Detailed information NTG1112609

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: KHO07_RS04260
• Genome accession: NZ_HG995459
• Coordinates: 919565..921265 (-)
• Length: 566 a.a.
• NCBI ID: WP_150734768.1
• Uniprot ID: A0A5E7M2X0
• Organism: Pseudomonas sp. PP3
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 914565..926265

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KHO07_RS04230	-	915443..915685 (-)	243	WP_150734763.1	DUF2790 domain-containing protein	-
KHO07_RS04235	-	915936..916625 (+)	690	WP_150734764.1	energy-coupling factor ABC transporter permease	-
KHO07_RS04240	yacG	916654..916854 (-)	201	WP_056855639.1	DNA gyrase inhibitor YacG	-
KHO07_RS04245	coaE	916851..917474 (-)	624	WP_212626538.1	dephospho-CoA kinase	-
KHO07_RS04250	pilD	917471..918343 (-)	873	WP_212626539.1	A24 family peptidase	Machinery gene
KHO07_RS04255	pilC	918345..919562 (-)	1218	WP_212626540.1	type II secretion system F family protein	Machinery gene
KHO07_RS04260	pilB	919565..921265 (-)	1701	WP_150734768.1	type IV-A pilus assembly ATPase PilB	Machinery gene
KHO07_RS04265	pilA	921491..921910 (+)	420	WP_075948424.1	pilin	Machinery gene
KHO07_RS04270	-	922565..924589 (+)	2025	Protein_840	DUF3274 domain-containing protein	-

Sequence

Protein

• Length: 566 a.a.
• Molecular weight: 62664.69 Da
• Isoelectric Point: 6.3515

>NTDB_id=1112609 KHO07_RS04260 WP_150734768.1 919565..921265(-) (pilB) [Pseudomonas sp. PP3]
MNDIALSGLAKQLILAELLTDKSAQQAYQQAQRNRMSLVSYLVQNKLVKSRQVAEIASEHFGMALLDLHALDKDMQPKGL
VSEKLVRQHHALPLWRRGNKLFVGVSDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFDNPGTGLEETADVDLDG
LDIEATHDKSQDAIGGQEADDAPVVRFVHKMLLDAIRSGSSDLHFEPYEKHYRVRMRTDGVLHEVARPPIQLANRIAARL
KVMASLDISERRRPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPASAQMGIDALGYEPDQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGMDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAQTLTRLHNMGIQGFNIATSVSLIIAQRLARKLCSHCRVPVEIPRETLLKEGFPE
ERIGTFTIYGPVGCDHCNGGYKGRVGIYEVVKNTADLQRLIMAEGNALQIDDQMRKDGFNDLRTSGLLKAMQGITSLEEI
NRVTKD

Nucleotide

• Length: 1701 bp

>NTDB_id=1112609 KHO07_RS04260 WP_150734768.1 919565..921265(-) (pilB) [Pseudomonas sp. PP3]
ATGAATGACATCGCCCTCAGCGGTCTGGCCAAGCAGTTGATCCTGGCCGAGCTGCTTACTGACAAAAGCGCGCAACAGGC
GTACCAGCAAGCCCAACGCAACCGTATGTCCCTGGTCAGTTACCTGGTGCAGAACAAACTGGTGAAAAGCCGCCAGGTCG
CCGAGATCGCCTCGGAGCACTTCGGCATGGCTCTGCTGGACCTGCATGCGTTGGACAAGGACATGCAGCCCAAGGGGCTG
GTCAGCGAGAAGCTCGTGCGCCAGCATCACGCCCTGCCCCTCTGGCGCCGTGGCAACAAGTTGTTCGTTGGGGTTTCCGA
CCCGACCAACCATCAGGCCATCAACGACATCCAGTTCAGCACCGGGCTCACCACCGAAGCGATCCTGGTCGAAGACGACA
AGCTCAGCGATGCCATCGAGAAATTCTTCGACAACCCTGGCACTGGCCTGGAAGAAACCGCCGACGTCGACCTCGATGGC
CTGGACATCGAGGCCACCCATGACAAAAGCCAGGACGCTATCGGCGGTCAGGAGGCCGATGACGCCCCGGTGGTGCGTTT
CGTCCATAAAATGCTGCTCGACGCGATCAGGAGCGGTTCTTCCGACCTGCATTTCGAACCCTACGAAAAGCACTACCGCG
TGCGCATGCGTACCGATGGTGTGTTGCATGAAGTCGCCAGGCCGCCGATCCAGCTGGCCAACCGCATCGCCGCACGCCTG
AAAGTCATGGCCAGCCTCGACATCTCCGAACGTCGCAGGCCCCAGGACGGCCGGATCAAGATGCGCCTGTCAAAAAGCAA
GTCCATCGACTTTCGGGTCAATACCTTGCCCACTTTGTGGGGCGAGAAAGTGGTGATCCGGATTCTCGACCCCGCCAGCG
CGCAAATGGGCATCGACGCCCTTGGGTACGAGCCCGACCAGAAAGACCTGTACATGGCGGCCCTGAAACAGCCGCAAGGC
ATGATTCTGGTGACCGGCCCCACCGGCTCGGGCAAAACCGTGTCGCTCTACACCGGGCTGAACATTCTCAATACCGTGGA
CATCAACATTTCGACCGCCGAAGACCCGGTCGAAATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAAGCAGG
GCATGGATTTCGCCCAGGCGCTGCGCTCGTTCCTGCGTCAGGATCCGGACGTGATCATGGTCGGCGAAATCCGCGATCTC
GAAACCGCCGAAATCGCCATCAAGGCTGCCCAGACCGGGCACCTGGTACTGTCGACCCTGCACACCAACAGCGCCGCGCA
AACCCTGACCCGCCTGCACAACATGGGCATCCAGGGCTTCAATATCGCCACCTCCGTCAGCCTGATCATCGCCCAGCGCC
TGGCGCGCAAACTGTGCAGCCATTGCCGCGTGCCGGTGGAAATTCCCCGCGAAACGTTGCTAAAGGAAGGTTTTCCCGAG
GAACGCATCGGCACCTTCACGATCTATGGACCGGTCGGCTGCGATCACTGCAACGGCGGCTACAAAGGACGAGTGGGGAT
TTATGAAGTGGTGAAGAACACGGCGGACCTGCAACGGCTGATCATGGCTGAAGGCAACGCACTGCAAATCGATGACCAGA
TGCGTAAGGACGGCTTTAACGACCTGCGCACCTCAGGACTGCTCAAAGCCATGCAGGGCATCACCAGCCTGGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (42-147)

• Type II/IV secretion system protein (T2SSE) (190-457)

3D structure

• AlphaFold DB: A0A5E7M2X0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	57.848	100	0.58
pilB	Acinetobacter baumannii D1279779	57.447	99.647	0.572
pilB	Legionella pneumophila strain ERS1305867	54.947	100	0.549
pilB	Vibrio cholerae strain A1552	50.536	98.94	0.5
pilF	Neisseria gonorrhoeae MS11	50.266	99.47	0.5
pilB	Vibrio campbellii strain DS40M4	49.123	100	0.495
pilB	Vibrio parahaemolyticus RIMD 2210633	49.46	98.233	0.486
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.474	93.993	0.371
pilF	Thermus thermophilus HB27	36.749	100	0.367