Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACOI2Q_RS20135 Genome accession   NZ_CP184785
Coordinates   4498039..4499745 (+) Length   568 a.a.
NCBI ID   WP_420789190.1    Uniprot ID   -
Organism   Shewanella algae strain NJT6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4493039..4504745
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOI2Q_RS20110 (ACOI2Q_20110) pdhR 4493195..4493947 (-) 753 WP_025886793.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  ACOI2Q_RS20115 (ACOI2Q_20115) ampE 4494222..4495073 (-) 852 WP_025012050.1 beta-lactamase regulator AmpE -
  ACOI2Q_RS20120 (ACOI2Q_20120) ampD 4495127..4495705 (-) 579 WP_051547208.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACOI2Q_RS20125 (ACOI2Q_20125) nadC 4495864..4496724 (+) 861 WP_025012052.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACOI2Q_RS20130 (ACOI2Q_20130) - 4497593..4498024 (+) 432 WP_342197588.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACOI2Q_RS20135 (ACOI2Q_20135) pilB 4498039..4499745 (+) 1707 WP_420789190.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACOI2Q_RS20140 (ACOI2Q_20140) pilC 4499907..4501172 (+) 1266 WP_420789192.1 type II secretion system F family protein Machinery gene
  ACOI2Q_RS20145 (ACOI2Q_20145) pilD 4501242..4502156 (+) 915 WP_342197585.1 A24 family peptidase Machinery gene
  ACOI2Q_RS20150 (ACOI2Q_20150) coaE 4502157..4502768 (+) 612 WP_054744929.1 dephospho-CoA kinase -
  ACOI2Q_RS20155 (ACOI2Q_20155) zapD 4502765..4503505 (+) 741 WP_028779562.1 cell division protein ZapD -
  ACOI2Q_RS20160 (ACOI2Q_20160) yacG 4503550..4503762 (+) 213 WP_025886789.1 DNA gyrase inhibitor YacG -
  ACOI2Q_RS20165 (ACOI2Q_20165) mutT 4503762..4504160 (+) 399 WP_420789195.1 8-oxo-dGTP diphosphatase MutT -
  ACOI2Q_RS20170 (ACOI2Q_20170) - 4504231..4504500 (+) 270 Protein_3924 transposase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63117.32 Da        Isoelectric Point: 5.7375

>NTDB_id=1111265 ACOI2Q_RS20135 WP_420789190.1 4498039..4499745(+) (pilB) [Shewanella algae strain NJT6]
MPTSGLHLGLSTLFIRKGLLTESQLTETIRHSQNSKQTLVTTLVTSKLLSARTIAELCYEEYGTPLLDLNEFDLSSIPEE
FLNKKLIDKHRCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEEDKLSKALEKVLEEDLSSLDLNGLDEES
LAGIEVADTDKRQQEENEGKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLSGRISAR
LKVMSKLDIAERRVPQDGRIKMKLSRNKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEEDQKQAYLEMLARPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGINQVHINTKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSSAETLTRLLNMGVPGYNIASSVNLIIAQRLARRLCPECRQPEDIPKHELLQLGFT
QQQIDAGFTPYKPCGCEFCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIATTAKQQGMRDLRLSGLLKVIQGVTSIA
EVNRVTSY

