Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HALO_RS12870 Genome accession   NZ_LN813019
Coordinates   2784120..2785871 (+) Length   583 a.a.
NCBI ID   WP_050713503.1    Uniprot ID   A0A0D6EAC5
Organism   Halomonas sp. R57-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2779120..2790871
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HALO_RS12840 (HALO2582) - 2779506..2779964 (+) 459 WP_035579090.1 universal stress protein -
  HALO_RS12845 (HALO2583) - 2780022..2781515 (+) 1494 WP_035579089.1 MFS transporter -
  HALO_RS12855 (HALO2584) - 2781923..2782744 (-) 822 WP_050713501.1 inositol monophosphatase family protein -
  HALO_RS12860 (HALO2585) - 2782839..2783273 (-) 435 WP_035579085.1 anti-virulence regulator CigR family protein -
  HALO_RS12865 (HALO2586) - 2783406..2783900 (-) 495 WP_050713502.1 pilin -
  HALO_RS12870 (HALO2587) pilB 2784120..2785871 (+) 1752 WP_050713503.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HALO_RS12875 (HALO2588) pilC 2785875..2787107 (+) 1233 WP_035579080.1 type II secretion system F family protein Machinery gene
  HALO_RS12880 (HALO2589) pilD 2787156..2788001 (+) 846 WP_035579078.1 A24 family peptidase Machinery gene
  HALO_RS12885 (HALO2590) coaE 2788086..2788688 (+) 603 WP_035579076.1 dephospho-CoA kinase -
  HALO_RS12890 (HALO2591) yacG 2788723..2788956 (+) 234 WP_022523595.1 DNA gyrase inhibitor YacG -
  HALO_RS12895 (HALO2592) - 2788968..2789855 (+) 888 WP_035579074.1 DNA replication terminus site-binding protein -
  HALO_RS12900 (HALO2593) - 2789895..2790236 (-) 342 WP_035583345.1 hypothetical protein -

Sequence

Protein


Download         Length: 583 a.a.        Molecular weight: 63728.98 Da        Isoelectric Point: 6.5705

>NTDB_id=1114040 HALO_RS12870 WP_050713503.1 2784120..2785871(+) (pilB) [Halomonas sp. R57-5]
MSQPPSLSHAAARGGLRGIAQRLVKHDLLTGAQAATAETTASDLEISLLQHVIESGLVDPIKATLAAGWEYGLPVIDLET
VRLAALPPASDYPTKVLQSLNVLPLARHGHRLTVAVPYPATLTQLDELQIATGLGVEGILAPVDQITVALNSYLAQNERG
MLDELDDIDDVVSSLSIVQNNDGSEAPLLEESSQNSDDAPIVKFVNKILLDAIRRGASDIHFESYETLYRVRFRVDGMLT
EVARPPFAMRNRIAARLKIMAHLDIAERRLPQDGTIKIKLSRTRSLDFRVNSLPTVYGEKLVLRILDPTTAQLGIEQLGF
TDEQRGHYERALKQPQGMILVTGPTGSGKTVSLYTGINILNKVERNICTAEDPVEIKVAGINQVSVLPKIGLDFASALRA
FLRQDPDVVMVGEIRDLETAEIAVKAAQTGHLVLSTVHTNSAAETLTRLANMGVSSFNIASAVSLIIAQRLARKLCTYCK
EPIEIPHEALRKEGFSXAELHQSTTYKPVGCKRCTQGYKGRIGIYEVVPITEAMRQLIMRDANALDIDQQARKEGYPSLH
RSGLLKVMQGITSLEEVNRVSKE

