Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EL104_RS10835 Genome accession   NZ_LR134298
Coordinates   2255713..2257098 (-) Length   461 a.a.
NCBI ID   WP_074865718.1    Uniprot ID   -
Organism   Pasteurella multocida subsp. gallicida strain NCTC10204     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2250713..2262098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL104_RS10805 (NCTC10204_02174) - 2250840..2252213 (-) 1374 WP_005722571.1 alanine/glycine:cation symporter family protein -
  EL104_RS10810 (NCTC10204_02176) - 2252662..2252931 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  EL104_RS10815 (NCTC10204_02177) yacG 2252931..2253134 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  EL104_RS10820 (NCTC10204_02178) coaE 2253145..2253765 (-) 621 WP_074865729.1 dephospho-CoA kinase -
  EL104_RS10825 (NCTC10204_02179) - 2253780..2254502 (-) 723 WP_074865725.1 prepilin peptidase -
  EL104_RS10830 (NCTC10204_02180) - 2254502..2255713 (-) 1212 WP_074865722.1 type II secretion system F family protein -
  EL104_RS10835 (NCTC10204_02181) pilB 2255713..2257098 (-) 1386 WP_074865718.1 GspE/PulE family protein Machinery gene
  EL104_RS10840 (NCTC10204_02182) pilA 2257098..2257532 (-) 435 WP_061406215.1 prepilin peptidase-dependent pilin Machinery gene
  EL104_RS10845 (NCTC10204_02183) ampD 2257692..2258258 (+) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EL104_RS10850 (NCTC10204_02184) rppH 2258844..2259311 (+) 468 Protein_2093 RNA pyrophosphohydrolase -
  EL104_RS10855 (NCTC10204_02185) - 2259443..2260237 (+) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  EL104_RS10860 (NCTC10204_02186) lgt 2260247..2261050 (+) 804 WP_074865713.1 prolipoprotein diacylglyceryl transferase -
  EL104_RS10865 (NCTC10204_02187) - 2261056..2261907 (+) 852 WP_005751072.1 thymidylate synthase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52110.70 Da        Isoelectric Point: 6.3696

>NTDB_id=1120698 EL104_RS10835 WP_074865718.1 2255713..2257098(-) (pilB) [Pasteurella multocida subsp. gallicida strain NCTC10204]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRAFQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCDCSV
ETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=1120698 EL104_RS10835 WP_074865718.1 2255713..2257098(-) (pilB) [Pasteurella multocida subsp. gallicida strain NCTC10204]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGTGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTTAAACGGGCATTTCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAACATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTTCAGCCTTCCTTTTCAGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACACATTTAGGCGAAAAAGCGGTATTGCGTTTACAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATG
ACCGCAGACCAACAACACAGATTCCAACACGCGTTAAGTCAGCCACAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTAAATACACAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACTGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCTCCCTCTGCTCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTATTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTGATTGCAGCGTA
GAGACACAATCGGCGTGTCGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-394)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

67.991

98.265

0.668
  pilB Haemophilus influenzae Rd KW20

67.329

98.265

0.662
  pilB Glaesserella parasuis strain SC1401

57.86

99.349

0.575
  pilB Acinetobacter baumannii D1279779

39.757

100

0.425
  pilB Vibrio campbellii strain DS40M4

39.419

100

0.412
  pilB Vibrio parahaemolyticus RIMD 2210633

39.293

100

0.41
  pilB Acinetobacter baylyi ADP1

37.675

100

0.408
  pilB Vibrio cholerae strain A1552

38.272

100

0.403
  pilB Legionella pneumophila strain ERS1305867

37.219

100

0.395
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.876

92.625

0.36

Multiple sequence alignment