Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   EL087_RS04860 Genome accession   NZ_LR134288
Coordinates   987081..987755 (-) Length   224 a.a.
NCBI ID   WP_003024352.1    Uniprot ID   A0AAI9NIE3
Organism   Streptococcus milleri strain NCTC11169     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 982081..992755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL087_RS04845 (NCTC11169_00965) - 982161..983774 (-) 1614 WP_126407165.1 M20/M25/M40 family metallo-hydrolase -
  EL087_RS04850 (NCTC11169_00966) - 983951..985399 (+) 1449 WP_126407167.1 alpha-amylase -
  EL087_RS04855 (NCTC11169_00967) ciaH 985745..987091 (-) 1347 WP_106083386.1 sensor histidine kinase Regulator
  EL087_RS04860 (NCTC11169_00968) ciaR 987081..987755 (-) 675 WP_003024352.1 response regulator transcription factor Regulator
  EL087_RS04865 (NCTC11169_00969) - 988028..988480 (-) 453 WP_126407169.1 hypothetical protein -
  EL087_RS09350 (NCTC11169_00970) - 988956..989357 (-) 402 WP_232008231.1 hypothetical protein -
  EL087_RS04880 (NCTC11169_00973) rplS 989800..990147 (-) 348 WP_003024326.1 50S ribosomal protein L19 -
  EL087_RS04885 (NCTC11169_00974) - 990297..991256 (-) 960 WP_126407171.1 ROK family glucokinase -
  EL087_RS09355 (NCTC11169_00975) - 991477..991980 (+) 504 WP_232008232.1 LysR family transcriptional regulator -
  EL087_RS09360 (NCTC11169_00976) - 992036..992320 (+) 285 WP_232008233.1 hypothetical protein -

Sequence

Protein


Download         Length: 224 a.a.        Molecular weight: 25359.01 Da        Isoelectric Point: 4.2408

>NTDB_id=1120266 EL087_RS04860 WP_003024352.1 987081..987755(-) (ciaR) [Streptococcus milleri strain NCTC11169]
MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGVYDLILLDLMLPEKDGFQVLKELREKGVTTPVLIMTA
KESLNDKGHGFELGADDYLTKPFYLEELKMRIQALLKRSGKFNENTLSYGDVTVNLSTNTTLVDGNEVELLGKEFDLLVY
FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKIRKKLKGTTFANNLQTLRSVGYILKDAE

Nucleotide


Download         Length: 675 bp        

>NTDB_id=1120266 EL087_RS04860 WP_003024352.1 987081..987755(-) (ciaR) [Streptococcus milleri strain NCTC11169]
ATGATTAAGATTCTATTAGTGGAAGACGACCTCGGTTTATCTAATTCAGTATTTGACTTTTTGGATGATTTTGCAGATGT
CATGCAAGTTTTTGATGGTGAGGAAGGATTGTATGAGGCTGAGAGTGGCGTGTACGACCTTATCTTGCTTGACTTGATGT
TGCCAGAAAAAGACGGCTTCCAAGTGTTGAAAGAATTGCGTGAAAAAGGCGTGACAACACCTGTTTTGATTATGACAGCC
AAAGAAAGTTTGAATGATAAAGGACATGGTTTTGAACTGGGTGCTGATGATTATTTGACCAAGCCATTTTATCTAGAAGA
GTTAAAAATGCGGATTCAAGCTCTGTTGAAGCGTTCTGGTAAGTTTAATGAAAACACCCTTTCTTATGGCGATGTCACTG
TTAATTTATCAACTAACACAACACTTGTAGACGGAAATGAAGTAGAATTGCTCGGAAAAGAATTTGACTTGCTGGTGTAT
TTCTTGCAGAATCAAAATGTCATTTTGCCTAAGACACAGATTTTCGATCGCTTGTGGGGATTTGATAGTGATACAACGAT
TTCAGTCGTAGAAGTCTATGTTTCAAAAATCCGGAAAAAATTGAAAGGGACGACCTTTGCGAACAATCTTCAAACGCTTC
GCAGCGTCGGATATATTTTAAAAGATGCTGAATAA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(4-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

91.964

100

0.92
  ciaR Streptococcus pneumoniae D39

91.964

100

0.92
  ciaR Streptococcus pneumoniae R6

91.964

100

0.92
  ciaR Streptococcus pneumoniae TIGR4

91.964

100

0.92
  ciaR Streptococcus mutans UA159

90.135

99.554

0.897
  covR Lactococcus lactis subsp. lactis strain DGCC12653

38.767

100

0.393
  vicR Streptococcus mutans UA159

36.052

100

0.375
  covR Streptococcus salivarius strain HSISS4

35.683

100

0.362

Multiple sequence alignment