Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ELZ61_RS04745 Genome accession   NZ_LR134167
Coordinates   965245..966660 (+) Length   471 a.a.
NCBI ID   WP_126371844.1    Uniprot ID   -
Organism   Avibacterium volantium strain NCTC3438     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 960245..971660
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ELZ61_RS04715 (NCTC3438_00959) lgt 960513..961313 (-) 801 WP_126371834.1 prolipoprotein diacylglyceryl transferase -
  ELZ61_RS04720 (NCTC3438_00960) - 961323..962114 (-) 792 WP_126371836.1 sulfite exporter TauE/SafE family protein -
  ELZ61_RS04725 (NCTC3438_00961) rppH 962114..962731 (-) 618 WP_126371838.1 RNA pyrophosphohydrolase -
  ELZ61_RS04730 (NCTC3438_00962) - 963393..964022 (+) 630 WP_126371840.1 cation diffusion facilitator family transporter -
  ELZ61_RS04735 (NCTC3438_00963) ampD 964102..964656 (-) 555 WP_126371842.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ELZ61_RS10825 ppdD 964799..965232 (+) 434 Protein_916 prepilin peptidase-dependent pilin -
  ELZ61_RS04745 (NCTC3438_00965) pilB 965245..966660 (+) 1416 WP_126371844.1 GspE/PulE family protein Machinery gene
  ELZ61_RS04750 (NCTC3438_00966) - 966707..971638 (-) 4932 WP_126371846.1 exo-alpha-sialidase -

Sequence

Protein


Download         Length: 471 a.a.        Molecular weight: 53129.88 Da        Isoelectric Point: 5.8086

>NTDB_id=1118648 ELZ61_RS04745 WP_126371844.1 965245..966660(+) (pilB) [Avibacterium volantium strain NCTC3438]
MAEYALQPQMTVCAVDGKFFEIPPHLWQQNQQQKALLIRYFAVPLQENAQTLWLGIDDLNNLSACETFSFLTGKVVEPVL
LQGQDLKLLLQSLAPNVAEEPAVPDFYRPTEQIKTENVQEKADEPVIQLLDRLFEKALQLNASDIHLEPQREQLQIRFRI
DGVLQCQTPLSTQISQRLISRLKLLARLDISETRLPQDGRFHFKTTFSDVLDFRLSTLPTQYGEKAVLRLQKNKPVSLAF
SQLGMNAAQQKALQQALSQPQGLILVTGPTGSGKSITLYTALEWLNQSEKHILTAEDPIEIELPGIIQTQINPQIGLDFN
RLLRTFLRQDPDIIMLGEIRDAESAAIALRAAQTGHLVLSTLHTNNAHSAISRLLQLGIQQHEIESSLLLVIAQRLVRKI
CTHHTENCDCYQGYKGRCGIYQFLQPHFNNGEISYQTDYASLRESGLEKVKQGITDPAEVERVLGKLMESE

Nucleotide


Download         Length: 1416 bp        

>NTDB_id=1118648 ELZ61_RS04745 WP_126371844.1 965245..966660(+) (pilB) [Avibacterium volantium strain NCTC3438]
ATGGCGGAATATGCGTTACAGCCGCAAATGACGGTTTGTGCGGTAGATGGAAAATTTTTTGAAATTCCACCGCACTTATG
GCAGCAAAACCAACAGCAAAAAGCCCTGCTAATTCGCTATTTTGCTGTGCCATTGCAAGAAAATGCCCAAACGCTTTGGT
TAGGCATTGATGATTTGAATAACCTTTCTGCTTGCGAAACCTTTTCTTTTTTAACAGGAAAAGTGGTTGAACCTGTGTTG
TTGCAAGGGCAAGATTTGAAATTGCTCTTGCAAAGTCTTGCGCCAAATGTGGCGGAAGAACCTGCTGTACCCGATTTTTA
TCGCCCCACTGAGCAGATTAAAACGGAAAATGTGCAAGAAAAAGCCGATGAGCCAGTTATTCAACTCTTAGATCGGCTTT
TTGAAAAGGCGTTACAACTGAATGCTTCAGATATTCATTTAGAGCCACAGCGCGAGCAGTTGCAAATCCGCTTTCGTATT
GATGGCGTATTGCAGTGCCAGACGCCATTATCCACGCAAATTAGTCAGCGCTTAATTTCTCGCTTAAAATTGCTCGCTCG
CCTTGATATAAGCGAAACACGTTTGCCACAAGATGGACGTTTTCATTTTAAAACCACCTTTTCTGATGTGTTAGATTTTC
GCCTTTCCACCTTGCCCACCCAGTATGGCGAAAAAGCGGTGTTACGTTTGCAGAAAAACAAGCCTGTCAGCTTAGCGTTT
TCACAGTTGGGAATGAACGCAGCGCAGCAAAAGGCTTTGCAGCAAGCCCTTTCACAGCCTCAAGGCTTGATTTTAGTTAC
AGGCCCAACAGGAAGCGGAAAAAGTATTACCCTTTACACCGCACTTGAATGGTTGAATCAGTCGGAAAAGCATATTCTCA
CCGCCGAAGATCCCATTGAAATAGAACTGCCGGGCATTATCCAAACGCAAATTAATCCACAAATTGGGCTAGATTTTAAC
CGATTGTTACGCACATTTTTACGCCAAGATCCTGACATCATTATGCTTGGCGAAATTCGAGATGCAGAAAGTGCAGCGAT
TGCTTTGCGTGCTGCACAAACAGGGCATCTAGTGCTTTCTACGCTGCATACCAATAATGCTCATTCAGCAATTTCTCGCT
TATTGCAGCTCGGTATTCAGCAGCACGAAATTGAAAGCAGTTTGCTGCTCGTTATCGCCCAGCGTTTAGTGCGCAAAATT
TGTACGCATCACACCGAAAATTGCGATTGCTATCAAGGCTATAAAGGGCGTTGCGGAATTTATCAATTTTTACAACCGCA
CTTTAATAATGGGGAAATTTCCTATCAAACGGATTATGCTTCCTTACGAGAAAGCGGATTGGAAAAGGTAAAGCAAGGAA
TAACCGATCCCGCTGAAGTAGAAAGAGTGCTAGGGAAATTGATGGAGAGCGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(5-96)

Type II/IV secretion system protein (T2SSE)

(133-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

67.66

99.788

0.675
  pilB Haemophilus influenzae Rd KW20

67.66

99.788

0.675
  pilB Glaesserella parasuis strain SC1401

59.023

100

0.59
  pilB Acinetobacter baumannii D1279779

38.104

100

0.418
  pilB Vibrio cholerae strain A1552

40.166

100

0.412
  pilB Legionella pneumophila strain ERS1305867

37.476

100

0.41
  pilB Vibrio parahaemolyticus RIMD 2210633

39.876

100

0.41
  pilB Vibrio campbellii strain DS40M4

39.834

100

0.408
  pilB Acinetobacter baylyi ADP1

37.626

100

0.397
  pilF Neisseria gonorrhoeae MS11

38.365

100

0.389
  pilF Thermus thermophilus HB27

37.195

100

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.792

100

0.378

Multiple sequence alignment