Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   KKKWG1_RS09370 Genome accession   NZ_LN869922
Coordinates   1802852..1804120 (-) Length   422 a.a.
NCBI ID   WP_019389681.1    Uniprot ID   -
Organism   Kingella kingae strain KWG1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1797852..1809120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KKKWG1_RS09340 (KKKWG1_1902) - 1798059..1799252 (+) 1194 WP_019389685.1 acetate kinase -
  KKKWG1_RS09345 (KKKWG1_1903) - 1799329..1799949 (-) 621 WP_019389684.1 L-threonylcarbamoyladenylate synthase -
  KKKWG1_RS09350 (KKKWG1_1904) pncC 1799959..1800435 (-) 477 WP_003787789.1 nicotinamide-nucleotide amidase -
  KKKWG1_RS09355 (KKKWG1_1905) zapD 1800455..1801216 (-) 762 WP_019389683.1 cell division protein ZapD -
  KKKWG1_RS09360 (KKKWG1_1906) coaE 1801331..1801939 (-) 609 WP_003787784.1 dephospho-CoA kinase -
  KKKWG1_RS09365 (KKKWG1_1907) pilD 1801941..1802780 (-) 840 WP_341785704.1 A24 family peptidase Machinery gene
  KKKWG1_RS09370 (KKKWG1_1908) pilG 1802852..1804120 (-) 1269 WP_019389681.1 type II secretion system F family protein Machinery gene
  KKKWG1_RS09375 (KKKWG1_1909) pilF 1804167..1805852 (-) 1686 WP_003787777.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KKKWG1_RS09380 (KKKWG1_1910) lysA 1806038..1807267 (-) 1230 WP_099046166.1 diaminopimelate decarboxylase -
  KKKWG1_RS11730 (KKKWG1_1911) - 1807264..1807425 (-) 162 WP_003787775.1 lipoprotein -
  KKKWG1_RS09385 (KKKWG1_1912) cyaY 1807516..1807839 (+) 324 WP_099046165.1 iron donor protein CyaY -
  KKKWG1_RS09390 (KKKWG1_1913) pgeF 1807989..1808768 (+) 780 WP_285064162.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 46031.51 Da        Isoelectric Point: 10.1438

>NTDB_id=1114887 KKKWG1_RS09370 WP_019389681.1 1802852..1804120(-) (pilG) [Kingella kingae strain KWG1]
MAAKKPVKKAPVKKAAAAKPVKKEKGNRYQFEGRNLSTDSIVRGEVVAKNEDEARQKLVRRQIKVLQLTKMKKAKDKKIT
SADIAVFTRQLSTMMKAGLPLMQAFDIVAKGHSNASMTAMLMEIRNDVEQGEALGDSFAKHPKYFDKFYCNLVAAGEAGG
VLEGLLDKLATYKEKTEAIKKKVKSALTYPISIVVVAVVLVIVMMMFVLPEFGKVYESMGAKLPPLTQFMMDISNAFVQF
GWLIIAGLIGGVVALIQFHKRSPKFQKRVDAMLLKLPVFGDIVRKATIARWARTTATLFTAGVPLVEVLDSVAGAAGNII
YEEATREIRSKVNQGISLTSGMQATNLFPNMVVQMASIGEESGALDDMLNKTAEFYEDEVDAAVAMLSSLMEPIIMVVLG
GIIGTILVAMYLPLFNLGNVVG

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=1114887 KKKWG1_RS09370 WP_019389681.1 1802852..1804120(-) (pilG) [Kingella kingae strain KWG1]
ATGGCTGCAAAAAAACCTGTTAAAAAAGCACCTGTAAAAAAAGCAGCGGCTGCCAAGCCTGTTAAAAAAGAAAAAGGCAA
TCGCTACCAGTTTGAAGGTCGTAATTTAAGTACCGATTCAATCGTACGCGGCGAAGTGGTTGCCAAAAATGAAGACGAAG
CACGTCAGAAGTTAGTGCGTCGCCAAATTAAGGTTTTGCAACTGACCAAAATGAAAAAGGCAAAAGATAAGAAAATTACT
TCTGCCGACATTGCGGTGTTTACGCGCCAACTTTCTACCATGATGAAAGCAGGTTTGCCATTGATGCAAGCGTTTGATAT
CGTGGCAAAAGGTCATTCCAATGCATCTATGACAGCCATGCTGATGGAAATTCGCAACGATGTGGAACAAGGTGAAGCAT
TGGGCGATTCGTTTGCCAAACACCCTAAATATTTTGACAAATTCTATTGTAACTTGGTTGCCGCAGGTGAAGCAGGCGGT
GTATTGGAAGGCTTGTTAGACAAACTGGCAACCTATAAAGAAAAAACCGAAGCTATTAAGAAAAAAGTGAAATCGGCTTT
GACTTACCCAATTTCTATTGTGGTAGTGGCAGTCGTTTTGGTAATTGTGATGATGATGTTTGTATTGCCTGAGTTTGGTA
AGGTGTATGAAAGCATGGGGGCAAAATTGCCACCTCTCACACAGTTTATGATGGATATTTCTAATGCATTTGTTCAATTT
GGTTGGTTGATTATTGCAGGCTTGATTGGTGGAGTGGTTGCATTGATTCAATTTCATAAAAGATCACCTAAGTTCCAAAA
AAGAGTAGATGCTATGTTGTTAAAATTACCTGTATTTGGTGATATTGTCCGTAAAGCCACGATTGCACGTTGGGCACGTA
CAACCGCCACTTTGTTTACAGCGGGTGTACCATTGGTGGAAGTATTGGATTCGGTAGCAGGTGCCGCGGGTAATATTATT
TATGAAGAGGCCACTCGTGAAATTCGTTCCAAAGTAAACCAAGGTATTTCTTTGACATCGGGTATGCAGGCAACCAATTT
GTTCCCAAACATGGTAGTGCAAATGGCTTCCATTGGTGAAGAATCTGGCGCGTTGGACGATATGTTAAACAAAACTGCCG
AATTCTATGAAGACGAAGTCGATGCTGCGGTAGCCATGCTGTCGTCTTTGATGGAACCCATCATTATGGTGGTATTGGGT
GGCATTATTGGTACGATTCTGGTTGCGATGTACTTACCATTGTTCAACTTGGGTAACGTGGTAGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(87-210)

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

68

94.787

0.645
  pilG Neisseria meningitidis 44/76-A

67.25

94.787

0.637
  pilC Legionella pneumophila strain ERS1305867

48.995

94.313

0.462
  pilC Acinetobacter baylyi ADP1

45.337

91.469

0.415
  pilC Pseudomonas stutzeri DSM 10701

50.435

81.754

0.412
  pilC Acinetobacter baumannii D1279779

45.195

91.232

0.412
  pilC Vibrio cholerae strain A1552

37.26

98.578

0.367

Multiple sequence alignment