Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EL111_RS02135 Genome accession   NZ_LR134287
Coordinates   445181..446860 (-) Length   559 a.a.
NCBI ID   WP_123795647.1    Uniprot ID   A0A5P3MRH5
Organism   Neisseria animalis strain NCTC10212     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 440181..451860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL111_RS02110 (NCTC10212_00426) ispH 440578..441546 (+) 969 WP_123795651.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  EL111_RS10465 - 441645..441818 (-) 174 WP_162842984.1 hypothetical protein -
  EL111_RS02115 (NCTC10212_00427) yacG 442173..442361 (-) 189 WP_197717779.1 DNA gyrase inhibitor YacG -
  EL111_RS02120 (NCTC10212_00428) coaE 442413..443033 (-) 621 WP_123795649.1 dephospho-CoA kinase -
  EL111_RS02125 (NCTC10212_00429) pilD 443066..443926 (-) 861 WP_123795648.1 prepilin peptidase Machinery gene
  EL111_RS02130 (NCTC10212_00430) pilG 443928..445172 (-) 1245 WP_269471079.1 type II secretion system F family protein Machinery gene
  EL111_RS02135 (NCTC10212_00431) pilF 445181..446860 (-) 1680 WP_123795647.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL111_RS02140 (NCTC10212_00432) - 447416..447922 (-) 507 WP_123795646.1 pilin -
  EL111_RS02145 (NCTC10212_00433) - 448677..450728 (-) 2052 WP_123795645.1 M3 family metallopeptidase -
  EL111_RS02150 (NCTC10212_00434) - 451005..451859 (-) 855 WP_123795644.1 mechanosensitive ion channel family protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62047.50 Da        Isoelectric Point: 6.3932

>NTDB_id=1120201 EL111_RS02135 WP_123795647.1 445181..446860(-) (pilF) [Neisseria animalis strain NCTC10212]
MSVGLLRVLSQHHVLTEERIGYYTAAAQSGRAVLPMLFADGIITPRALGEMLAATFNYPLLDLQHVPRTHILTDIITEEQ
MLKNRCVPLFRRGHTVYLAVSDPTRIQDFQKMAFAAGETMDLVIVRDDQLSALLEWLGQRSTSILQEIRHEQETVRGTQI
LYIDNEEAEDGPIPRFIHKTLSDAIQAGASDIHFEFYEHIARVRFRVDGQLREVVQPPVAVRGQLASRIKVMAHLDIAEK
RVPQDGRIQIAFEKNGRPIDFRVSTLPTLFGEKVVMRILNSDLGDLNIDTLGLEPFQKDMLLDAIGRPYGMVLVTGPTGS
GKTVSLYTCLNILNTEKVNIATAEDPAEINLPGINQVNVNDKQGLTFASALRAFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRLLNMGVAPFNIASSISLIVAQRLLRCLCPHCKKAVARPPNTVLKQAGFTDEDLQQPWTL
YRPAGCESCRGSGFKGRIGVYEVMPVSKEMQHVIMDNGTEVDITNLAYQEGIVDLRRAGLLKVMRGLTSLEEVTARTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=1120201 EL111_RS02135 WP_123795647.1 445181..446860(-) (pilF) [Neisseria animalis strain NCTC10212]
ATGAGCGTCGGATTATTGCGCGTGCTTTCCCAGCATCACGTCCTAACCGAAGAGCGTATCGGATACTACACAGCCGCCGC
ACAATCGGGCAGAGCGGTACTGCCCATGCTTTTTGCAGACGGCATCATCACACCGCGCGCGCTGGGAGAAATGCTTGCCG
CCACGTTCAATTACCCGCTGCTCGACTTGCAGCACGTTCCGCGCACCCATATCCTGACCGACATCATTACCGAAGAGCAA
ATGTTGAAAAACCGCTGCGTACCGCTGTTTCGGCGCGGACACACGGTTTATCTGGCGGTTTCCGATCCTACGCGCATTCA
GGATTTCCAAAAAATGGCGTTTGCCGCCGGCGAAACAATGGATTTGGTTATCGTGCGCGATGACCAGTTATCCGCCTTAT
TGGAATGGCTGGGGCAGCGTTCGACCAGCATTTTGCAAGAAATCCGCCACGAACAGGAAACGGTTCGCGGCACACAGATT
TTATACATCGACAACGAAGAGGCCGAAGACGGCCCGATTCCGCGCTTTATCCATAAAACCTTATCCGACGCCATTCAGGC
GGGCGCATCGGATATTCATTTTGAATTTTACGAACACATCGCCAGAGTACGTTTCCGCGTGGACGGCCAACTGCGCGAAG
TGGTGCAGCCGCCTGTTGCCGTGCGCGGCCAGCTTGCTTCACGAATCAAAGTCATGGCGCATTTGGATATTGCTGAAAAA
CGTGTCCCGCAAGACGGCCGTATCCAAATTGCTTTTGAAAAAAACGGCCGTCCGATAGACTTCCGTGTCAGCACTTTGCC
AACGCTGTTTGGCGAAAAAGTCGTGATGCGGATTTTAAATTCCGATTTGGGCGATTTGAATATCGATACCTTGGGCTTGG
AGCCGTTTCAAAAAGATATGCTGCTCGATGCCATCGGCCGTCCTTACGGCATGGTGCTGGTTACCGGCCCGACCGGTTCC
GGCAAAACCGTATCGCTTTATACCTGCCTGAACATTCTGAATACGGAGAAAGTCAATATCGCCACGGCTGAAGATCCGGC
AGAAATCAACCTGCCCGGCATCAATCAAGTCAATGTCAACGACAAACAAGGACTGACGTTCGCCTCCGCGCTGCGCGCTT
TCCTTCGCCAAGACCCCGACATCATCATGGTAGGGGAAATCCGCGATTTGGAAACTGCCGACATCGCCATCAAAGCCGCG
CAAACCGGCCACATGGTGTTTTCCACCCTCCATACCAACAATGCGCCCGCCACTTTGTCGAGGCTGCTGAATATGGGCGT
TGCCCCGTTCAACATCGCCAGCTCCATCAGCCTGATTGTGGCGCAACGCCTGCTGCGCTGCTTATGCCCGCACTGTAAAA
AAGCCGTTGCCCGTCCGCCTAATACCGTATTGAAGCAGGCCGGATTTACCGATGAAGACTTGCAGCAGCCGTGGACGCTG
TACCGCCCTGCCGGTTGCGAATCCTGCCGCGGCAGTGGTTTCAAAGGGCGTATCGGCGTATATGAAGTCATGCCGGTCAG
CAAGGAAATGCAGCATGTCATCATGGATAACGGCACGGAAGTGGACATCACCAATCTGGCTTATCAGGAAGGCATAGTAG
ACTTGCGCCGCGCCGGTTTGCTGAAAGTCATGAGGGGGCTGACTTCGCTGGAAGAAGTAACCGCCCGTACCAATGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-140)

Type II/IV secretion system protein (T2SSE)

(179-446)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P3MRH5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

72.987

100

0.73
  pilB Acinetobacter baylyi ADP1

51.02

96.422

0.492
  pilB Acinetobacter baumannii D1279779

48.925

99.821

0.488
  pilB Legionella pneumophila strain ERS1305867

47.905

98.211

0.47
  pilB Vibrio cholerae strain A1552

45.841

100

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

44.523

100

0.451
  pilB Vibrio campbellii strain DS40M4

44.071

100

0.445
  pilF Thermus thermophilus HB27

38.447

94.454

0.363
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.623

93.56

0.361

Multiple sequence alignment