Detailed information NTG1119826

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: EL086_RS06170
• Genome accession: NZ_LR134274
• Coordinates: 1264843..1265517 (-)
• Length: 224 a.a.
• NCBI ID: WP_002891134.1
• Uniprot ID: J7TS49
• Organism: Streptococcus salivarius strain NCTC8618
• Function: repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 1259843..1270517

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL086_RS06145 (NCTC8618_03654)	-	1260157..1261434 (-)	1278	WP_014634600.1	pyrimidine-nucleoside phosphorylase	-
EL086_RS06150 (NCTC8618_03655)	-	1261431..1262021 (-)	591	WP_014633038.1	class I SAM-dependent methyltransferase	-
EL086_RS06155 (NCTC8618_03656)	coaA	1262134..1263054 (+)	921	WP_038676170.1	type I pantothenate kinase	-
EL086_RS06160 (NCTC8618_03657)	rpsT	1263124..1263360 (+)	237	WP_004182129.1	30S ribosomal protein S20	-
EL086_RS06165 (NCTC8618_03658)	ciaH	1263438..1264853 (-)	1416	WP_004182392.1	sensor histidine kinase	Regulator
EL086_RS06170 (NCTC8618_03659)	ciaR	1264843..1265517 (-)	675	WP_002891134.1	response regulator transcription factor	Regulator
EL086_RS06175 (NCTC8618_03660)	-	1265715..1267385 (-)	1671	WP_004182072.1	formate--tetrahydrofolate ligase	-
EL086_RS06180 (NCTC8618_03661)	-	1267580..1268263 (+)	684	WP_022496696.1	phosphopantothenate--cysteine ligase	-
EL086_RS06185 (NCTC8618_03662)	coaC	1268256..1268801 (+)	546	WP_022496697.1	phosphopantothenoylcysteine decarboxylase	-
EL086_RS06190 (NCTC8618_03663)	-	1268830..1269402 (+)	573	WP_022496698.1	ECF transporter S component	-

Sequence

Protein

• Length: 224 a.a.
• Molecular weight: 25362.02 Da
• Isoelectric Point: 4.2897

>NTDB_id=1119826 EL086_RS06170 WP_002891134.1 1264843..1265517(-) (ciaR) [Streptococcus salivarius strain NCTC8618]
MIKILLVEDDLSLSNSVFDFLDDFADVMQVFDGDEGLYEAESGVYDLILLDLMLPEKDGFTVLRELREKGVTTPVLIMTA
KESLDDKGHGFELGADDYLTKPFYLEELKMRIQALLKRSGKFDQNTLSFGDVRVNLATNSTFVGDKEVELLGKEFDLLVY
FLQNQNVILPKSQIFDRLWGFDSDTTVSVVEVYVSKIRKKLKGTDFAANLQTLRSVGYILKNAD

Nucleotide

• Length: 675 bp

>NTDB_id=1119826 EL086_RS06170 WP_002891134.1 1264843..1265517(-) (ciaR) [Streptococcus salivarius strain NCTC8618]
ATGATTAAAATACTACTAGTAGAAGATGATTTGAGTTTGTCGAACTCAGTTTTTGATTTTTTAGATGACTTTGCTGATGT
GATGCAAGTCTTTGATGGAGATGAAGGTCTCTACGAAGCTGAAAGTGGCGTCTATGATTTGATTCTTTTAGACCTTATGT
TACCTGAAAAAGATGGTTTCACGGTTCTTAGAGAATTACGTGAAAAAGGTGTAACAACTCCTGTACTTATCATGACGGCT
AAGGAAAGTCTAGATGATAAGGGTCATGGCTTTGAACTGGGTGCAGACGACTATCTAACCAAACCTTTCTATTTAGAAGA
GTTGAAGATGCGTATTCAAGCACTTTTGAAACGATCTGGTAAATTTGACCAAAACACACTTTCTTTTGGTGATGTTCGTG
TCAATCTTGCTACAAATTCAACCTTTGTGGGGGATAAAGAAGTAGAACTTCTTGGTAAAGAGTTTGATTTATTAGTTTAC
TTCTTACAAAATCAAAACGTAATTTTGCCTAAGTCACAAATTTTTGACCGTCTTTGGGGATTTGATAGTGATACCACAGT
ATCAGTTGTTGAGGTTTATGTTTCTAAAATTCGTAAGAAACTTAAAGGAACAGACTTTGCTGCCAATCTCCAAACCTTGA
GAAGTGTGGGGTATATTTTGAAGAATGCTGACTAA

Domains

Predicted by InterproScan.

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (146-220)

• Response regulator receiver domain (Response_reg) (4-112)

3D structure

• AlphaFold DB: J7TS49

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus pneumoniae Rx1	87.444	99.554	0.871
ciaR	Streptococcus pneumoniae D39	87.444	99.554	0.871
ciaR	Streptococcus pneumoniae R6	87.444	99.554	0.871
ciaR	Streptococcus pneumoniae TIGR4	87.444	99.554	0.871
ciaR	Streptococcus mutans UA159	86.607	100	0.866
covR	Lactococcus lactis subsp. lactis strain DGCC12653	38.222	100	0.384
vicR	Streptococcus mutans UA159	35.983	100	0.384
micA	Streptococcus pneumoniae Cp1015	34.322	100	0.362