Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EL022_RS08040 Genome accession   NZ_LR134173
Coordinates   1869583..1871688 (+) Length   701 a.a.
NCBI ID   WP_028382181.1    Uniprot ID   -
Organism   Legionella cherrii strain NCTC11976     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1864583..1876688
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL022_RS08015 (NCTC11976_01669) - 1865471..1866550 (-) 1080 WP_051544504.1 hypothetical protein -
  EL022_RS08020 (NCTC11976_01670) pilM 1866793..1867857 (+) 1065 WP_028382185.1 pilus assembly protein PilM Machinery gene
  EL022_RS08025 (NCTC11976_01671) pilN 1867864..1868451 (+) 588 WP_028382184.1 PilN domain-containing protein Machinery gene
  EL022_RS08030 (NCTC11976_01672) pilO 1868448..1869047 (+) 600 WP_028382183.1 type 4a pilus biogenesis protein PilO Machinery gene
  EL022_RS08035 (NCTC11976_01673) pilP 1869044..1869574 (+) 531 WP_028382182.1 pilus assembly protein PilP Machinery gene
  EL022_RS08040 (NCTC11976_01674) pilQ 1869583..1871688 (+) 2106 WP_028382181.1 type IV pilus secretin PilQ Machinery gene
  EL022_RS08045 (NCTC11976_01675) aroK 1872382..1872903 (+) 522 WP_028382180.1 shikimate kinase AroK -
  EL022_RS08050 (NCTC11976_01676) aroB 1872890..1873993 (+) 1104 WP_028382179.1 3-dehydroquinate synthase -
  EL022_RS08055 (NCTC11976_01677) - 1873996..1875435 (+) 1440 WP_028382178.1 SPOR domain-containing protein -
  EL022_RS08060 (NCTC11976_01678) proC 1875675..1876511 (-) 837 WP_028382177.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 77668.02 Da        Isoelectric Point: 9.7319

>NTDB_id=1118769 EL022_RS08040 WP_028382181.1 1869583..1871688(+) (pilQ) [Legionella cherrii strain NCTC11976]
MRRITALFIFIGMSLCMMAFAQNNPLESVKVVPLPEEKLRLDFQFAKPLKQEPASFVTQKPARIVLDFIDSNLQLPPEQR
TKEIKLGSLNTYTIVAVGSRVRAILDLNFPVPYSGTISGKVYTLILNGKSNELFQPPKEILITNKPVKTSYEINHFDFRG
IERQGGRAIVNVSNSSVPIEVEEHGKEILVKFLNTNIPQNLRKRFDVSDFRSPVQLVAFQQAGKNAHMILSNKGNFGQYV
YQINKQFMIDVFPLTQEEAQQAKLKKKVFSGRRISLNFQNISIRAVLQLLADFTGINIVVSDKVQGNITLRLNNIPWDQA
LDIILTTQGLAKRQTGNVMLIDYKASFDKMEAEELKSQHIIQKLEPVRSELIQINYAKATDLAILIKDKQNSLLSEKGKI
SVDTRTNTIWIQDSGTKIDEVRDLIKQLDVPVKQVLIEARIVEVTKDFAQDLGIRWGVSKPPHLSGTLAGANQLEQGVAP
ANVVPFTDRLNLDLVAAPVTGAVPASVGIALAQLGDNILLDLELSALESEGLAELISSPRLITANQQPAVIDSGQEIPYQ
EATSSGATAVAFKKAVLSLKVTPQITPDSKILMELKINQDTASPQLFNGVPAILTKEIQTNVLVSNGQTIVLGGIYQQDK
SKTITRVPFFGQLPVVGNLFKNTQIALKNDELLIFITPKIITNSLSITTIEGRKPIRFDEK

