Detailed information NTG1120926

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EL295_RS00285
• Genome accession: NZ_LR134308
• Coordinates: 48146..49018 (-)
• Length: 290 a.a.
• NCBI ID: WP_016264267.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain NCTC11445
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 43146..54018

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL295_RS00240 (NCTC11445_00045)	-	44007..44177 (-)	171	WP_003094672.1	DUF3094 family protein	-
EL295_RS00245 (NCTC11445_00046)	-	44203..44652 (-)	450	WP_003094670.1	MOSC domain-containing protein	-
EL295_RS00250 (NCTC11445_00047)	-	44649..45278 (-)	630	WP_126452970.1	DUF1780 domain-containing protein	-
EL295_RS00255 (NCTC11445_00048)	-	45411..45836 (+)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
EL295_RS00260 (NCTC11445_00049)	-	45833..46303 (+)	471	WP_003103866.1	hypothetical protein	-
EL295_RS00265 (NCTC11445_00050)	-	46300..46989 (+)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
EL295_RS00270 (NCTC11445_00051)	-	47095..47304 (+)	210	WP_003094660.1	hypothetical protein	-
EL295_RS00275 (NCTC11445_00052)	yacG	47341..47541 (-)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
EL295_RS00280 (NCTC11445_00053)	coaE	47538..48149 (-)	612	WP_003094654.1	dephospho-CoA kinase	-
EL295_RS00285 (NCTC11445_00054)	pilD	48146..49018 (-)	873	WP_016264267.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
EL295_RS00290 (NCTC11445_00055)	pilC	49019..50236 (-)	1218	WP_003107299.1	type II secretion system F family protein	Machinery gene
EL295_RS00295 (NCTC11445_00056)	pilB	50240..51940 (-)	1701	WP_126452973.1	type IV-A pilus assembly ATPase PilB	Machinery gene
EL295_RS00300 (NCTC11445_00057)	-	52171..52635 (+)	465	WP_003107295.1	pilin	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31903.93 Da
• Isoelectric Point: 7.7068

>NTDB_id=1120926 EL295_RS00285 WP_016264267.1 48146..49018(-) (pilD) [Pseudomonas aeruginosa strain NCTC11445]
MTLLDYLASHPLAFVLCTILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGTVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=1120926 EL295_RS00285 WP_016264267.1 48146..49018(-) (pilD) [Pseudomonas aeruginosa strain NCTC11445]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCACCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCACGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55	96.552	0.531
pilD	Vibrio campbellii strain DS40M4	52.297	97.586	0.51
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Acinetobacter baumannii D1279779	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	48.561	95.862	0.466