Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACN079_RS07470 Genome accession   NZ_CP184478
Coordinates   1659107..1661194 (+) Length   695 a.a.
NCBI ID   WP_420234178.1    Uniprot ID   -
Organism   Pseudomonas sp. ABY48     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1654107..1666194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN079_RS07450 (ACN079_07450) comM 1656318..1657382 (+) 1065 WP_420234174.1 pilus assembly protein PilM Machinery gene
  ACN079_RS07455 (ACN079_07455) - 1657382..1657948 (+) 567 WP_420234175.1 PilN domain-containing protein -
  ACN079_RS07460 (ACN079_07460) pilO 1657945..1658568 (+) 624 WP_420234176.1 type 4a pilus biogenesis protein PilO -
  ACN079_RS07465 (ACN079_07465) - 1658565..1659110 (+) 546 WP_420234177.1 pilus assembly protein PilP -
  ACN079_RS07470 (ACN079_07470) pilQ 1659107..1661194 (+) 2088 WP_420234178.1 type IV pilus secretin PilQ Machinery gene
  ACN079_RS07475 (ACN079_07475) aroK 1661199..1661717 (+) 519 WP_003196983.1 shikimate kinase AroK -
  ACN079_RS07480 (ACN079_07480) aroB 1661866..1662966 (+) 1101 WP_420234179.1 3-dehydroquinate synthase -
  ACN079_RS07485 (ACN079_07485) - 1662977..1664530 (+) 1554 WP_420234180.1 AAA family ATPase -

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 74927.98 Da        Isoelectric Point: 5.2895

>NTDB_id=1108412 ACN079_RS07470 WP_420234178.1 1659107..1661194(+) (pilQ) [Pseudomonas sp. ABY48]
MNRIFSALGISLWIALLSPMVHGASLKTLDVAALPGDRVELKLTFDGPPPPPRGYTTEQPARIALDLPGVTNQLSSKRRD
LGGGNARSATVVEAGDRTRLIIGLTQLAPYDTRVEGNNLFVVVGKGAASNSTVPKSAAQAPRPAVASAAPARASAPVGKA
IRGVDFQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILNFAKTQLPEPLRVRLDVKDFATPVQFVNASVGSDRATISIEP
SGTFDYSTYQTDNKLTVSVRPMSVDDLQKRNADRAAYSGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRL
QNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSA
EAKADERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWSSSG
VTTGDSTTIGTPGSTSTNQPFVDLGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTE
IPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVNDVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSDSKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=1108412 ACN079_RS07470 WP_420234178.1 1659107..1661194(+) (pilQ) [Pseudomonas sp. ABY48]
ATGAACAGGATTTTTTCAGCCCTCGGTATTTCGCTATGGATAGCGCTGTTGTCGCCGATGGTTCATGGGGCCAGCCTGAA
GACGCTGGATGTGGCGGCGTTGCCGGGGGACCGTGTCGAGTTGAAGCTCACCTTCGACGGGCCACCGCCGCCACCCCGGG
GCTACACCACCGAACAACCGGCGCGGATTGCCCTGGATCTGCCGGGTGTGACCAATCAACTTTCCAGCAAGCGCCGCGAC
CTGGGCGGGGGCAATGCACGCAGCGCCACCGTGGTCGAGGCTGGGGATCGCACCCGGTTGATCATCGGCCTTACGCAACT
GGCGCCGTATGACACCCGGGTCGAAGGTAACAACCTGTTCGTGGTGGTTGGCAAGGGCGCCGCCTCGAACAGCACTGTCC
CGAAGAGCGCCGCCCAGGCCCCGCGCCCGGCGGTTGCCAGCGCTGCGCCTGCGAGGGCCAGTGCGCCGGTGGGCAAGGCG
ATCCGTGGGGTGGACTTCCAGCGCGGGACCCAGGGCGAAGGCAACGTAGTGATCGACCTGTCTGATCCGTCCATCGCCCC
GGACATTCAGGAGCGCGAAGGCAAGATCATCCTCAATTTCGCCAAGACCCAGTTGCCCGAGCCACTGCGGGTGCGCCTGG
ACGTCAAGGACTTCGCCACCCCGGTGCAGTTCGTCAATGCCAGCGTCGGCAGTGACCGGGCGACCATCAGCATCGAACCC
AGCGGCACCTTCGACTACTCGACCTACCAGACCGACAACAAACTCACCGTCAGCGTCCGGCCCATGAGCGTCGACGACCT
GCAGAAACGCAACGCCGACCGCGCGGCCTACAGTGGCGAGAAGCTCTCGCTCAACTTCCAGGACATCGAGGTGCGTTCGG
TCCTGCAACTGATTGCTGATTTTACCAACCTGAACCTCGTGGCCAGCGACACGGTGCAGGGCGGCATCACCTTGCGGTTG
CAGAATGTGCCCTGGGACCAGGCGCTCGACCTGGTGCTCAAGACCAAGGGGTTGGACAAGCGCAAGGTTGGCAACGTGCT
GTTGGTGGCGCCGGCCGATGAAATTGCCGCCCGTGAGCGTCAGGAACTGGAGTCGCAGAAGCAGATCGCCGACCTGGCAC
CGCTGCGGCGTGAGCTGTTGCAGGTCAACTATGCGAAGGCGGCGGACATCGCCAAGCTGTTCCAATCGGTGACCAGTGCC
GAGGCGAAAGCCGATGAGCGCGGCACGATCACGGTCGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGACCG
CCTCGATGAACTGCGCCGGATCGTGGCGCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGGCGCGGATTGTCGAGG
CTAACGTCGACTACGACAAAAGCCTGGGCGTGCGCTGGGGCGGTTCGATTCAAAACAAGGGCAACTGGAGCTCCTCGGGG
GTCACCACGGGCGACTCCACCACCATTGGCACGCCGGGCAGCACCAGCACTAACCAGCCGTTCGTCGACCTGGGCGCCTC
GGGCAACACCTCGGGCATCGGCATTGCCTTCATCACCGACAACGTATTGCTGGACCTTGAGCTGACGGCCATGGAAAAAA
CCGGCAACGGGGAGATCGTCTCCCAGCCCAAGGTGGTGACGTCCGACAAGGAAACCGCAAAGATCCTCAAGGGCACGGAA
ATTCCCTATCAGGAAGCCAGCTCCAGCGGCGCTACGTCGGTGTCGTTCAAGGAGGCTTCGCTGTCGCTGGAGGTGACCCC
GCAGATCACTCCAGACAACCGGATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAGGTGAATG
ATGTGCCGCCTATCAAGAAAAACGAGGTCAATGCCAAGGTCCTGGTCAATGATGGCGAGACCATTGTGATTGGCGGGGTT
TTCTCAAATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCG
TGATGTGGTTTCGGATAGCAAATCCGAGCTGCTGGTGTTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGA
GTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(530-686)

Bacterial type II/III secretion system short domain (Secretin_N)

(379-444)

Secretin and TonB N terminus short domain (STN)

(305-351)

AMIN domain (AMIN)

(38-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.464

100

0.757
  pilQ Acinetobacter baumannii strain A118

38.77

100

0.417
  pilQ Acinetobacter baumannii D1279779

38.658

100

0.414
  comQ Acinetobacter baylyi ADP1

41.007

100

0.41
  pilQ Legionella pneumophila strain Lp02

38.529

97.842

0.377
  pilQ Legionella pneumophila strain ERS1305867

37.997

97.698

0.371

Multiple sequence alignment