Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LOK40_RS15515 Genome accession   NZ_HG992336
Coordinates   3641788..3643524 (-) Length   578 a.a.
NCBI ID   WP_127159907.1    Uniprot ID   -
Organism   Xanthomonas arboricola strain 1311a isolate 1311a     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3636788..3648524
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOK40_RS15500 (XA1311A_30300) sucC 3636945..3638114 (-) 1170 WP_039519379.1 ADP-forming succinate--CoA ligase subunit beta -
  LOK40_RS15505 (XA1311A_30310) - 3638346..3639959 (+) 1614 WP_016901263.1 HAMP domain-containing sensor histidine kinase -
  LOK40_RS15510 (XA1311A_30320) pilR 3640152..3641546 (+) 1395 WP_127159848.1 sigma-54 dependent transcriptional regulator Regulator
  LOK40_RS15515 (XA1311A_30330) pilB 3641788..3643524 (-) 1737 WP_127159907.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LOK40_RS15520 (XA1311A_30340) - 3643757..3644206 (-) 450 WP_127159847.1 pilin -
  LOK40_RS15525 (XA1311A_30350) pilC 3644582..3645841 (+) 1260 WP_127159846.1 type II secretion system F family protein Machinery gene
  LOK40_RS15530 (XA1311A_30360) - 3645848..3646711 (+) 864 WP_039519369.1 A24 family peptidase -
  LOK40_RS15535 (XA1311A_30370) coaE 3646725..3647348 (+) 624 WP_127159845.1 dephospho-CoA kinase -
  LOK40_RS15540 - 3647448..3647603 (+) 156 Protein_3049 SymE family type I addiction module toxin -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62720.83 Da        Isoelectric Point: 5.6063

>NTDB_id=1112299 LOK40_RS15515 WP_127159907.1 3641788..3643524(-) (pilB) [Xanthomonas arboricola strain 1311a isolate 1311a]
MSTAASVNLVGITGIARRLVQDGALDEAIARAAMEQATTAKVPLPQWFSEKKLVTASQLAAANAVEFGMPLMDVSAFDAS
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEEQIRRTLEQWQASNASFGSSLG
SDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPAQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQSIGIRDLRQSALV
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1112299 LOK40_RS15515 WP_127159907.1 3641788..3643524(-) (pilB) [Xanthomonas arboricola strain 1311a isolate 1311a]
ATGAGCACAGCCGCTTCTGTCAATCTGGTTGGGATTACGGGCATCGCCCGTCGTCTAGTGCAGGACGGCGCACTAGACGA
AGCTATAGCACGTGCTGCAATGGAGCAGGCAACCACAGCCAAGGTGCCTCTGCCGCAGTGGTTCTCCGAAAAAAAACTCG
TGACTGCTTCACAGTTGGCTGCGGCCAACGCAGTTGAGTTCGGCATGCCATTGATGGATGTGTCTGCGTTCGACGCCAGC
CAGAATGCAGTCAAGTTGGTCAGTGAGGAGTTGCTCCAGAAATACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTTGGGGTAAGCAACCCAACCCAGACCCGGGCGCTGGACGACATCAAGTTCCACACCAATCTGGTGGTCGAGCCGA
TCCTGGTCGACGAGGAACAGATCCGTCGCACTTTGGAACAGTGGCAGGCAAGCAATGCTTCGTTTGGTTCGTCGCTAGGT
AGCGATGACGAGGAAATGGGCGACCTGGACGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGTGACGACACGCCGGTGGTGAAGTTTGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACA
TCCATTTCGAGCCCTATGAAGACGACTACCGGGTGCGGCTGCGCATCGATGGGCTGCTGAAGAATGTGGCCAAGGCACCG
GTGAAGCTGAACCAGCGCATCGCGGCGCGTCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGGGTGAGCACCTTGCCGACGTTGTTCGGCGAAA
AGGTGGTGTTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGAGAAGCTGGGCTACGAGGCGGACCAGCAAAAG
CTGTTCCTGGAGGCAATCCATAAGCCGTACGGGATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTACACGGCACTGGGCATCCTCAACGACGAAACGCGCAACATCTCCACCGCCGAGGACCCGGTGGAAATCCGCCTGCCCG
GCGTGAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGTCAGGATCCG
GACATCATCATGGTCGGCGAGATCCGCGACCTGGAGACCGCCGAGATCGCGATCAAGGCCGCGCAGACCGGTCACATGGT
GCTGTCCACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGTCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCTCGTCGGTGACCCTGGTGATCGCGCAGCGTCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGCAAATCGACCTTGCCC
GAGCATGCGTTGCTGGCGGAAGGCTTCACGCCGGCACAGATCGCTGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
CGAGTGTACCGAAGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAAGGCGGTAACGCGATGCAGATTGCCGAGGCGGCACAGAGTATCGGCATCCGCGACCTGCGCCAGTCGGCGTTGGTC
AAGGCCGCGCACGGGGTGACCAGCCTGGCCGAGATCAACCGAGTGACGAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

98.27

0.55
  pilB Acinetobacter baylyi ADP1

55.458

98.27

0.545
  pilB Legionella pneumophila strain ERS1305867

51.845

98.443

0.51
  pilB Vibrio cholerae strain A1552

50

95.848

0.479
  pilF Neisseria gonorrhoeae MS11

48.239

98.27

0.474
  pilB Vibrio parahaemolyticus RIMD 2210633

46.429

96.886

0.45
  pilB Vibrio campbellii strain DS40M4

45.518

98.443

0.448
  pilB/pilB1 Synechocystis sp. PCC 6803

35.961

100

0.379

Multiple sequence alignment