Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PKB_RS26350 Genome accession   NZ_HG322950
Coordinates   5639086..5641173 (-) Length   695 a.a.
NCBI ID   WP_043255934.1    Uniprot ID   -
Organism   Pseudomonas knackmussii B13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5634086..5646173
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PKB_RS26335 (PKB_5344) - 5635721..5637394 (-) 1674 WP_043255930.1 AAA family ATPase -
  PKB_RS26340 (PKB_5345) aroB 5637401..5638507 (-) 1107 WP_043255932.1 3-dehydroquinate synthase -
  PKB_RS26345 (PKB_5346) aroK 5638559..5639077 (-) 519 WP_043257824.1 shikimate kinase AroK -
  PKB_RS26350 (PKB_5347) pilQ 5639086..5641173 (-) 2088 WP_043255934.1 type IV pilus secretin PilQ Machinery gene
  PKB_RS26355 (PKB_5348) pilP 5641231..5641755 (-) 525 WP_043255936.1 type 4a pilus biogenesis lipoprotein PilP -
  PKB_RS26360 (PKB_5349) pilO 5641752..5642432 (-) 681 WP_052355394.1 type 4a pilus biogenesis protein PilO -
  PKB_RS26365 (PKB_5350) pilN 5642429..5643028 (-) 600 WP_043255938.1 type 4a pilus biogenesis protein PilN -
  PKB_RS26370 (PKB_5351) pilM 5643028..5644092 (-) 1065 WP_043255941.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 74942.99 Da        Isoelectric Point: 5.5504

