Detailed information NTG1114042

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HALO_RS12880
• Genome accession: NZ_LN813019
• Coordinates: 2787156..2788001 (+)
• Length: 281 a.a.
• NCBI ID: WP_035579078.1
• Uniprot ID: A0A0D6E915
• Organism: Halomonas sp. R57-5
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2782156..2793001

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HALO_RS12860 (HALO2585)	-	2782839..2783273 (-)	435	WP_035579085.1	anti-virulence regulator CigR family protein	-
HALO_RS12865 (HALO2586)	-	2783406..2783900 (-)	495	WP_050713502.1	pilin	-
HALO_RS12870 (HALO2587)	pilB	2784120..2785871 (+)	1752	WP_050713503.1	type IV-A pilus assembly ATPase PilB	Machinery gene
HALO_RS12875 (HALO2588)	pilC	2785875..2787107 (+)	1233	WP_035579080.1	type II secretion system F family protein	Machinery gene
HALO_RS12880 (HALO2589)	pilD	2787156..2788001 (+)	846	WP_035579078.1	A24 family peptidase	Machinery gene
HALO_RS12885 (HALO2590)	coaE	2788086..2788688 (+)	603	WP_035579076.1	dephospho-CoA kinase	-
HALO_RS12890 (HALO2591)	yacG	2788723..2788956 (+)	234	WP_022523595.1	DNA gyrase inhibitor YacG	-
HALO_RS12895 (HALO2592)	-	2788968..2789855 (+)	888	WP_035579074.1	DNA replication terminus site-binding protein	-
HALO_RS12900 (HALO2593)	-	2789895..2790236 (-)	342	WP_035583345.1	hypothetical protein	-
HALO_RS12905 (HALO2594)	-	2790624..2791442 (+)	819	WP_231896712.1	helix-turn-helix transcriptional regulator	-
HALO_RS12910 (HALO2595)	-	2791532..2791906 (-)	375	WP_035579070.1	histone-like nucleoid-structuring protein, MvaT/MvaU family	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 30845.28 Da
• Isoelectric Point: 7.9082

>NTDB_id=1114042 HALO_RS12880 WP_035579078.1 2787156..2788001(+) (pilD) [Halomonas sp. R57-5]
MHHPPFILWLIVLLMGLCLGSFLNVVITRLPVMLMRGWRNEAREALELSAEHSPRFNLTTPGSMCPRCETPIAWHDNLPL
IGWIKRRGRCAACQTPISIQYPLVEIAGGLLAMAVLALHGLSAESVFVYGACLMLLVLAVIDFRTQLLPDVITLPLLWAG
LLFQLLFQPLMLQSAVIGAMVGYLSLWSFYWLFKLVTGKEGMGYGDFKLLAALGAWLGWSFLPLILLLSAGLGAIVGLVA
QACSPRLRGTPFPFGPFLALAGWVALLVGDEIMVLYLSLLM

Nucleotide

• Length: 846 bp

>NTDB_id=1114042 HALO_RS12880 WP_035579078.1 2787156..2788001(+) (pilD) [Halomonas sp. R57-5]
ATGCATCACCCTCCGTTTATTCTGTGGTTAATCGTTTTATTGATGGGACTATGCCTGGGCAGTTTTCTAAATGTGGTGAT
TACGCGCCTGCCGGTGATGCTGATGCGCGGCTGGCGTAACGAAGCCCGCGAAGCACTTGAGCTTAGCGCCGAACACTCAC
CACGCTTTAATTTGACTACGCCTGGCTCCATGTGCCCACGCTGCGAAACCCCTATAGCATGGCACGACAACCTACCGCTG
ATCGGCTGGATAAAGCGCCGCGGGCGCTGTGCTGCGTGCCAAACGCCTATTAGCATTCAATACCCGCTGGTAGAAATAGC
CGGTGGCTTACTGGCCATGGCAGTGCTAGCGCTCCACGGGCTTTCTGCAGAATCAGTCTTCGTTTATGGCGCCTGCCTGA
TGCTGTTAGTGCTCGCCGTGATCGACTTTCGCACCCAGCTACTGCCCGATGTCATCACCCTCCCCTTACTTTGGGCAGGG
TTACTGTTTCAGCTCCTCTTCCAGCCACTTATGCTACAGAGTGCCGTTATCGGCGCAATGGTAGGCTATCTTTCACTGTG
GAGTTTTTATTGGCTATTCAAACTGGTCACCGGCAAAGAGGGAATGGGCTATGGCGACTTTAAGCTCCTAGCAGCGCTAG
GCGCCTGGCTGGGATGGAGTTTTTTACCGCTGATTTTGCTACTTTCCGCAGGCCTTGGGGCCATTGTTGGACTGGTTGCC
CAAGCCTGTTCACCACGCTTGCGCGGCACACCGTTTCCCTTTGGTCCTTTTCTTGCCCTCGCGGGCTGGGTTGCGTTGCT
AGTCGGTGATGAAATAATGGTGCTATATCTCAGCCTGCTTATGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (14-116)

• Type IV leader peptidase family (Peptidase_A24) (132-238)

3D structure

• AlphaFold DB: A0A0D6E915

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.097	98.577	0.484
pilD	Acinetobacter baumannii D1279779	48	97.865	0.47
pilD	Acinetobacter nosocomialis M2	48	97.865	0.47
pilD	Vibrio campbellii strain DS40M4	45.07	100	0.456
pilD	Neisseria gonorrhoeae MS11	46.36	92.883	0.431