Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GJA_RS20645 Genome accession   NZ_HG322949
Coordinates   4818130..4819857 (+) Length   575 a.a.
NCBI ID   WP_038496049.1    Uniprot ID   -
Organism   Janthinobacterium agaricidamnosum NBRC 102515 = DSM 9628     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4813130..4824857
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJA_RS20635 (GJA_4448) - 4814972..4816447 (+) 1476 WP_081905661.1 DHA2 family efflux MFS transporter permease subunit -
  GJA_RS20640 (GJA_4449) - 4816643..4817941 (+) 1299 WP_038496047.1 HlyC/CorC family transporter -
  GJA_RS20645 (GJA_4450) pilF 4818130..4819857 (+) 1728 WP_038496049.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GJA_RS20650 (GJA_4451) pilC 4819891..4821120 (+) 1230 WP_038496051.1 type II secretion system F family protein Machinery gene
  GJA_RS20655 (GJA_4452) - 4821525..4821725 (+) 201 WP_144241606.1 hypothetical protein -
  GJA_RS20660 - 4821722..4821991 (-) 270 WP_038496054.1 hypothetical protein -
  GJA_RS20665 (GJA_4453) pilD 4822300..4823169 (+) 870 WP_038496056.1 A24 family peptidase Machinery gene
  GJA_RS20670 (GJA_4454) coaE 4823209..4823856 (+) 648 WP_038496058.1 dephospho-CoA kinase -
  GJA_RS20675 (GJA_4455) zapD 4823921..4824676 (+) 756 WP_038496060.1 cell division protein ZapD -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62542.17 Da        Isoelectric Point: 5.6948

>NTDB_id=1111903 GJA_RS20645 WP_038496049.1 4818130..4819857(+) (pilF) [Janthinobacterium agaricidamnosum NBRC 102515 = DSM 9628]
MAAVSPIVAQASGTAMPGLARALLQAGHLNAAVADTLHKKSLQEKQSFIDVLLSSGAIDAAGLALFCSETFAYPLLDLRT
LSLDALPQKSIDNRLMQSQRVIPLTRRGNKMSVAISDPTNTQALDQIKFQTEASVEPVIVQHDVLLQVLASLNKSSEQII
SDLGGDHQEIQFAEEETAAPDPSANEVEDAPIVRFLNKILMDAVNLGASDIHFEPFEKYFRIRLRVDGVLLDHAQPPVSI
REKLVSRIKVLARLDISEKRIPQDGRMRLILSPVRTIDFRVSTLPTLFGEKTVMRILDATQAQMGIDALGYEPDQKALLL
DAITRPYGMVLVTGPTGSGKTVSLYTCLNVINKPGINISTAEDPAEINMPGVNQVNVNERAGLTFPVALKSFLRQDPDVI
MVGEIRDLETADIAIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSVILITAQRLTRRLCSCKQPVEMIDELL
LKAGFLPEELDGSWKPHGPVGCERCNGTGYKGRVGIYQIMPITEAIEKIILAHGNSLEIARQAESEGVVSLRRAGLSKVR
QGLTSLEEVLGCTNE

