Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LFA_RS06980 Genome accession   NZ_LN614827
Coordinates   1720667..1722388 (+) Length   573 a.a.
NCBI ID   WP_045095546.1    Uniprot ID   A0A098G3D1
Organism   Legionella fallonii LLAP-10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1715667..1727388
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LFA_RS06955 (LFA_1557) - 1715688..1717901 (-) 2214 WP_157010306.1 hypothetical protein -
  LFA_RS06965 (LFA_1559) - 1718283..1718843 (-) 561 WP_045095543.1 hypoxanthine-guanine phosphoribosyltransferase -
  LFA_RS06970 (LFA_1560) - 1718953..1719279 (-) 327 WP_045095544.1 DUF1820 family protein -
  LFA_RS06975 (LFA_1561) - 1719414..1720145 (-) 732 WP_045095545.1 methyltransferase domain-containing protein -
  LFA_RS06980 (LFA_1563) pilB 1720667..1722388 (+) 1722 WP_045095546.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LFA_RS06985 (LFA_1564) pilC 1722393..1723613 (+) 1221 WP_045095547.1 type II secretion system F family protein Machinery gene
  LFA_RS06990 (LFA_1565) pilD 1723654..1724517 (+) 864 WP_045095548.1 A24 family peptidase Machinery gene
  LFA_RS06995 (LFA_1566) - 1724749..1724991 (+) 243 WP_045095549.1 hypothetical protein -
  LFA_RS07000 (LFA_1567) - 1724988..1725431 (+) 444 WP_065814366.1 hypothetical protein -
  LFA_RS07005 (LFA_1568) - 1725463..1725621 (+) 159 WP_157010307.1 hypothetical protein -
  LFA_RS07010 (LFA_1569) - 1725707..1726609 (-) 903 WP_084602133.1 branched-chain amino acid transaminase -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62818.31 Da        Isoelectric Point: 5.7245

>NTDB_id=1113613 LFA_RS06980 WP_045095546.1 1720667..1722388(+) (pilB) [Legionella fallonii LLAP-10]
MIAVDEHRLQGIGQLLVLEKLLDKTKAIEYSKLAAEEKTPLLQYLVKNNILSAEQIALTAAQSFGVPLLDITSIEIDSIP
VTLVNEKLIRRHAMIPLFTRGNNLYLATDDPSKQASLKEIQFHTGLNTNAIVVETDKLHAFIEQLLSEKENESLAGLGDG
ANELEGFEISPDDEEHDTETQTSVTDDAPIVKFVNKILLDAIKQGASDIHFEPYEKDYRIRYRQDGILREVATPPHSLSA
RITARIKVMSKLDISERRIPQDGGFKMKISKTRSIDFRVSTCPTSGGEKVVMRVLDPGSAKLGIEALGFSLIQKAHFIKA
IERPQGMILVTGPTGSGKTVSLYTALNILNTKEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIMV
GEIRDFETAEIAVKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTIIIAQRLARKLCDNCKELRDDYTNAGL
IELGFNEKDLTDIKLYKAVGCSQCTGGYRGRVGLFEVLPMTKALGQLIMSGANSLDILKVAQSEGMLTIFQSGLEKVKIG
ITTIEEVNRVTVE

