Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EL104_RS10840 Genome accession   NZ_LR134298
Coordinates   2257098..2257532 (-) Length   144 a.a.
NCBI ID   WP_061406215.1    Uniprot ID   Q9LA12
Organism   Pasteurella multocida subsp. gallicida strain NCTC10204     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2252098..2262532
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL104_RS10810 (NCTC10204_02176) - 2252662..2252931 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  EL104_RS10815 (NCTC10204_02177) yacG 2252931..2253134 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  EL104_RS10820 (NCTC10204_02178) coaE 2253145..2253765 (-) 621 WP_074865729.1 dephospho-CoA kinase -
  EL104_RS10825 (NCTC10204_02179) - 2253780..2254502 (-) 723 WP_074865725.1 prepilin peptidase -
  EL104_RS10830 (NCTC10204_02180) - 2254502..2255713 (-) 1212 WP_074865722.1 type II secretion system F family protein -
  EL104_RS10835 (NCTC10204_02181) pilB 2255713..2257098 (-) 1386 WP_074865718.1 GspE/PulE family protein Machinery gene
  EL104_RS10840 (NCTC10204_02182) pilA 2257098..2257532 (-) 435 WP_061406215.1 prepilin peptidase-dependent pilin Machinery gene
  EL104_RS10845 (NCTC10204_02183) ampD 2257692..2258258 (+) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EL104_RS10850 (NCTC10204_02184) rppH 2258844..2259311 (+) 468 Protein_2093 RNA pyrophosphohydrolase -
  EL104_RS10855 (NCTC10204_02185) - 2259443..2260237 (+) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  EL104_RS10860 (NCTC10204_02186) lgt 2260247..2261050 (+) 804 WP_074865713.1 prolipoprotein diacylglyceryl transferase -
  EL104_RS10865 (NCTC10204_02187) - 2261056..2261907 (+) 852 WP_005751072.1 thymidylate synthase -
  EL104_RS10870 (NCTC10204_02188) tadA 2261922..2262422 (+) 501 WP_074865711.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15213.68 Da        Isoelectric Point: 9.1215

>NTDB_id=1120699 EL104_RS10840 WP_061406215.1 2257098..2257532(-) (pilA) [Pasteurella multocida subsp. gallicida strain NCTC10204]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNTPSECSAGQHGIR
ANISSGKKYLSSINVKSGVITVIGQKSLEGVNYTLSATGNAVDGVSWSVKCNGGSEVFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1120699 EL104_RS10840 WP_061406215.1 2257098..2257532(-) (pilA) [Pasteurella multocida subsp. gallicida strain NCTC10204]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCTGAGGTAGAGCTTTGTATCTATAATACAAATACGCCCAGTGAATGTAGTGCAGGACAGCATGGTATTAGA
GCAAATATAAGTTCTGGTAAAAAATATCTCAGCTCAATCAATGTCAAGTCTGGCGTGATTACTGTCATTGGTCAAAAAAG
CTTAGAGGGAGTCAATTACACTTTAAGCGCGACAGGAAATGCCGTTGATGGTGTTTCATGGAGTGTAAAATGTAATGGTG
GTAGTGAAGTTTTCCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9LA12

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

59.028

100

0.59
  pilA Haemophilus influenzae Rd KW20

59.028

100

0.59
  pilA Glaesserella parasuis strain SC1401

53.02

100

0.549
  comP Acinetobacter baylyi ADP1

37.681

95.833

0.361

Multiple sequence alignment