Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   EL076_RS09115 Genome accession   NZ_LR134275
Coordinates   1803548..1803985 (-) Length   145 a.a.
NCBI ID   WP_003091908.1    Uniprot ID   A0A3S4LUG5
Organism   Streptococcus vestibularis strain NCTC12167     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1798548..1808985
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL076_RS09080 (NCTC12167_01791) proC 1798558..1799328 (+) 771 WP_064520010.1 pyrroline-5-carboxylate reductase -
  EL076_RS09085 (NCTC12167_01792) - 1799358..1800020 (-) 663 WP_003095899.1 CPBP family intramembrane glutamic endopeptidase -
  EL076_RS10515 - 1800120..1800590 (-) 471 WP_082901122.1 CAAX protease -
  EL076_RS09095 (NCTC12167_01794) - 1800577..1800774 (-) 198 WP_002885716.1 helix-turn-helix transcriptional regulator -
  EL076_RS09100 (NCTC12167_01795) - 1801002..1802195 (-) 1194 WP_064520007.1 acetate kinase -
  EL076_RS09105 (NCTC12167_01796) comYH 1802252..1803208 (-) 957 WP_126430075.1 class I SAM-dependent methyltransferase Machinery gene
  EL076_RS09110 (NCTC12167_01797) comGG 1803253..1803570 (-) 318 WP_003092009.1 competence type IV pilus minor pilin ComGG -
  EL076_RS09115 (NCTC12167_01798) comYF 1803548..1803985 (-) 438 WP_003091908.1 competence type IV pilus minor pilin ComGF Machinery gene
  EL076_RS09120 (NCTC12167_01799) comGE 1803972..1804202 (-) 231 WP_003095891.1 competence type IV pilus minor pilin ComGE -
  EL076_RS09125 (NCTC12167_01800) comYD 1804234..1804662 (-) 429 WP_253665936.1 competence type IV pilus minor pilin ComGD Machinery gene
  EL076_RS09130 (NCTC12167_01801) comYC 1804622..1804948 (-) 327 WP_002885815.1 competence type IV pilus major pilin ComGC Machinery gene
  EL076_RS09135 (NCTC12167_01802) comYB 1804945..1806045 (-) 1101 WP_126430077.1 competence type IV pilus assembly protein ComGB Machinery gene
  EL076_RS09140 (NCTC12167_01803) comGA/cglA/cilD 1805927..1806868 (-) 942 WP_126430079.1 competence type IV pilus ATPase ComGA Machinery gene
  EL076_RS09145 (NCTC12167_01804) tnpA 1807052..1807516 (-) 465 WP_002883780.1 IS200/IS605 family transposase -
  EL076_RS09150 (NCTC12167_01805) - 1807715..1808077 (-) 363 WP_126430081.1 DUF1033 family protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16211.64 Da        Isoelectric Point: 8.4658

>NTDB_id=1119915 EL076_RS09115 WP_003091908.1 1803548..1803985(-) (comYF) [Streptococcus vestibularis strain NCTC12167]
MSLNSKVRAFTLLECLVALLVIAGSVQVYQGLTTVLVGNVKQVRQQENQDWLLFVQQMEAELEGCQLVKVEGNKLYVKQD
NQDLAFGLSSATDFRKTNADGRGYQPMLFDVKSSTVSQKNQIVTIQVTLKNGLQRSFIYAFEKTG

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1119915 EL076_RS09115 WP_003091908.1 1803548..1803985(-) (comYF) [Streptococcus vestibularis strain NCTC12167]
ATGTCTCTAAATAGTAAGGTTCGCGCCTTTACCCTTTTGGAATGTCTGGTAGCCTTACTGGTTATAGCTGGTTCTGTCCA
AGTATATCAGGGCTTAACCACCGTTCTGGTGGGCAATGTCAAACAGGTAAGACAACAAGAAAACCAAGACTGGCTTCTAT
TTGTCCAACAAATGGAGGCAGAATTAGAAGGTTGTCAGTTGGTAAAGGTAGAGGGGAACAAACTCTATGTCAAGCAGGAT
AATCAAGACCTGGCTTTTGGACTTTCAAGTGCTACTGATTTTCGAAAAACAAATGCTGACGGTCGAGGCTATCAACCAAT
GCTTTTCGATGTCAAATCTAGTACGGTAAGTCAAAAGAACCAGATAGTTACTATTCAAGTGACTCTTAAAAATGGACTAC
AAAGGAGTTTTATTTATGCTTTTGAAAAAACAGGTTAG

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S4LUG5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

56.028

97.241

0.545
  comYF Streptococcus mutans UA159

55.319

97.241

0.538
  comGF/cglF Streptococcus pneumoniae R6

50

97.931

0.49
  comGF/cglF Streptococcus pneumoniae TIGR4

50

97.931

0.49
  comGF/cglF Streptococcus pneumoniae Rx1

50

97.931

0.49
  comGF/cglF Streptococcus pneumoniae D39

50

97.931

0.49
  comGF/cglF Streptococcus mitis NCTC 12261

47.222

99.31

0.469
  comGF/cglF Streptococcus mitis SK321

47.222

99.31

0.469
  comGF Lactococcus lactis subsp. cremoris KW2

46.043

95.862

0.441

Multiple sequence alignment