Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EL295_RS00295 Genome accession   NZ_LR134308
Coordinates   50240..51940 (-) Length   566 a.a.
NCBI ID   WP_126452973.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NCTC11445     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 45240..56940
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL295_RS00255 (NCTC11445_00048) - 45411..45836 (+) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  EL295_RS00260 (NCTC11445_00049) - 45833..46303 (+) 471 WP_003103866.1 hypothetical protein -
  EL295_RS00265 (NCTC11445_00050) - 46300..46989 (+) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  EL295_RS00270 (NCTC11445_00051) - 47095..47304 (+) 210 WP_003094660.1 hypothetical protein -
  EL295_RS00275 (NCTC11445_00052) yacG 47341..47541 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  EL295_RS00280 (NCTC11445_00053) coaE 47538..48149 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  EL295_RS00285 (NCTC11445_00054) pilD 48146..49018 (-) 873 WP_016264267.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  EL295_RS00290 (NCTC11445_00055) pilC 49019..50236 (-) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  EL295_RS00295 (NCTC11445_00056) pilB 50240..51940 (-) 1701 WP_126452973.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL295_RS00300 (NCTC11445_00057) - 52171..52635 (+) 465 WP_003107295.1 pilin -
  EL295_RS00305 (NCTC11445_00058) - 52727..54085 (+) 1359 WP_228778003.1 O-antigen ligase family protein -
  EL295_RS00315 (NCTC11445_00060) nadC 54266..55114 (-) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62312.29 Da        Isoelectric Point: 5.4470

>NTDB_id=1120928 EL295_RS00295 WP_126452973.1 50240..51940(-) (pilB) [Pseudomonas aeruginosa strain NCTC11445]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
VISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQDDGGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALLRIIMEEGNSIEIAEQVRKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1120928 EL295_RS00295 WP_126452973.1 50240..51940(-) (pilB) [Pseudomonas aeruginosa strain NCTC11445]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGT
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GTCATCAGCGAGCGCCTGGTCCGCCAGCACCGTGTGTTGCCGCTGTGGAAACGCGGCAACAAACTGTTCATCGGTCTCTC
CGACCCGACCAACCACCAGGCGGTCACCGATATCCAGTTCAGCACCGGTCTCACCACCGAAGCCCTGCTGGTGGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTCTACGAGGGCGCTATGGACGCTCTCGGCGACCTCGGTGACATCGATCTCGAC
GGGGTCGATGTCGGCAAGGCGGAAAGCGCCAGCCAGGACGACGGCGGCGACGATGCCGAAGATGCCCCGGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAAAAGACCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGCCGCATTTCCGCGCGACTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGACCAA
GTCGATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCACTGGGCTACGAAGAGGACCAGAAGGAACTCTACCTGGCCGCGCTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGTCTGAACATCCTCAATACCACCGA
CATCAACATCTCCACCGCTGAAGACCCTGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGTCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAGACTGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAAGGTTTCCCGGAA
GAGCTGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCTGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
TCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.544

100

0.58
  pilB Acinetobacter baylyi ADP1

56.614

100

0.567
  pilB Legionella pneumophila strain ERS1305867

54.321

100

0.544
  pilB Vibrio cholerae strain A1552

51.342

98.763

0.507
  pilF Neisseria gonorrhoeae MS11

49.823

99.647

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

51.97

94.17

0.489
  pilB Vibrio campbellii strain DS40M4

51.032

94.17

0.481
  pilB/pilB1 Synechocystis sp. PCC 6803

37.318

100

0.408
  pilF Thermus thermophilus HB27

38.489

100

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.545

97.173

0.375

Multiple sequence alignment