Detailed information NTG1111939

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: PKB_RS26370
• Genome accession: NZ_HG322950
• Coordinates: 5643028..5644092 (-)
• Length: 354 a.a.
• NCBI ID: WP_043255941.1
• Uniprot ID: A0A024HQD8
• Organism: Pseudomonas knackmussii B13
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5638028..5649092

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PKB_RS26345 (PKB_5346)	aroK	5638559..5639077 (-)	519	WP_043257824.1	shikimate kinase AroK	-
PKB_RS26350 (PKB_5347)	pilQ	5639086..5641173 (-)	2088	WP_043255934.1	type IV pilus secretin PilQ	Machinery gene
PKB_RS26355 (PKB_5348)	pilP	5641231..5641755 (-)	525	WP_043255936.1	type 4a pilus biogenesis lipoprotein PilP	-
PKB_RS26360 (PKB_5349)	pilO	5641752..5642432 (-)	681	WP_052355394.1	type 4a pilus biogenesis protein PilO	-
PKB_RS26365 (PKB_5350)	pilN	5642429..5643028 (-)	600	WP_043255938.1	type 4a pilus biogenesis protein PilN	-
PKB_RS26370 (PKB_5351)	pilM	5643028..5644092 (-)	1065	WP_043255941.1	pilus assembly protein PilM	Machinery gene
PKB_RS26375 (PKB_5352)	-	5644278..5646719 (+)	2442	WP_043255943.1	penicillin-binding protein 1A	-
PKB_RS26380 (PKB_5353)	-	5646911..5647402 (-)	492	WP_043257828.1	M48 family metallopeptidase	-
PKB_RS26385 (PKB_5354)	-	5647466..5648236 (-)	771	WP_043255945.1	class I SAM-dependent methyltransferase	-
PKB_RS26390 (PKB_5355)	-	5648278..5648703 (-)	426	WP_043255947.1	hypothetical protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37754.09 Da
• Isoelectric Point: 4.4567

>NTDB_id=1111939 PKB_RS26370 WP_043255941.1 5643028..5644092(-) (pilM) [Pseudomonas knackmussii B13]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGGRFKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVVAKARTGVKTAAV
AVAGSAVITKSIEMDAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGVSARNAERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAFGLLGDQLGGNADELTIAVVDIGATMTTLSVLHHGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYESEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTPTLVANP
FADMALSNKVNAGALASDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1111939 PKB_RS26370 WP_043255941.1 5643028..5644092(-) (pilM) [Pseudomonas knackmussii B13]
GTGCTAGGGCTCTTCAATAAGAAAGCGAACACACTGCTGGGGATCGACATCAGTTCGACTTCGGTCAAGCTCCTTGAGTT
GAGCCGCTCCGGTGGCCGCTTCAAGGTCGAGGCTTACGCGGTCGAGCCGCTGCCCCCGAACGCGGTGGTAGAAAAGAACA
TCGCCGAACTGGAGGGGGTCGGCCAGGCGCTGTCGCGCGTGGTCGCCAAGGCGCGCACCGGCGTGAAAACCGCCGCCGTC
GCCGTCGCCGGATCGGCGGTCATCACCAAATCCATCGAGATGGATGCTGGCCTCTCCGAGGACGAGCTGGAAAACCAGCT
CAAGATCGAGGCGGACCAGTACATCCCCTACCCCCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGGTATCGGCTC
GCAACGCTGAGCGCGTCGATGTGCTCCTGGCGGCCTGCCGCAAGGAAAACGTCGAGGTGCGCGAAGCTGCGCTGGCGCTT
GCAGGGCTGACCGCCAAGGTGGTCGATGTCGAGGCCTACGCCCTGGAGCGCGCCTTCGGTCTGCTCGGTGACCAGTTGGG
CGGTAACGCCGATGAGCTGACGATCGCTGTGGTCGACATTGGCGCGACCATGACGACCCTGAGCGTGCTGCACCACGGCC
GCACTATCTATACCCGTGAGCAACTGTTCGGCGGGCGCCAACTTACCGAAGAGATCCAGCGTCGCTATGGTCTGTCGGTC
GAAGAGGCTGGCCTGGCGAAGAAACAGGGCGGGCTCCCGGACGACTACGAAAGCGAAGTGCTGCAGCCGTTCAAGGATGC
GGTGGTTCAGCAGGTCTCCCGTTCCCTGCAGTTCTTCTTCGCCGCAGGTCAGTTCAACGACGTCGATTACATCCTGCTCG
CCGGTGGCACGGCTTCGATCCCAGATCTGGATCGCCTGATCCAGCAGAAGATCGGCACGCCCACCCTGGTAGCCAACCCC
TTCGCCGACATGGCCCTGAGCAACAAGGTGAATGCCGGGGCGCTGGCCAGTGATGCGCCCGCGCTGATGATTGCCTGCGG
TCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A024HQD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	57.345	100	0.573
comM	Acinetobacter nosocomialis M2	57.345	100	0.573
comM	Acinetobacter baylyi ADP1	52.825	100	0.528
pilM	Legionella pneumophila strain ERS1305867	46.328	100	0.463