Detailed information NTG1121313

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: EL257_RS01905
• Genome accession: NZ_LR134318
• Coordinates: 420343..421407 (+)
• Length: 354 a.a.
• NCBI ID: WP_126359321.1
• Uniprot ID: A0A448DKG5
• Organism: Pseudomonas fluorescens strain NCTC9428
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 415343..426407

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL257_RS01895 (NCTC9428_00384)	-	416268..417536 (+)	1269	WP_126359317.1	malic enzyme-like NAD(P)-binding protein	-
EL257_RS01900 (NCTC9428_00385)	-	417679..420123 (-)	2445	WP_419866605.1	penicillin-binding protein 1A	-
EL257_RS01905 (NCTC9428_00386)	pilM	420343..421407 (+)	1065	WP_126359321.1	pilus assembly protein PilM	Machinery gene
EL257_RS01910 (NCTC9428_00387)	-	421407..421973 (+)	567	WP_126359323.1	PilN domain-containing protein	-
EL257_RS01915 (NCTC9428_00388)	pilO	421970..422593 (+)	624	WP_126359325.1	type 4a pilus biogenesis protein PilO	-
EL257_RS01920 (NCTC9428_00389)	-	422590..423117 (+)	528	WP_126359326.1	pilus assembly protein PilP	-
EL257_RS01925 (NCTC9428_00390)	pilQ	423131..425209 (+)	2079	WP_126359328.1	type IV pilus secretin PilQ	Machinery gene
EL257_RS01930 (NCTC9428_00391)	aroK	425214..425732 (+)	519	WP_045122260.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38189.83 Da
• Isoelectric Point: 4.8983

>NTDB_id=1121313 EL257_RS01905 WP_126359321.1 420343..421407(+) (pilM) [Pseudomonas fluorescens strain NCTC9428]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPINAVVEKNIAELEGVGLALSRVLVKARTGSKGVAV
AVAGSAVITKIIEMDAWLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSARNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFSLLATQLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYVAEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLHTPTQVANP
FSNMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1121313 EL257_RS01905 WP_126359321.1 420343..421407(+) (pilM) [Pseudomonas fluorescens strain NCTC9428]
GTGCTGGGACTCTTCAATAAAAAGGCCAATACATTGCTGGGGATCGACATCAGCTCCACCTCGGTGAAGCTGTTGGAATT
GAGCCGTCAGGGTGATCGCTACCGGGTCGAGGCGTATGCGGTAGAGCCGCTGCCGATCAATGCGGTGGTCGAAAAGAACA
TCGCCGAACTCGAAGGCGTCGGCCTCGCCCTCAGTCGGGTGCTGGTCAAGGCGCGCACCGGCTCGAAAGGCGTGGCGGTG
GCCGTCGCCGGTTCGGCGGTGATCACCAAGATCATCGAAATGGACGCCTGGCTTTCCGACGACGAACTGGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATTCCCTATCCACTGGATGAAGTCGCCATCGACTTCGAAGTCCAGGGCGTGTCCGCGC
GCAATCCGGAGCGGGTCAACGTCTTGCTGGCCGCCTGCCGCAAGGAAAACGTCGAAGTGCGCGAAGCGGCATTGGCGCTG
GCCGGGCTGACAGCACGAGTGGTCGACGTAGAGGCCTACGCGCTGGAGCGCGCATTCAGTCTGCTGGCAACACAATTGGC
CGCTTCGCAAGAGCGTCTGACCGTCGCAGTGGTCGACATCGGCGCGACCATGACCACCCTCAGTGTCCTGCACAACGGCA
AAATCATTTATACGCGCGAGCAGCTGTTCGGCGGCCGGCAATTGACCGAGGAAATCCAGCGTCGCTACGGCCTGACCCTC
GAGCAAGCGGGACTTGCGAAAAAACAGGGAGGGTTGCCCGATGATTATGTCGCCGAGGTCTTGCAGCCGTTCCGCGAAGC
GCTGGTGCAGCAGGTTTCGCGCTCACTGCAGTTCTTCTTCGCCTCCGGGCAGTACAACGCCGTCGATCACATCCTGCTTG
CCGGTGGCACGGCCTCGGTGCCGGGGCTGGATCGGTTGATCGAGCAGCGCTTGCACACGCCGACCCAAGTGGCCAATCCG
TTTTCCAACATGGCCCTGAGCAGCAAAGTCAATGCGGGGGCGCTAGCCAGTGACGCGCCGGCGCTGATGATCGCTTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A448DKG5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	57.345	100	0.573
comM	Acinetobacter nosocomialis M2	57.345	100	0.573
comM	Acinetobacter baylyi ADP1	55.65	100	0.557
pilM	Legionella pneumophila strain ERS1305867	47.175	100	0.472