Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EL257_RS01925 Genome accession   NZ_LR134318
Coordinates   423131..425209 (+) Length   692 a.a.
NCBI ID   WP_126359328.1    Uniprot ID   A0A448DKD5
Organism   Pseudomonas fluorescens strain NCTC9428     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 418131..430209
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL257_RS01905 (NCTC9428_00386) pilM 420343..421407 (+) 1065 WP_126359321.1 pilus assembly protein PilM Machinery gene
  EL257_RS01910 (NCTC9428_00387) - 421407..421973 (+) 567 WP_126359323.1 PilN domain-containing protein -
  EL257_RS01915 (NCTC9428_00388) pilO 421970..422593 (+) 624 WP_126359325.1 type 4a pilus biogenesis protein PilO -
  EL257_RS01920 (NCTC9428_00389) - 422590..423117 (+) 528 WP_126359326.1 pilus assembly protein PilP -
  EL257_RS01925 (NCTC9428_00390) pilQ 423131..425209 (+) 2079 WP_126359328.1 type IV pilus secretin PilQ Machinery gene
  EL257_RS01930 (NCTC9428_00391) aroK 425214..425732 (+) 519 WP_045122260.1 shikimate kinase AroK -
  EL257_RS01935 (NCTC9428_00392) aroB 425883..426983 (+) 1101 WP_126359330.1 3-dehydroquinate synthase -
  EL257_RS01940 (NCTC9428_00393) - 426993..428567 (+) 1575 WP_126359332.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 75105.64 Da        Isoelectric Point: 5.5570

>NTDB_id=1121314 EL257_RS01925 WP_126359328.1 423131..425209(+) (pilQ) [Pseudomonas fluorescens strain NCTC9428]
MNRIFSTLGFSLWIALMSPMVLAANLKTLDVAALPGDRIELKLSFDGPPPQPKGYTTESPARIALDLPGVASQLASKNVD
LGSGNARTATVVEAKDRTRLIVSLTQLTPYTTRVEGSTLFVVVGQGAPAAAPRPAVVPRAKPVAAAPVKAYVPTSRAIRG
VDFQRGTSGEGNVVIDLSDPTIAPDIQEHDGKIILAFARTQLPEKLRVRLDVKDFATPVQFVNAAVTADRTVITVEPSGT
YEYSTFQTDNKLTVSIRPMTLDDLQKRNADRQAYVGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNV
PWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAK
VDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNTSGVSN
GSSTTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=1121314 EL257_RS01925 WP_126359328.1 423131..425209(+) (pilQ) [Pseudomonas fluorescens strain NCTC9428]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGATGTCGCCGATGGTACTCGCGGCCAACCTGAA
GACGCTGGATGTCGCAGCGTTACCGGGGGATCGCATTGAATTGAAGCTGTCCTTTGACGGTCCGCCGCCGCAACCCAAGG
GCTACACCACCGAGTCGCCGGCGCGAATTGCGCTCGATCTGCCTGGGGTTGCCAGTCAGCTGGCGAGCAAAAATGTCGAT
CTGGGCAGCGGCAATGCCCGCACGGCCACGGTGGTTGAAGCAAAGGATCGAACGCGCCTGATCGTCAGTCTGACGCAGCT
GACGCCCTATACCACACGGGTTGAAGGCAGCACGCTGTTCGTGGTGGTCGGGCAGGGCGCGCCGGCTGCCGCACCGCGCC
CGGCCGTCGTGCCGCGCGCCAAGCCAGTCGCCGCAGCGCCGGTCAAGGCCTACGTGCCGACCAGTCGGGCCATTCGGGGC
GTCGACTTCCAGCGCGGCACCTCCGGCGAGGGCAACGTCGTCATCGACCTGTCCGACCCGACCATCGCCCCGGATATTCA
GGAGCATGACGGCAAGATCATCCTCGCTTTCGCCCGCACGCAACTGCCGGAAAAGCTGCGGGTGCGTCTCGACGTCAAGG
ATTTCGCAACCCCGGTGCAATTCGTCAATGCTGCTGTGACAGCCGATCGCACGGTCATTACCGTTGAGCCAAGTGGCACC
TACGAATATTCGACGTTTCAGACCGACAACAAACTGACCGTCAGCATCCGCCCGATGACACTCGACGACCTGCAAAAGCG
CAATGCCGATCGGCAGGCTTACGTCGGCGAAAAGCTTTCGCTGAATTTTCAGGACATCGATGTGCGCTCGGTGCTGCAAC
TGATCGCCGACTTCACCAATCTCAATCTGGTAGCCAGCGATACCGTGCAGGGCGGCATCACCTTGCGCCTGCAGAACGTG
CCGTGGGATCAGGCGCTGGATCTGGTGTTGAAAACCAAGGGGCTGGATAAACGCAAGGTCGGCAATGTCCTGTTGGTCGC
GCCGGCCGACGAGATCGCCGCCCGCGAGCGTCAGGAACTGGAATCGCAGAAGCAGATCGCCGAACTGGCTCCGCTGCGTC
GTGAGCTGTTGCAAGTGAACTACGCCAAGGCGGCAGACATCGCCAAGCTGTTCCAGTCTGTGACCAGTGCCGAGGCGAAA
GTCGATGAGCGCGGCTCGATCACTGTCGACGAGCGGACCAACAACATCATTGCTTACCAGACTCAGGATCGTCTCGACGA
ACTGCGGCGGATCGTGGCGCAACTGGATATTCCGGTACGCCAGGTGATGATCGAAGCGCGCATCGTCGAAGCCAACGTCG
ATTACGACAAGAGTCTGGGCGTGCGCTGGGGTGGTTCGGTGCAGAACAAGGGCAACTGGAACACCTCGGGGGTGAGCAAC
GGTTCATCGACCACCATTGGCACGCCGGGCAGCACCAGTACCAACTCGCCGTTCGTCGACATGGGCACCGTCAACAACAC
CTCCGGCATCGGGATCGCCTTCATTACCGACAATGTGTTGCTCGACCTTGAGCTGACCGCCATGGAAAAAACCGGCAACG
GCGAAATTGTCTCGCAGCCGAAAGTGGTCACCTCGGACAAAGAGACCGCAAAAATCCTCAAGGGCACGGAAATTCCTTAT
CAGGAGGCCAGTTCCAGCGGTGCGACGTCGGTGTCGTTCAAGGAGGCATCGCTGTCGCTGGAAGTGACACCGCAGATAAC
CCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAAGACGAACCGGATTACCTGAACAAAGTGCAGGATGTGCCGC
CGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGGTCAATGACGGCGAAACCATCGTGATTGGCGGTGTTTTCTCAAAT
ACTCAAAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTGTTTCTCACTCCGCGTATCATGAACAATCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(302-348)

Bacterial type II and III secretion system protein (Secretin)

(527-683)

AMIN domain (AMIN)

(37-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A448DKD5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.765

100

0.753
  pilQ Acinetobacter baumannii strain A118

38.267

100

0.415
  pilQ Acinetobacter baumannii D1279779

38.267

100

0.415
  comQ Acinetobacter baylyi ADP1

41.126

100

0.412
  pilQ Legionella pneumophila strain Lp02

37.814

97.832

0.37
  pilQ Legionella pneumophila strain ERS1305867

37.778

97.543

0.368

Multiple sequence alignment