>NTDB_id=1111938 PKB_RS26350 WP_043255934.1 5639086..5641173(-) (pilQ) [Pseudomonas knackmussii B13]
MNRTMTRLGVALLVGLFAPHLLAADLQSLDVASLPGDRVELKLGFDQPVVAPRGYTIEQPARIALDLPGVQNKLGSKNRE
LGVGNARSVTVVEAKDRTRLIINLGTLVPYSTRTQGNNLFILVGQGTGASAPVATAAPAATSYASSARPAQPKPYIPAGK
AIRNIDFQRGDQGEGNVVIDLSDPGVSPDIQEQGGKIRLNFPKTQLPESLRVRLDVKDFATPVQFVNASEAGGNATINIE
PSGLFDYLVYQTDNRMTVSIKPLSAGDAEKRKKDTFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLR
LQNVPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKASDIAKLFQSVTG
SATGRGASAATANTDNRGSITVDDRTNSIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEASVDYTKELGVNWSGSL
SNRGNWNVSGGTDSTSQTFVDLGVSTTTPTSGIGIGFVTNNTVLDLQLNAMEKTGNGEVVSQPKVVTSDKETAKILKGTE
VPYQEASSSGATSVSFKEAALSLEVTPQITPDNRIIMQVKVNKDEPDYQNALLGVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQTKTTAKVPFLGDVPVVGRLFKNDYVTDTKNELLVFLTPRIMNNQAIAIDR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=1111938 PKB_RS26350 WP_043255934.1 5639086..5641173(-) (pilQ) [Pseudomonas knackmussii B13]
ATGAACAGAACGATGACCCGCCTGGGCGTTGCCCTGCTGGTAGGCCTTTTTGCACCGCATCTGCTTGCGGCCGACCTGCA
GAGCCTTGACGTGGCCTCGCTGCCGGGCGATCGCGTGGAGCTCAAGCTCGGCTTCGACCAGCCAGTGGTGGCGCCGCGCG
GCTATACCATCGAGCAACCCGCGCGTATCGCGCTGGATCTGCCTGGTGTGCAGAACAAACTGGGCAGCAAGAACCGCGAG
TTGGGCGTGGGTAACGCCCGCAGCGTGACGGTTGTCGAGGCCAAGGATCGAACCCGGTTGATCATCAACTTGGGCACGCT
GGTGCCGTATTCGACCCGGACCCAGGGCAACAATCTGTTCATTCTCGTCGGCCAGGGCACTGGAGCTTCCGCACCCGTGG
CCACTGCTGCGCCAGCGGCGACCAGCTATGCCTCTTCGGCTCGTCCGGCGCAGCCCAAGCCTTACATTCCGGCCGGCAAG
GCGATTCGCAACATCGACTTCCAGCGCGGTGATCAGGGTGAAGGCAATGTCGTCATCGACCTCTCCGATCCTGGTGTCAG
CCCGGATATTCAGGAGCAAGGCGGCAAGATTCGCCTGAATTTCCCCAAGACCCAGCTTCCTGAAAGCCTGCGTGTGCGCC
TGGACGTGAAGGACTTCGCCACTCCGGTGCAGTTCGTCAACGCTTCGGAAGCGGGTGGAAACGCCACCATCAACATCGAG
CCGAGCGGTCTGTTCGATTATCTGGTGTATCAGACTGACAACCGCATGACCGTGAGCATCAAGCCGCTCAGTGCCGGTGA
TGCCGAGAAGCGTAAGAAGGACACCTTCGCCTACACCGGCGAGAAGCTGTCGCTGAACTTCCAGGACATCGACGTACGCT
CCGTCCTGCAGCTTATCGCAGACTTCACCGACCTGAACCTGGTGGCTTCCGATACTGTGCAGGGCAACATCACCCTGCGC
CTGCAGAACGTGCCCTGGGACCAGGCGCTGGATCTGGTGCTCAAGACCAAGGGCCTGGATAAGCGCAAGATCGGCAACGT
GCTTCTGGTGGCGCCGGCTGACGAGATCGCCGCTCGCGAGCGCCAGGAGCTGGAAGCGCAGAAACAGATTGCCGAACTGG
CGCCCCTGCGTCGTGAGTTGATCCAGGTGAACTACGCCAAAGCGTCTGATATTGCCAAGCTGTTCCAGTCGGTCACCGGT
AGCGCTACCGGTCGTGGTGCTTCGGCCGCGACCGCGAACACCGATAACCGCGGTTCGATCACCGTGGATGATCGCACCAA
CTCGATCATCGCCTATCAGACTCAGGACCGCTTGGATGAGCTGCGCCGCATCGTTTCGCAGCTCGACATTCCTGTGCGCC
AGGTGATGATCGAGGCTCGTATCGTCGAGGCGAGCGTCGATTACACCAAGGAGCTGGGTGTGAACTGGAGCGGATCGCTG
AGCAATCGTGGCAACTGGAACGTTTCCGGTGGCACCGACAGCACCTCGCAGACCTTCGTTGACCTGGGCGTGAGCACTAC
TACTCCGACGTCCGGTATCGGCATCGGCTTCGTGACCAACAACACCGTTCTCGATCTTCAGCTCAACGCGATGGAAAAGA
CCGGTAACGGTGAAGTGGTATCGCAGCCCAAGGTGGTCACCTCGGACAAGGAAACCGCGAAGATCCTGAAAGGTACCGAG
GTTCCGTACCAGGAAGCGAGCTCCAGCGGCGCGACCTCGGTTTCCTTCAAGGAGGCCGCACTGTCTCTGGAAGTCACTCC
GCAGATCACCCCTGACAACCGCATCATCATGCAGGTCAAGGTGAACAAGGACGAGCCGGACTACCAGAACGCACTGCTCG
GCGTTCCGCCGATCAAGAAGAACGAGGTCAATGCCAAGGTCCTGGTCAATGACGGCGAGACCATCGTTATCGGTGGTGTG
TTCTCCAACACTCAGACCAAGACGACCGCGAAGGTACCGTTCCTGGGTGATGTGCCGGTAGTCGGTCGCCTGTTCAAGAA
TGACTACGTGACGGACACCAAGAACGAGCTTCTGGTTTTCCTCACTCCCCGGATCATGAATAATCAGGCCATTGCTATCG
ATCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(530-686)

AMIN domain (AMIN)

(40-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(380-454)

Secretin and TonB N terminus short domain (STN)

(306-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

76.105

100

0.793
  comQ Acinetobacter baylyi ADP1

42.341

99.568

0.422
  pilQ Acinetobacter baumannii strain A118

40.48

100

0.413
  pilQ Acinetobacter baumannii D1279779

40.48

100

0.413
  pilQ Legionella pneumophila strain Lp02

38.563

98.129

0.378
  pilQ Legionella pneumophila strain ERS1305867

38.563

98.129

0.378

Multiple sequence alignment