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=1111265 ACOI2Q_RS20135 WP_420789190.1 4498039..4499745(+) (pilB) [Shewanella algae strain NJT6]
ATGCCAACATCCGGCTTACATCTAGGTCTGTCGACTCTTTTTATCCGGAAGGGGCTACTGACGGAAAGTCAGTTAACCGA
AACGATAAGACATTCCCAGAATAGTAAACAAACACTGGTCACCACTCTGGTGACCAGTAAGCTTTTATCTGCTCGTACCA
TCGCCGAACTCTGTTACGAAGAGTATGGCACCCCGCTGCTGGATCTCAATGAATTCGACCTAAGCAGCATCCCGGAAGAG
TTTCTCAATAAAAAGCTGATCGATAAGCACAGATGTCTGCCGCTATTTAAACGTGGCAACCGCCTCTATATTGCCACCTC
AGACCCCACCAATATAGCCGCCCTGGAAGATTTTCAATTTAGTGCCGGCCTGCACGCCGAAGCGATACTGGTAGAGGAGG
ATAAGCTTAGTAAAGCGCTGGAAAAAGTGCTTGAAGAGGACTTGAGTTCATTGGATCTTAATGGTCTCGATGAGGAATCC
CTCGCAGGAATTGAAGTCGCCGATACCGACAAGCGTCAGCAGGAAGAGAATGAAGGCAAAGACGATGCTCCTATCGTCAT
CTATATCAATAAGATTTTGACCGATGCCATCCGCAAGGGCGCATCGGATCTGCATTTCGAGCCCTACGAGAAACGCTACC
GCATCCGTTTTCGTATTGATGGCATCCTCCATGAGGTGTCCGAGCCACCAATTTCTCTCTCGGGCCGTATCTCAGCCCGC
TTGAAAGTGATGTCCAAATTGGATATCGCCGAGCGCCGGGTACCTCAGGATGGCCGCATCAAGATGAAGCTGTCCCGCAA
CAAGAGTATCGATTTCCGGGTAAGTACCCTGCCCACCCTCTGGGGTGAAAAGATAGTTATGCGGATCCTCGACTCCTCTT
CCGCGCAATTAGGCATCGAAAAACTCGGCTATGAGGAAGACCAAAAACAAGCCTATCTGGAGATGCTGGCAAGGCCCCAG
GGGATGATCTTGGTAACAGGCCCTACCGGCTCTGGTAAAACCGTGTCCCTCTACACTGGCCTCAATATCCTCAATACCGA
AGAGCGCAATATCAGCACCGCTGAAGACCCGGTGGAGATCAACCTGGAAGGGATCAACCAGGTACATATCAACACCAAGG
CCGGGTTAACCTTCGCTTCGGCGCTGCGCTCTTTTTTACGTCAGGACCCGGATGTGGTGATGGTGGGGGAAATTCGCGAT
TTGGAAACCGCCGAAATCGCCATTAAGGCGGCGCAAACCGGCCACTTGGTACTCTCAACCCTGCACACCAATTCATCGGC
CGAAACCCTCACCCGCCTGCTTAATATGGGTGTCCCAGGTTATAACATTGCCAGTTCGGTCAACCTGATTATTGCCCAGC
GCCTGGCCCGCCGCCTCTGCCCCGAATGCCGCCAACCGGAAGATATCCCCAAGCATGAACTGCTGCAACTGGGCTTCACT
CAGCAGCAGATAGACGCAGGCTTTACCCCATACAAGCCCTGTGGCTGCGAATTCTGCTCCGGTGGCTATAAGGGCCGGGT
CGGTATCTATGAGGTAATGAAGATGTCCGATGAAATCGCCAGAACCATTATGGAGGGCGGTAATTCACTGCAGATTGCCA
CCACCGCCAAACAACAAGGGATGCGCGACCTGCGCCTTTCAGGCCTTTTAAAAGTGATTCAAGGCGTCACCAGTATCGCC
GAAGTGAACCGGGTCACCAGCTACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-145)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.554

99.824

0.585
  pilB Acinetobacter baylyi ADP1

58.467

98.768

0.577
  pilB Legionella pneumophila strain ERS1305867

56.36

99.648

0.562
  pilB Vibrio parahaemolyticus RIMD 2210633

53.274

99.472

0.53
  pilB Vibrio campbellii strain DS40M4

51.865

99.12

0.514
  pilB Vibrio cholerae strain A1552

52.717

97.183

0.512
  pilF Neisseria gonorrhoeae MS11

48.142

99.472

0.479
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.962

93.838

0.375
  pilF Thermus thermophilus HB27

37.168

99.472

0.37

Multiple sequence alignment