Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   EL022_RS08020 Genome accession   NZ_LR134173
Coordinates   1866793..1867857 (+) Length   354 a.a.
NCBI ID   WP_028382185.1    Uniprot ID   -
Organism   Legionella cherrii strain NCTC11976     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1861793..1872857
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL022_RS08010 (NCTC11976_01668) - 1862913..1865279 (-) 2367 WP_028382186.1 penicillin-binding protein 1A -
  EL022_RS08015 (NCTC11976_01669) - 1865471..1866550 (-) 1080 WP_051544504.1 hypothetical protein -
  EL022_RS08020 (NCTC11976_01670) pilM 1866793..1867857 (+) 1065 WP_028382185.1 pilus assembly protein PilM Machinery gene
  EL022_RS08025 (NCTC11976_01671) pilN 1867864..1868451 (+) 588 WP_028382184.1 PilN domain-containing protein Machinery gene
  EL022_RS08030 (NCTC11976_01672) pilO 1868448..1869047 (+) 600 WP_028382183.1 type 4a pilus biogenesis protein PilO Machinery gene
  EL022_RS08035 (NCTC11976_01673) pilP 1869044..1869574 (+) 531 WP_028382182.1 pilus assembly protein PilP Machinery gene
  EL022_RS08040 (NCTC11976_01674) pilQ 1869583..1871688 (+) 2106 WP_028382181.1 type IV pilus secretin PilQ Machinery gene

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39084.94 Da        Isoelectric Point: 4.5544

>NTDB_id=1118765 EL022_RS08020 WP_028382185.1 1866793..1867857(+) (pilM) [Legionella cherrii strain NCTC11976]
MLKLFQPKHRSILGIDITSTSVKVLEISGQEGALVVENYGREVLPSNAMDGNTIKDIDVVSRCIKKVIDRLHTSCKQVAL
AVPDAAIISKVIQINEGLNDEEMEELIVTEADKYIPYPIDEINLDFEILGQSEKNPHMLDVLIVASRAENVNQRVETAVR
AGLDAIVIDVESYAVERAAQQIAKDLPASGQDKTIAIIDVGASYTHLFVLQGMKLVYSREEKFGGKQLIESIAEHYKMTL
EQATLAKENGELPEDYEKEVLEPFKEDILLQIKRTLQFFYSTSQDGDVDHILLAGGLAKLPGLVSLVQERLGMSTTIANP
FSYMTPGKMMNLDAVNNDAPSLMVACGLALRNIR

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1118765 EL022_RS08020 WP_028382185.1 1866793..1867857(+) (pilM) [Legionella cherrii strain NCTC11976]
TTGCTCAAATTGTTTCAGCCCAAACATCGTTCAATTCTCGGAATAGACATTACATCAACGTCGGTTAAAGTATTGGAAAT
TTCAGGCCAAGAAGGTGCGCTTGTTGTGGAAAACTATGGACGTGAGGTATTACCATCAAACGCCATGGATGGGAACACAA
TTAAGGATATTGATGTGGTTTCCCGTTGTATTAAGAAAGTAATTGATCGCTTGCATACATCATGCAAGCAGGTTGCCTTA
GCCGTTCCTGATGCGGCAATAATTAGCAAGGTCATACAAATCAATGAGGGTTTAAATGATGAGGAAATGGAGGAACTGAT
TGTTACTGAGGCTGATAAATACATTCCCTATCCAATCGACGAAATCAATCTTGATTTTGAAATTTTAGGCCAGTCTGAAA
AAAATCCACATATGTTAGATGTTCTTATTGTTGCATCACGAGCGGAAAATGTGAATCAGCGTGTTGAGACTGCAGTTCGT
GCAGGCTTAGATGCAATAGTGATTGACGTAGAGTCTTATGCTGTCGAACGAGCGGCTCAACAGATTGCTAAAGATTTACC
TGCATCAGGACAAGATAAAACAATAGCGATCATTGATGTTGGTGCGAGTTATACCCACTTATTTGTATTACAGGGAATGA
AGTTAGTGTACTCCAGGGAGGAAAAATTTGGGGGTAAACAATTAATAGAGTCGATCGCGGAACATTATAAAATGACTTTA
GAACAAGCGACATTAGCAAAAGAGAATGGAGAATTACCTGAAGACTATGAAAAGGAAGTTTTAGAACCTTTTAAAGAGGA
TATATTGTTGCAAATCAAGCGTACCTTGCAGTTTTTTTATTCTACAAGTCAAGATGGGGATGTAGATCATATACTATTGG
CTGGAGGACTAGCCAAACTTCCAGGCTTGGTTTCATTAGTTCAAGAGCGATTAGGAATGAGTACAACCATTGCGAATCCA
TTTTCATATATGACTCCAGGGAAAATGATGAACTTGGATGCGGTAAATAATGATGCTCCATCACTTATGGTCGCTTGTGG
TCTGGCATTAAGGAATATTAGGTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Legionella pneumophila strain ERS1305867

70.538

99.718

0.703
  pilM Acinetobacter baumannii D1279779

44.193

99.718

0.441
  comM Acinetobacter nosocomialis M2

44.193

99.718

0.441
  comM Acinetobacter baylyi ADP1

43.343

99.718

0.432

Multiple sequence alignment