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=1113613 LFA_RS06980 WP_045095546.1 1720667..1722388(+) (pilB) [Legionella fallonii LLAP-10]
ATGATTGCAGTGGATGAACATAGACTACAAGGGATTGGACAATTATTAGTCCTTGAGAAGCTTTTGGATAAAACAAAGGC
TATAGAATATAGCAAACTTGCCGCAGAAGAAAAAACTCCCTTATTGCAATATTTAGTTAAAAATAACATATTATCAGCAG
AACAAATTGCCCTCACCGCAGCTCAAAGCTTCGGTGTCCCCTTGCTCGATATTACCTCCATAGAGATTGACAGCATTCCT
GTAACCCTAGTCAATGAAAAATTGATTCGTCGTCATGCAATGATCCCGCTATTCACTAGAGGAAATAATTTATACTTAGC
GACCGATGATCCCAGCAAACAGGCATCTTTAAAAGAAATACAATTTCATACTGGATTAAATACCAATGCAATAGTAGTTG
AAACAGATAAGCTGCATGCATTTATAGAGCAATTATTATCTGAGAAAGAAAACGAAAGTCTTGCAGGATTAGGTGACGGG
GCTAATGAGTTAGAGGGTTTCGAAATCAGTCCTGACGATGAGGAACATGATACTGAAACTCAAACATCAGTTACAGACGA
CGCCCCTATTGTTAAATTCGTTAATAAAATTTTGCTAGATGCGATAAAACAAGGAGCATCGGATATTCATTTTGAGCCTT
ATGAAAAAGATTATCGTATTCGGTATAGACAAGACGGAATCTTGCGTGAGGTAGCAACACCTCCCCACAGCCTATCCGCA
CGTATTACTGCCCGTATCAAGGTAATGTCAAAATTGGATATTTCGGAACGAAGAATTCCCCAAGACGGTGGTTTTAAAAT
GAAAATCTCTAAAACACGATCTATAGATTTTAGAGTAAGTACCTGTCCTACTTCTGGTGGTGAAAAGGTTGTCATGAGGG
TTCTAGATCCAGGCTCTGCAAAATTAGGAATTGAAGCTCTAGGATTTAGCCTCATTCAAAAGGCACACTTTATTAAAGCA
ATTGAACGGCCACAAGGCATGATTCTAGTCACGGGCCCAACTGGTAGCGGTAAAACAGTAAGCTTGTATACCGCATTAAA
CATACTCAATACAAAAGAAGTTAATATTTCCACTGCAGAAGATCCAGTGGAAATTAAAGTACCTGGAATAAATCAAGTTA
ATATCAACCCCAAAGCTGGATTAACCTTCTCAGGTGCATTGCGTTCTTTTTTACGTCAAGACCCCGATATCATCATGGTT
GGAGAAATACGAGATTTTGAAACGGCAGAAATTGCGGTAAAAGCAGCTCAAACAGGACACTTAGTTCTATCGACCCTACA
CACCAATAGTGCAGCAGAAACATTAAACCGGCTGGTCAATATGGGAATACCTACTTTTAATATTGCCAGTTCCGTTACTA
TAATCATCGCTCAACGACTCGCTAGAAAGTTATGTGATAACTGTAAAGAACTACGAGATGATTATACCAATGCAGGCTTA
ATAGAGCTGGGATTTAATGAAAAAGACTTAACCGATATTAAATTATATAAAGCTGTGGGCTGCAGCCAATGCACGGGGGG
TTACCGAGGTCGCGTAGGATTATTTGAAGTACTTCCCATGACAAAAGCTCTTGGCCAGTTAATCATGTCAGGAGCGAACT
CTCTTGATATATTGAAAGTTGCCCAATCTGAAGGGATGCTTACAATATTCCAGTCAGGATTAGAAAAAGTGAAAATAGGT
ATTACAACCATCGAGGAGGTCAATCGGGTAACCGTTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A098G3D1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

83.566

99.825

0.834
  pilB Acinetobacter baumannii D1279779

51.232

99.127

0.508
  pilB Acinetobacter baylyi ADP1

50.263

99.651

0.501
  pilB Vibrio cholerae strain A1552

48.885

93.892

0.459
  pilB Vibrio parahaemolyticus RIMD 2210633

48.885

93.892

0.459
  pilF Neisseria gonorrhoeae MS11

46.797

98.08

0.459
  pilB Vibrio campbellii strain DS40M4

47.387

96.859

0.459
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.541

92.845

0.386
  pilF Thermus thermophilus HB27

39.179

93.543

0.366

Multiple sequence alignment