Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KHN80_RS16550 Genome accession   NZ_HG999365
Coordinates   3834337..3836064 (-) Length   575 a.a.
NCBI ID   WP_211914180.1    Uniprot ID   -
Organism   Xanthomonas arboricola pv. juglandis isolate Xanthomonas arboricola pv. juglandis CPBF 765 isolated from C. illinoinensis     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3833054..3850369 3834337..3836064 within 0


Gene organization within MGE regions


Location: 3833054..3850369
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHN80_RS16540 (XCY_003304) - 3833060..3833416 (+) 357 WP_211914179.1 hypothetical protein -
  KHN80_RS16545 (XCY_003305) - 3833416..3834153 (+) 738 WP_244860030.1 zeta toxin family protein -
  KHN80_RS16550 (XCY_003306) pilB 3834337..3836064 (-) 1728 WP_211914180.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KHN80_RS16555 (XCY_003307) pilA2 3836211..3836624 (-) 414 WP_211915341.1 pilin Machinery gene
  KHN80_RS16560 (XCY_003308) comP 3836752..3837174 (-) 423 WP_211914181.1 pilin Machinery gene
  KHN80_RS16565 (XCY_003309) pilC 3837503..3838765 (+) 1263 WP_026064718.1 type II secretion system F family protein Machinery gene
  KHN80_RS16570 (XCY_003310) - 3838772..3839635 (+) 864 WP_002812278.1 A24 family peptidase -
  KHN80_RS16575 (XCY_003311) coaE 3839649..3840260 (+) 612 WP_211914182.1 dephospho-CoA kinase -
  KHN80_RS22080 - 3840423..3844781 (+) 4359 WP_244860031.1 RHS repeat-associated core domain-containing protein -
  KHN80_RS16585 (XCY_003313) - 3844784..3845242 (+) 459 WP_211914183.1 hypothetical protein -
  KHN80_RS16590 (XCY_003314) - 3845641..3846042 (+) 402 WP_211914184.1 SymE family type I addiction module toxin -
  KHN80_RS16595 (XCY_003315) - 3846187..3846492 (+) 306 WP_211914185.1 helix-turn-helix transcriptional regulator -
  KHN80_RS16600 (XCY_003316) - 3846500..3847729 (+) 1230 WP_211914186.1 type II toxin-antitoxin system HipA family toxin -
  KHN80_RS16605 (XCY_003317) - 3847863..3849197 (-) 1335 WP_211914187.1 HAMP domain-containing sensor histidine kinase -
  KHN80_RS16610 (XCY_003318) - 3849190..3849867 (-) 678 WP_006448355.1 response regulator transcription factor -
  KHN80_RS16615 (XCY_003319) - 3849899..3850369 (-) 471 WP_244860033.1 hypothetical protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62504.74 Da        Isoelectric Point: 5.4464

>NTDB_id=1112690 KHN80_RS16550 WP_211914180.1 3834337..3836064(-) (pilB) [Xanthomonas arboricola pv. juglandis isolate Xanthomonas arboricola pv. juglandis CPBF 765 isolated from C. illinoinensis]
MNVANLVGITGIARRLVQDGVLDEVNARAAMDQAALAKVPLPQWFAEKKLVSAAQLAAANAVEFGMPLMDVSVFDASQNA
VKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSLGDDD
DDMGDLDVSSGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVKL
SQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQKLFL
DAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDPDII
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKAPLPEHA
LLAEGFTAEQMAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQRIGIRDLRQSALVKAA
HGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=1112690 KHN80_RS16550 WP_211914180.1 3834337..3836064(-) (pilB) [Xanthomonas arboricola pv. juglandis isolate Xanthomonas arboricola pv. juglandis CPBF 765 isolated from C. illinoinensis]
ATGAACGTCGCCAATCTAGTCGGAATCACAGGGATAGCGCGACGGTTAGTTCAAGATGGTGTACTTGACGAAGTTAATGC
TCGAGCAGCAATGGATCAGGCTGCGTTGGCTAAGGTTCCACTGCCACAGTGGTTTGCTGAGAAGAAGCTAGTCTCAGCCG
CTCAGCTGGCTGCAGCCAATGCCGTCGAGTTCGGCATGCCGCTGATGGACGTGTCGGTCTTTGACGCAAGCCAGAACGCA
GTCAAGCTGGTCAGTGAAGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTTGTTCGTGGG
GGTGAGCAACCCAACCCAGACCAGGGCGCTGGACGATATCAAGTTCCACACCAATCTGGTGGTGGAGCCGATCCTGGTCG
ATGAGGATCAGATCCGTCGAACATTGGAGCAGTGGCAAGCCAGCAATGCGGCGCTCGGCTCGTCGCTCGGTGACGACGAT
GACGACATGGGGGATCTGGACGTCTCCTCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGCCAAGGG
CGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACATCCATTTCG
AGCCGTACGAAGACGATTACCGGGTGCGGCTGCGCATCGATGGCCTGCTGAAGAATGTGGCCAAGGCGCCGGTGAAGTTG
AGCCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGACGGGCGCAT
CAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGTACCTTGCCGACCCTGTTCGGCGAGAAGGTTGTGC
TGCGTATCCTGGACGCCAGTGCGGCCAAGCTGGGGATCGAGAAGCTGGGCTACGAGGCGGATCAGCAGAAGCTGTTCCTG
GATGCGATTCACAAGCCCTACGGGATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGTTGTATACGGC
GCTGGGCATCCTTAACGATGAGACGCGCAATATTTCAACGGCCGAGGATCCGGTCGAAATCCGCTTGCCTGGTGTCAATC
AGGTGCAGCAAAACGTCAAGCGCGGCATGACGTTCGCTGCGGCGTTGCGCTCGTTCCTGCGCCAGGATCCGGACATCATC
ATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGTGTTGTCGAC
GCTGCATACCAATGATGCGCCGCAGACCATCGCGCGTCTGATGAACATGGGCATTGCGCCGTACAACATCACCTCGTCGG
TGACCTTGGTCATTGCACAGCGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGGCACCGCTGCCGGAGCACGCG
CTGTTGGCCGAAGGGTTCACCGCAGAGCAGATGGCTGCCGGAATCGAACTGTACGAGGCGGTGGGCTGCGACGAATGCAC
CGAAGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAAATCGGTGCGATCGTGCTGGAAGGCG
GCAACGCGATGCAGATCGCCGAGGCGGCGCAGAGGATCGGCATCCGCGATCTACGCCAGTCGGCGCTGGTCAAGGCGGCG
CATGGGGTGACGAGCCTGGCCGAGATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-465)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.634

98.783

0.55
  pilB Acinetobacter baylyi ADP1

54.93

98.783

0.543
  pilB Legionella pneumophila strain ERS1305867

52.021

98.957

0.515
  pilB Vibrio cholerae strain A1552

48.432

99.826

0.483
  pilF Neisseria gonorrhoeae MS11

48.415

98.783

0.478
  pilB Vibrio campbellii strain DS40M4

45.343

98.957

0.449
  pilB Vibrio parahaemolyticus RIMD 2210633

45.088

99.13

0.447
  pilF Thermus thermophilus HB27

40.192

90.435

0.363
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.171

92.348

0.362

Multiple sequence alignment