Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   EL332_RS20880 Genome accession   NZ_LR134334
Coordinates   4679521..4680573 (+) Length   350 a.a.
NCBI ID   WP_124323331.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain NCTC7357     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4674521..4685573
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL332_RS20870 (NCTC7357_04168) - 4675433..4676701 (+) 1269 WP_025808196.1 malic enzyme-like NAD(P)-binding protein -
  EL332_RS20875 (NCTC7357_04169) - 4676869..4679304 (-) 2436 WP_164486060.1 penicillin-binding protein 1A -
  EL332_RS20880 (NCTC7357_04170) pilM 4679521..4680573 (+) 1053 WP_124323331.1 pilus assembly protein PilM Machinery gene
  EL332_RS20885 (NCTC7357_04171) - 4680573..4681139 (+) 567 WP_025808191.1 PilN domain-containing protein -
  EL332_RS20890 (NCTC7357_04172) - 4681136..4681753 (+) 618 WP_063427904.1 type 4a pilus biogenesis protein PilO -
  EL332_RS20895 (NCTC7357_04173) - 4681750..4682277 (+) 528 WP_124323332.1 pilus assembly protein PilP -
  EL332_RS20900 (NCTC7357_04174) pilQ 4682291..4684363 (+) 2073 WP_124323333.1 type IV pilus secretin PilQ Machinery gene
  EL332_RS20905 (NCTC7357_04175) aroK 4684368..4684886 (+) 519 WP_009041824.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37231.70 Da        Isoelectric Point: 5.1221

>NTDB_id=1121782 EL332_RS20880 WP_124323331.1 4679521..4680573(+) (pilM) [Pseudomonas chlororaphis strain NCTC7357]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGNRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLSDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEM
AGLTARVVDLEACALERSFALAAGSSVARTVALIDIGATLTTLSVVHEGQIIYSREQLFGGHQLTEHIQRRYRLSKEEAG
LVKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQLFQASGPQRQVDSILLAGGSASVPGLAPLIEQRLSLPVLIANPFAGM
SIGGKVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=1121782 EL332_RS20880 WP_124323331.1 4679521..4680573(+) (pilM) [Pseudomonas chlororaphis strain NCTC7357]
GTGCTAGGACTCTTCAGCAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAGTT
GAGCCGGGTCGGCAACCGCTTTCGGGTCGAGGCCTATGGGGTGGAGCCGTTGCCGGCCAACGCCGTGGTCGAGAAAAACA
TCGTCGAGCCCGAAGGGGTGGGGCATGCCCTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGAGCGAAGCACGCGGCGCTG
GCGATGGCCGGTTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGACTTTCCGATGATGATCGGGAGAGCCAGCT
CAAGGTCGACGCCGACCAGTACATTCCCTATCCACTGGACGAGGCCGCCATCGATTTCGACGTCCTCGGCTATTCGGCGC
GCAACCCCGAGCGGGTCGATGTGCTGTTGGCGGCTTGCCGCAAGGAACACATCGAAGTTCGCGAGGCGGCGCTCGAAATG
GCGGGATTGACGGCGCGGGTGGTGGACCTGGAAGCCTGCGCGCTGGAGCGTTCGTTCGCCCTGGCGGCCGGGTCATCGGT
TGCCCGGACCGTGGCGCTGATCGATATCGGCGCCACCCTGACCACCCTGAGCGTTGTGCATGAAGGGCAGATCATCTATT
CCCGCGAGCAGTTGTTTGGCGGGCATCAGTTGACCGAGCACATCCAGCGTCGTTACAGGCTGTCGAAAGAAGAGGCCGGC
CTGGTGAAAAAGCAGGGCGGCCTGCCGGACGACTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTGGTACAGCA
GGTTTCGCGCTCGTTGCAGCTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGATTCCATCCTGTTGGCGGGGGGTAGCG
CCTCGGTTCCAGGGCTTGCACCGTTGATCGAGCAACGCCTGAGCTTGCCCGTGCTGATCGCCAACCCCTTCGCTGGCATG
AGCATCGGCGGCAAGGTCGATGCCGCGGCCCTGGCCAGCGAGGCCCCGTCCCTGATGATCGCTTGTGGGCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

54.261

100

0.546
  comM Acinetobacter nosocomialis M2

53.977

100

0.543
  comM Acinetobacter baylyi ADP1

51.42

100

0.517
  pilM Legionella pneumophila strain ERS1305867

44.915

100

0.454

Multiple sequence alignment