strain NCTC7357]
MNRIFSAFGIALWMALLSPMAMAASLKALDVAALPGDRLELKLTFDGPVPEPRGYTTDQPARIALDLPGVVSQLANKSRD
LGNGNARSVTVVESGQRTRVVVNLVALAPYSTRVAGNQLFVVIGQGATAAQSALGLPAVASGPAKAAASVGRSIRSVDFQ
RGDQGEGNVLIDLSDSGMSPDIQERDGKLVLTFAKARLPEPLRVRLDVKDFATPVQFVSARAESGKAIITIEPGGTFDYS
TYQTDNKLTVSVRPMTSGDLQKRNVERSAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGTNG
SSTIGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
AASSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=1121783 EL332_RS20900 WP_124323333.1 4682291..4684363(+) (pilQ) [Pseudomonas chlororaphis strain NCTC7357]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGCTGCTTTCACCGATGGCCATGGCGGCCAGCCTCAA
GGCCCTGGATGTCGCGGCATTGCCGGGGGATCGCCTGGAGTTGAAGTTGACCTTCGATGGGCCGGTGCCCGAGCCGCGGG
GTTATACCACCGACCAGCCGGCGCGAATCGCCCTCGATCTGCCGGGTGTCGTCAGCCAGCTGGCGAACAAGAGCCGCGAT
CTGGGCAATGGCAATGCGCGCAGCGTGACGGTGGTCGAATCGGGGCAGCGTACCCGGGTGGTGGTCAACCTGGTCGCGCT
GGCGCCCTACAGCACCCGGGTCGCCGGCAATCAGCTGTTTGTGGTGATCGGCCAGGGGGCGACGGCGGCGCAGAGTGCAC
TGGGGCTGCCTGCTGTCGCGTCCGGGCCGGCCAAGGCCGCGGCTTCCGTAGGACGGTCGATTCGCAGCGTGGATTTCCAG
CGCGGCGATCAGGGCGAGGGCAATGTGCTGATCGACCTCTCCGATTCGGGAATGAGCCCGGATATACAGGAACGCGACGG
CAAGCTCGTCCTCACCTTCGCCAAGGCCCGCCTGCCAGAGCCCTTGCGGGTCAGGCTCGACGTCAAGGATTTCGCCACCC
CCGTGCAGTTCGTCAGTGCCCGGGCGGAGTCCGGCAAGGCCATCATCACCATCGAGCCTGGCGGCACCTTCGATTACTCG
ACCTACCAGACGGACAACAAGCTTACGGTGAGTGTCAGGCCGATGACTTCGGGCGATCTGCAGAAGCGCAACGTCGAGCG
CTCGGCCTATAACGGCGAAAAGCTCTCGCTGAACTTCCAGGACATCGAGGTCCGCTCGGTGCTGCAACTGATCGCCGACT
TCACCAACCTCAACCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGCCTGCAGAATGTGCCGTGGGACCAG
GCCCTCGATCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGTGGGCAATGTGTTGCTGGTGGCGCCGGCCGATGA
AATCGCCGCTCGCGAACGCCAGGAGCTGGAGTCACAGAAGCAGATAGCCGACTTGGAGCCGCTGCGGCGCGAACTGCTAC
AGGTCAACTACGCCAAGGCCGCGGAAATCGCCAAGTTGTTCCAGTCGGTCACCCGCGCCGAGGACAAGTCCGATGAGCGT
GGTTCGATCACCGTCGATGAACGGACCAACAACATCATTGCCTACCAGACCCGGGAGCGCCTGGACGAGCTGCGGCGGAT
TGTCAGCCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAAGCGCGGATCGTCGAGGCCAACGTCGACTACGACAAAA
GCCTGGGCGTGCGCTGGGGCGGTTCGATCCAGAACCAGGGCAACTGGAATGCCTCGGGGGTCAGCAACGGCACCAACGGC
TCTTCCACCATAGGCACGCCGGGCAGTACCAGCAGCAATTCACCCTTCGTCGACCTGGGCACCACCGCCAATACCTCCGG
CCTGGGTATCGCCTTCATCACCGACAACGTGTTGCTGGACCTTGAACTGACGGCCATGGAAAAGACCGGTAACGGTGAAA
TCGTCTCCCAGCCCAAGGTCGTCACGTCCGACAAGGAGACGGCGAAGATCCTCAAGGGCACGGAAATCCCTTATCAGGAA
GCGGCCTCCAGCGGAGCGACCTCGGTGTCCTTCAAGGAGGCCTCGCTGTCCCTGGAGGTGACGCCGCAAATTACCCCGGA
TAATCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAGGTCCAGGACGTGCCGCCGATCA
AGAAAAACGAGGTCAACGCCAAGGTCCTGATCAACGACGGAGAAACCATCGTTATCGGTGGGGTTTTCTCCAATACGCAA
AGCAAGGTTGTAGATAAGGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGACTTTTCCGGCGTGACGTGGTGTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Secretin and TonB N terminus short domain (STN)

(298-344)

AMIN domain (AMIN)

(40-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.35

100

0.729
  comQ Acinetobacter baylyi ADP1

40.11

100

0.423
  pilQ Acinetobacter baumannii D1279779

38.165

100

0.416
  pilQ Acinetobacter baumannii strain A118

38.165

100

0.416
  pilQ Legionella pneumophila strain Lp02

37.89

97.536

0.37
  pilQ Legionella pneumophila strain ERS1305867

37.967

96.957

0.368

Multiple sequence alignment