Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   P3C48_RS16615 Genome accession   NZ_HG992341
Coordinates   3882634..3884379 (-) Length   581 a.a.
NCBI ID   WP_275548467.1    Uniprot ID   A0A8D6VF39
Organism   Xanthomonas arboricola pv. corylina strain CFBP 1159     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3877634..3889379
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3C48_RS16600 (CFBP1159_32840) sucC 3877819..3878988 (-) 1170 WP_016901264.1 ADP-forming succinate--CoA ligase subunit beta -
  P3C48_RS16605 (CFBP1159_32850) - 3879220..3880833 (+) 1614 WP_016901263.1 HAMP domain-containing sensor histidine kinase -
  P3C48_RS16610 (CFBP1159_32860) pilR 3881026..3882420 (+) 1395 WP_047127086.1 sigma-54 dependent transcriptional regulator Regulator
  P3C48_RS16615 (CFBP1159_32880) pilB 3882634..3884379 (-) 1746 WP_275548467.1 type IV-A pilus assembly ATPase PilB Machinery gene
  P3C48_RS16620 - 3884411..3884815 (-) 405 WP_275548562.1 pilin -
  P3C48_RS16625 - 3884961..3885401 (-) 441 WP_104613411.1 pilin -
  P3C48_RS16630 (CFBP1159_32890) pilC 3885748..3887004 (+) 1257 WP_275548358.1 type II secretion system F family protein Machinery gene
  P3C48_RS16635 (CFBP1159_32900) - 3887011..3887874 (+) 864 WP_275548357.1 A24 family peptidase -
  P3C48_RS16640 (CFBP1159_32910) coaE 3887888..3888511 (+) 624 WP_275548563.1 dephospho-CoA kinase -
  P3C48_RS16645 (CFBP1159_32920) - 3888611..3888766 (+) 156 Protein_3260 SymE family type I addiction module toxin -

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 62930.04 Da        Isoelectric Point: 6.0922

>NTDB_id=1112349 P3C48_RS16615 WP_275548467.1 3882634..3884379(-) (pilB) [Xanthomonas arboricola pv. corylina strain CFBP 1159]
MNAGPATNNINLVGITGIARRLVQDGALDEVSARSAMHQAANAKVPLPQWFAEKKLVSAAQLASANAVEFGMPLMDVSVF
DASQNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGS
SLGDDDEGMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVA
KAPVKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEAD
QQKLFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLR
QDPDIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKS
TLPEHALLAEGFTPAQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQSIGIRDLRQS
ALVKAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=1112349 P3C48_RS16615 WP_275548467.1 3882634..3884379(-) (pilB) [Xanthomonas arboricola pv. corylina strain CFBP 1159]
ATGAATGCTGGGCCTGCCACTAACAACATTAATCTCGTAGGTATAACCGGCATTGCCCGGCGATTGGTCCAAGACGGTGC
TCTAGACGAAGTCAGTGCTCGCTCCGCCATGCATCAGGCAGCCAACGCAAAAGTTCCTTTGCCGCAATGGTTCGCTGAAA
AAAAGTTGGTGTCGGCAGCGCAACTCGCCTCTGCCAATGCGGTTGAGTTTGGTATGCCCCTGATGGACGTTTCTGTCTTT
GATGCCAGCCAGAACGCAGTCAAGCTTGTCAGTGAAGAGTTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGG
CAACCGGTTATTCGTGGGGGTGAGCAACCCGACCCAGACTAGGGCGCTGGACGACATCAAGTTTCATACCAACCTGGTGG
TCGAGCCGATCCTGGTCGACGAAGACCAGATCCGTCGCACCTTGGAGCAATGGCAGGCCAGCAATGCGGCGCTTGGCTCG
TCGCTCGGGGACGATGATGAGGGGATGGGCGACCTGGACGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTC
CGGGGTCGATGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAG
CCTCCGACATCCATTTCGAGCCCTATGAAGACGACTACCGGGTGCGCTTGCGCATCGATGGGCTGCTGAAGAATGTGGCC
AAGGCACCGGTGAAGCTGAACCAGCGCATCGCGGCGCGTCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGT
GCCACAGGACGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACCTTGCCGACGTTGT
TCGGCGAGAAGGTGGTGCTGCGTATCCTTGACGGCAGCGCGGCCAAGCTGGGCATCGAGAAGCTGGGCTACGAGGCGGAT
CAGCAGAAGCTGTTCCTGGAGGCGATCCACAAACCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGAC
GGTGTCGCTGTACACGGCACTGGGCATCCTCAACGACGAGACGCGCAATATCTCCACGGCCGAGGACCCGGTGGAAATCC
GTTTGCCCGGCGTCAATCAGGTGCAGCAGAACAACAAGCGCGGCATGACTTTCGCCGCGGCGCTGCGTTCGTTCCTGCGC
CAGGACCCGGACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCCATCAAGGCCGCGCAGACCGG
TCACATGGTGCTGTCCACGCTGCATACCAACGATGCGCCACAGACCATCGCGCGTTTGATGAATATGGGCATCGCGCCGT
ACAACATCACCTCGTCGGTGACCCTGGTGATCGCGCAGCGTCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGCAAATCG
ACCTTGCCCGAGCATGCGTTGCTGGCCGAAGGCTTCACGCCGGCGCAGATCGCTGCCGGGATCGAGCTGTACGAAGCGGT
CGGTTGCGACGAGTGCACCGAAGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGCG
CGATCGTGCTGGAAGGCGGTAACGCCATGCAGATTGCCGAGGCGGCACAGAGTATCGGCATCCGCGACCTGCGTCAGTCG
GCGCTGGTCAAGGCCGCGCACGGGGTGACCAGCCTGGCCGAGATCAACCGGGTGACGAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-156)

Type II/IV secretion system protein (T2SSE)

(204-471)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

97.762

0.547
  pilB Acinetobacter baylyi ADP1

55.282

97.762

0.54
  pilB Legionella pneumophila strain ERS1305867

52.548

97.935

0.515
  pilB Vibrio cholerae strain A1552

48.527

99.312

0.482
  pilF Neisseria gonorrhoeae MS11

48.063

97.762

0.47
  pilB Vibrio campbellii strain DS40M4

45.87

97.935

0.449
  pilB Vibrio parahaemolyticus RIMD 2210633

46.429

96.386

0.448

Multiple sequence alignment