Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EL185_RS19355 Genome accession   NZ_LR134321
Coordinates   4461078..4462790 (+) Length   570 a.a.
NCBI ID   WP_126492938.1    Uniprot ID   -
Organism   Shewanella baltica strain NCTC10737     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4456078..4467790
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL185_RS19330 (NCTC10737_03892) pdhR 4456121..4456873 (-) 753 WP_126492930.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  EL185_RS19335 (NCTC10737_03893) ampE 4457190..4458041 (-) 852 WP_126492932.1 beta-lactamase regulator AmpE -
  EL185_RS19340 (NCTC10737_03894) ampD 4458111..4458674 (-) 564 WP_126492934.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EL185_RS19345 (NCTC10737_03895) nadC 4458945..4459832 (+) 888 WP_126492936.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EL185_RS21975 (NCTC10737_03896) - 4460558..4461013 (+) 456 WP_269471924.1 pilin -
  EL185_RS19355 (NCTC10737_03897) pilB 4461078..4462790 (+) 1713 WP_126492938.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL185_RS19360 (NCTC10737_03898) pilC 4462919..4464184 (+) 1266 WP_126492940.1 type II secretion system F family protein Machinery gene
  EL185_RS19365 (NCTC10737_03899) pilD 4464228..4465154 (+) 927 WP_126492942.1 prepilin peptidase Machinery gene
  EL185_RS19370 (NCTC10737_03900) coaE 4465194..4465811 (+) 618 WP_126492943.1 dephospho-CoA kinase -
  EL185_RS19375 (NCTC10737_03901) zapD 4465804..4466538 (+) 735 WP_126492945.1 cell division protein ZapD -
  EL185_RS19380 (NCTC10737_03902) yacG 4466612..4466821 (+) 210 WP_006083355.1 DNA gyrase inhibitor YacG -
  EL185_RS19385 (NCTC10737_03903) mutT 4466898..4467290 (+) 393 WP_126492947.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63026.36 Da        Isoelectric Point: 5.8421

>NTDB_id=1121464 EL185_RS19355 WP_126492938.1 4461078..4462790(+) (pilB) [Shewanella baltica strain NCTC10737]
MPTTGLHLGLSTLFIRKGLLSEEQMANAITKSRQNKHGLVSTLVQEKLISARAIAELCYEEYGTPLLDLNEFDLASIPED
FLNKKLIEKHRCLPLFKRGNRLYIGTSDPTNIAALEDFQFSAGLHAEAILVEEDKLAKALEKILEEDITGLDISSMDEAA
LAGIEITDTDRRNEEPTGDSSDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVSLASRLSA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEPDQEKLYLEMLAKP
QGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCSECKQAEVIPEHELLRLGF
DQTQITQGFTTFKPVGCEHCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIASQAKAQGMRDLRQSGLLKVIHGVTSI
AEVNRVTSFD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1121464 EL185_RS19355 WP_126492938.1 4461078..4462790(+) (pilB) [Shewanella baltica strain NCTC10737]
ATGCCTACCACTGGTCTTCATTTAGGTTTATCAACCCTTTTTATCCGTAAAGGCCTGCTGAGTGAAGAGCAGATGGCTAA
CGCCATCACAAAATCCCGTCAGAATAAACATGGACTTGTTAGCACCTTAGTCCAAGAAAAACTCATTTCAGCCCGTGCCA
TTGCCGAGCTGTGTTATGAAGAATATGGCACGCCATTATTAGATTTGAATGAATTTGATCTTGCCTCCATTCCAGAAGAT
TTCCTCAATAAAAAGCTGATTGAAAAACATCGCTGTTTACCCTTGTTTAAACGCGGTAATCGCCTCTATATAGGCACATC
CGACCCAACCAATATTGCCGCCCTCGAAGATTTCCAATTTAGTGCTGGCCTACACGCCGAAGCCATTTTGGTAGAAGAAG
ATAAACTCGCTAAGGCGCTGGAAAAAATCCTCGAAGAAGATATCACCGGGCTCGATATCAGTAGCATGGATGAGGCGGCG
CTGGCGGGGATTGAAATTACCGATACAGATAGGCGCAACGAAGAACCCACAGGCGATAGCAGCGACGATGCACCTATCGT
TATTTATATCAATAAAATTCTAACCGATGCCATCCGCAAAGGCGCGTCGGATTTACATTTTGAACCCTATGAAAAACGCT
ACCGTATTCGTTTTCGCATTGACGGTATTCTCCACGAAGTATCGGAACCGCCAGTTAGCCTTGCCAGCCGTTTATCAGCG
CGGTTAAAAGTGATGTCGAAACTCGATATTGCCGAACGGCGTGTGCCACAAGATGGCCGCATTAAGATGAAACTTTCCCG
CACTAAGTCCATCGACTTTCGGGTTAGTACTCTGCCGACCCTGTGGGGTGAAAAGATTGTAATGCGGATTTTAGACTCAT
CCTCCGCACAATTAGGCATTGAAAAGCTCGGCTACGAGCCTGACCAAGAAAAGCTCTACCTTGAGATGCTAGCAAAACCC
CAAGGCATGATTTTAGTAACGGGGCCCACAGGTTCTGGTAAAACCGTCTCCCTCTACACTGGGTTAAATATTCTCAATAC
CGAAGAGCGTAATATATCTACCGCCGAAGATCCGGTGGAGATTAACCTCGAAGGCGTGAACCAAGTTCACATTAATTTAA
AGGCAGGCCTCACCTTCGCCTCTGCACTGCGTTCGTTCTTACGCCAAGATCCCGATGTGGTGATGGTGGGCGAAATTCGC
GATCTTGAAACTGCCGAGATAGCCATTAAAGCGGCACAAACGGGTCACTTAGTCTTATCGACCTTACACACAAACTCGGC
CGCCGAAACCTTAACCCGTTTAATCAACATGGGTGTGCCTGGCTACAACATAGCTAGCTCTGTGAATCTGATTATTGCCC
AGCGCTTAGCCCGTCGCCTCTGCTCTGAATGTAAACAAGCAGAAGTGATCCCCGAGCATGAATTACTGCGCCTAGGTTTT
GACCAAACACAAATTACTCAAGGCTTTACCACCTTTAAACCCGTTGGCTGCGAGCATTGCTCCGGCGGCTACAAAGGTCG
GGTCGGTATTTACGAAGTAATGAAGATGTCCGATGAAATCGCGCGCACTATTATGGAAGGCGGTAACTCATTACAAATCG
CCTCCCAAGCTAAAGCGCAAGGTATGCGAGATTTGCGTCAATCAGGATTATTAAAAGTTATCCACGGTGTAACGAGTATT
GCTGAAGTCAACCGAGTCACGAGTTTCGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.537

98.421

0.586
  pilB Acinetobacter baumannii D1279779

57.932

98.421

0.57
  pilB Legionella pneumophila strain ERS1305867

56.406

98.596

0.556
  pilB Vibrio cholerae strain A1552

53.298

98.421

0.525
  pilB Vibrio parahaemolyticus RIMD 2210633

51.142

99.825

0.511
  pilB Vibrio campbellii strain DS40M4

51.237

99.298

0.509
  pilF Neisseria gonorrhoeae MS11

47.527

99.298

0.472
  pilF Thermus thermophilus HB27

38.977

99.474

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.71

97.895

0.379

Multiple sequence alignment