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=1111903 GJA_RS20645 WP_038496049.1 4818130..4819857(+) (pilF) [Janthinobacterium agaricidamnosum NBRC 102515 = DSM 9628]
ATGGCAGCAGTCTCTCCTATCGTCGCTCAAGCTTCCGGCACGGCCATGCCTGGCCTGGCGCGAGCATTGCTGCAAGCCGG
GCATTTGAATGCCGCGGTGGCCGATACGCTGCACAAAAAATCATTACAAGAAAAGCAATCCTTCATCGATGTGCTGCTGT
CCAGCGGCGCCATCGACGCGGCCGGGCTGGCGCTGTTTTGCTCGGAAACCTTTGCCTACCCGCTACTCGATTTACGCACC
CTGAGCCTGGATGCGCTGCCGCAAAAAAGCATCGACAACCGCTTGATGCAAAGCCAGCGCGTGATTCCGCTGACACGGCG
CGGCAACAAGATGTCGGTGGCGATTTCCGATCCGACCAATACCCAGGCGCTGGACCAGATCAAGTTCCAGACCGAAGCGT
CGGTGGAACCGGTGATCGTGCAGCATGACGTGCTGCTGCAAGTGCTGGCCAGCCTGAATAAAAGCAGCGAGCAAATCATC
AGCGACCTGGGCGGCGATCACCAGGAAATCCAGTTCGCCGAAGAAGAAACCGCCGCGCCCGATCCTTCCGCCAATGAAGT
CGAAGATGCGCCCATCGTGCGCTTCCTCAACAAGATCTTGATGGATGCGGTCAACCTGGGCGCGTCGGATATCCATTTTG
AACCGTTCGAGAAATATTTCCGGATACGCTTGCGGGTCGATGGCGTGTTGCTCGATCACGCGCAGCCGCCGGTATCGATC
CGGGAAAAACTCGTGTCGCGCATCAAGGTGCTGGCGCGGCTGGATATTTCCGAAAAGCGCATCCCGCAAGACGGCCGCAT
GCGGCTGATCCTGTCGCCGGTGCGCACCATCGATTTCCGGGTCAGCACGCTGCCGACGCTGTTCGGCGAAAAAACCGTGA
TGCGGATACTCGACGCCACCCAGGCCCAGATGGGCATCGACGCGCTCGGTTACGAGCCGGACCAGAAAGCGCTGCTGCTC
GATGCAATTACCCGTCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCCGGCAAGACCGTGTCGCTGTACACTTG
CCTGAACGTGATCAACAAGCCCGGCATCAATATTTCAACGGCGGAAGATCCAGCCGAGATCAATATGCCGGGCGTCAACC
AGGTCAACGTCAATGAACGGGCCGGCCTGACCTTCCCGGTGGCGTTGAAATCGTTCCTGCGGCAAGATCCGGACGTCATC
ATGGTCGGCGAGATCCGCGACCTGGAAACCGCCGACATCGCCATCAAGGCGGCGCAAACCGGCCACATGGTGTTTTCCAC
CTTGCATACCAACGATGCCCCGTCGACGCTGACGCGCTTGATGAATATGGGCGTGGCGCCGTTCAATATCGCCTCGTCGG
TGATCCTGATCACGGCGCAGCGCCTGACCCGCCGCCTGTGTTCCTGCAAGCAGCCGGTCGAGATGATCGATGAACTGCTG
CTGAAAGCCGGTTTCCTGCCGGAAGAACTCGATGGCAGCTGGAAACCGCATGGCCCGGTCGGCTGCGAACGCTGCAACGG
CACCGGCTACAAGGGCCGGGTCGGGATTTACCAGATCATGCCGATCACCGAAGCGATCGAGAAAATCATCCTGGCGCACG
GCAACTCGCTGGAAATTGCCCGCCAGGCCGAATCGGAAGGCGTGGTGTCGCTGCGCCGGGCCGGCTTGAGCAAGGTGCGG
CAAGGATTGACCAGCCTCGAAGAAGTGTTAGGCTGTACTAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-465)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-156)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

54.367

97.565

0.53
  pilB Acinetobacter baylyi ADP1

53.004

98.435

0.522
  pilB Acinetobacter baumannii D1279779

52.734

98.609

0.52
  pilB Legionella pneumophila strain ERS1305867

47.059

97.565

0.459
  pilB Vibrio parahaemolyticus RIMD 2210633

45.536

97.391

0.443
  pilB Vibrio campbellii strain DS40M4

44.248

98.261

0.435
  pilB Vibrio cholerae strain A1552

46.981

92.174

0.433
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.962

91.826

0.367
  pilF Thermus thermophilus HB27

39.286

92.522

0.363

Multiple sequence alignment