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=1118769 EL022_RS08040 WP_028382181.1 1869583..1871688(+) (pilQ) [Legionella cherrii strain NCTC11976]
GTGCGGAGAATAACTGCTTTATTCATTTTTATTGGCATGAGCTTATGTATGATGGCTTTTGCCCAAAATAATCCTCTTGA
ATCGGTAAAGGTAGTTCCTTTGCCTGAGGAAAAGTTGCGTTTGGATTTCCAATTTGCGAAACCTCTTAAGCAAGAACCTG
CAAGTTTTGTGACGCAAAAACCGGCACGTATCGTTCTAGATTTCATTGATTCCAACCTGCAATTACCTCCAGAGCAGAGG
ACGAAAGAAATTAAATTAGGTTCACTAAATACCTATACTATTGTGGCCGTGGGGTCGCGTGTTCGAGCTATATTGGATTT
AAATTTCCCTGTACCTTATTCCGGTACAATTTCTGGAAAAGTTTATACACTGATTTTGAATGGCAAAAGCAACGAGTTAT
TTCAACCTCCTAAAGAAATTTTAATCACCAATAAACCGGTCAAGACAAGTTATGAAATCAATCATTTTGACTTTCGTGGG
ATTGAGAGACAAGGCGGCCGTGCAATAGTCAATGTTTCCAATAGCAGTGTTCCAATTGAGGTAGAGGAACATGGTAAAGA
AATTCTAGTAAAATTTTTAAATACAAATATTCCACAGAATCTAAGGAAACGTTTTGATGTTTCCGATTTCCGCAGCCCAG
TCCAACTAGTTGCTTTTCAGCAAGCCGGTAAAAACGCGCACATGATTTTGTCTAACAAGGGTAATTTTGGTCAATATGTT
TATCAAATTAATAAACAATTCATGATTGATGTCTTTCCCTTAACTCAAGAAGAAGCGCAACAGGCTAAATTGAAAAAGAA
GGTATTTTCTGGAAGAAGAATTTCTTTGAATTTTCAGAATATCAGCATACGCGCTGTTTTACAGTTATTGGCTGATTTTA
CCGGAATTAATATTGTCGTAAGCGATAAAGTTCAGGGAAATATCACTCTAAGATTAAATAACATTCCCTGGGATCAGGCT
TTAGATATTATTTTAACTACGCAAGGACTCGCTAAGCGTCAGACAGGTAATGTGATGTTGATCGATTATAAAGCTTCTTT
TGATAAAATGGAGGCAGAAGAGCTTAAAAGTCAGCATATTATTCAGAAACTTGAACCGGTGCGTTCCGAACTGATTCAGA
TTAATTATGCAAAAGCTACTGATCTTGCGATTTTAATTAAAGACAAACAAAATTCACTTCTATCTGAAAAGGGTAAAATA
AGTGTTGATACACGAACAAATACCATTTGGATTCAAGACAGCGGAACCAAAATCGATGAAGTCAGGGATTTGATAAAACA
GTTGGATGTACCGGTTAAACAAGTACTTATTGAGGCACGTATTGTCGAAGTCACTAAAGATTTTGCGCAAGACTTGGGAA
TACGTTGGGGTGTTTCCAAACCACCTCATTTAAGTGGTACTTTAGCTGGGGCGAACCAACTGGAGCAGGGGGTTGCTCCT
GCAAATGTCGTACCGTTTACAGACCGATTAAACTTGGATTTGGTTGCTGCTCCTGTGACAGGAGCAGTCCCTGCCTCCGT
TGGTATTGCATTGGCTCAGCTTGGCGATAATATTTTACTTGATCTTGAGTTATCAGCTTTGGAAAGTGAAGGGCTTGCTG
AATTGATATCCAGTCCGAGACTTATTACTGCGAATCAACAGCCTGCAGTAATTGATTCAGGACAGGAGATACCTTATCAG
GAAGCCACTTCAAGTGGTGCGACTGCTGTAGCGTTCAAAAAAGCGGTATTAAGTTTAAAGGTTACTCCGCAAATTACTCC
TGATAGTAAAATACTTATGGAGTTAAAAATTAATCAGGATACGGCCTCACCCCAATTATTTAACGGTGTGCCTGCTATTT
TGACTAAGGAAATTCAGACTAATGTCCTTGTTAGTAATGGACAGACTATAGTTCTAGGGGGAATCTATCAGCAAGACAAG
AGTAAAACGATAACCAGAGTACCTTTTTTTGGTCAGTTGCCTGTAGTCGGAAATTTATTTAAAAATACCCAAATTGCTCT
TAAAAACGATGAATTACTTATTTTTATTACACCAAAAATAATAACCAATTCATTATCGATTACGACAATTGAAGGCAGAA
AACCGATTCGTTTTGATGAAAAATAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(370-434)

Secretin and TonB N terminus short domain (STN)

(296-341)

Bacterial type II and III secretion system protein (Secretin)

(526-681)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Legionella pneumophila strain Lp02

74.889

96.576

0.723
  pilQ Legionella pneumophila strain ERS1305867

74.815

96.291

0.72
  pilQ Pseudomonas aeruginosa PAK

36.3

100

0.372
  comQ Acinetobacter baylyi ADP1

37.069

99.287

0.368

Multiple sequence alignment