Nucleotide


Download         Length: 1752 bp        

>NTDB_id=1114040 HALO_RS12870 WP_050713503.1 2784120..2785871(+) (pilB) [Halomonas sp. R57-5]
ATGAGCCAGCCCCCCTCGTTAAGTCACGCCGCCGCGCGTGGCGGCCTGCGCGGCATTGCCCAGCGCTTGGTTAAGCACGA
CTTGCTGACCGGCGCACAGGCCGCCACGGCAGAAACAACCGCGTCGGATTTGGAAATCTCGCTACTTCAGCATGTTATCG
AAAGTGGACTGGTCGACCCTATAAAAGCCACACTGGCCGCAGGCTGGGAGTATGGGTTGCCGGTCATTGACTTGGAGACT
GTGCGGCTTGCCGCCCTGCCCCCGGCCTCTGACTACCCCACCAAAGTGCTGCAGAGCCTTAACGTTCTGCCTTTAGCACG
GCACGGTCATCGACTCACGGTCGCGGTACCCTACCCGGCCACGTTGACGCAACTGGATGAATTGCAGATTGCCACGGGCC
TTGGCGTTGAGGGCATCCTTGCGCCCGTTGATCAAATCACCGTCGCCCTCAATAGCTACCTGGCGCAAAACGAGCGCGGC
ATGTTGGATGAGCTCGACGATATTGATGATGTGGTTAGCTCGTTGAGCATTGTTCAGAATAACGACGGTAGCGAGGCCCC
GCTGCTGGAAGAGTCTTCCCAAAATAGCGACGACGCGCCTATCGTCAAATTTGTGAATAAGATTCTGTTGGACGCTATCC
GCCGGGGCGCCTCCGATATCCATTTCGAGTCCTACGAAACACTCTACCGGGTGCGTTTCCGTGTTGACGGTATGCTAACA
GAAGTGGCTCGGCCTCCCTTCGCCATGCGCAACCGTATCGCGGCCCGTCTGAAAATCATGGCGCACCTCGATATTGCCGA
GCGCCGCTTACCCCAGGATGGAACGATAAAAATTAAGCTTTCCCGCACCCGCTCGCTGGATTTCCGGGTTAACTCACTGC
CTACCGTCTATGGCGAAAAGCTGGTGCTGCGGATTTTAGACCCCACCACAGCGCAGCTGGGTATTGAGCAATTAGGCTTC
ACCGACGAACAGCGTGGCCATTATGAACGAGCGCTAAAACAGCCCCAAGGCATGATCCTGGTCACCGGCCCCACCGGTAG
CGGCAAGACCGTTTCGCTGTATACCGGCATTAATATTCTTAATAAAGTTGAGCGCAATATATGCACCGCCGAAGATCCGG
TTGAGATCAAAGTCGCCGGCATCAACCAGGTGAGCGTATTACCTAAAATCGGCCTCGATTTTGCCAGCGCGCTCAGAGCG
TTTCTGCGCCAAGATCCTGATGTAGTCATGGTCGGGGAGATTCGCGACCTTGAGACAGCCGAAATTGCCGTTAAAGCAGC
CCAGACAGGACACTTGGTGCTCTCCACGGTACATACTAACTCCGCGGCAGAAACACTGACGCGACTGGCTAATATGGGCG
TCTCCTCGTTTAACATTGCCAGCGCCGTAAGTTTGATTATTGCTCAACGGCTGGCGCGCAAACTATGTACATACTGCAAA
GAGCCCATTGAGATTCCCCACGAAGCGCTGCGTAAAGAAGGCTTTAGCAKCGCAGAGCTCCACCAGTCGACTACTTACAA
ACCGGTCGGTTGCAAACGCTGCACCCAAGGCTACAAAGGCCGCATTGGTATTTATGAGGTAGTACCCATTACGGAAGCGA
TGCGCCAGTTGATTATGCGTGATGCCAATGCACTCGATATTGACCAACAGGCGCGCAAGGAGGGCTATCCAAGCCTTCAC
CGCAGCGGGTTATTAAAAGTCATGCAGGGTATTACCAGCCTGGAAGAGGTTAACCGAGTAAGCAAGGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-158)

Type II/IV secretion system protein (T2SSE)

(207-474)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50.88

97.427

0.496
  pilB Acinetobacter baylyi ADP1

49.147

100

0.494
  pilB Acinetobacter baumannii D1279779

50.352

97.427

0.491
  pilB Vibrio campbellii strain DS40M4

50.909

94.34

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

49.091

94.34

0.463
  pilB Vibrio cholerae strain A1552

51.663

87.65

0.453
  pilF Neisseria gonorrhoeae MS11

50

82.676

0.413
  pilB/pilB1 Synechocystis sp. PCC 6803

38.657

94.511

0.365
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.4

91.424

0.36

Multiple sequence alignment