Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACGJAX_RS07945 Genome accession   NZ_AP035776
Coordinates   1683060..1685132 (+) Length   690 a.a.
NCBI ID   WP_390958257.1    Uniprot ID   -
Organism   Pseudomonas moorei strain m318     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1678060..1690132
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGJAX_RS07925 (PMm318_A15580) pilM 1680275..1681336 (+) 1062 WP_390958249.1 type IV pilus assembly protein PilM Machinery gene
  ACGJAX_RS07930 (PMm318_A15590) - 1681336..1681902 (+) 567 WP_390958251.1 PilN domain-containing protein -
  ACGJAX_RS07935 (PMm318_A15600) - 1681899..1682522 (+) 624 WP_390958253.1 type 4a pilus biogenesis protein PilO -
  ACGJAX_RS07940 (PMm318_A15610) - 1682519..1683046 (+) 528 WP_390958255.1 pilus assembly protein PilP -
  ACGJAX_RS07945 (PMm318_A15620) pilQ 1683060..1685132 (+) 2073 WP_390958257.1 type IV pilus secretin PilQ Machinery gene
  ACGJAX_RS07950 (PMm318_A15630) aroK 1685137..1685655 (+) 519 WP_090328238.1 shikimate kinase AroK -
  ACGJAX_RS07955 (PMm318_A15640) aroB 1685808..1686908 (+) 1101 WP_090328241.1 3-dehydroquinate synthase -
  ACGJAX_RS07960 (PMm318_A15650) - 1686919..1688523 (+) 1605 WP_390958259.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74411.53 Da        Isoelectric Point: 5.2808

>NTDB_id=110780 ACGJAX_RS07945 WP_390958257.1 1683060..1685132(+) (pilQ) [Pseudomonas moorei strain m318]
MNRTFSAIGMTLWMALLSPMAQAASLKALDVVALPGDRVELKLAFDEPPPAPQGYTTASPARIALDLPGVVSQLANKTRD
LGAGNARSATVVEVKDRTRLIINLSQSAPYSTRIEGNNLLVVVGQGGKNATARSTAIAAPAPARPPVPSGKAIGGVDFQR
GTQGEGNVVIDLSDPSIAPDIQERDGKIIVGFARTHLPEPLRVRLDVKDFATPVQFINASAIGDRAIITIEPGGGAFDYS
TYQTDNKLTVSVRPVTTDDLQKRNADRYTYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSAEAKVDER
GSITVDERTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSNGANG
SSTIGTPGSTSTNSPFVDLGAANNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
ASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=110780 ACGJAX_RS07945 WP_390958257.1 1683060..1685132(+) (pilQ) [Pseudomonas moorei strain m318]
ATGAACAGGACTTTTTCAGCCATCGGAATGACGCTATGGATGGCGTTGTTGTCGCCGATGGCACAAGCGGCCAGCCTCAA
GGCACTGGATGTCGTGGCGCTGCCGGGAGATCGCGTCGAGTTGAAGCTGGCATTCGATGAGCCGCCACCGGCCCCTCAGG
GCTACACCACAGCGTCACCGGCGCGGATCGCGCTGGATTTGCCGGGAGTCGTCAGTCAATTGGCGAACAAAACCCGTGAC
CTGGGTGCTGGCAATGCCCGAAGCGCGACGGTGGTGGAGGTCAAGGATCGTACGCGGCTGATCATCAATTTGAGCCAATC
GGCCCCCTACAGCACGCGAATCGAGGGCAACAACCTGTTGGTGGTGGTCGGGCAGGGCGGCAAAAACGCGACGGCCAGAT
CGACCGCCATTGCAGCGCCGGCTCCGGCGCGCCCCCCAGTGCCATCGGGCAAGGCTATCGGCGGTGTGGACTTTCAGCGT
GGTACCCAGGGTGAAGGCAATGTGGTGATCGATCTGTCTGACCCGTCCATTGCGCCGGATATTCAGGAGCGCGACGGCAA
GATCATCGTCGGGTTTGCCAGGACTCACTTGCCCGAACCCTTGCGCGTGCGTCTGGATGTGAAGGACTTCGCCACGCCGG
TGCAGTTCATCAACGCCAGCGCCATCGGTGACCGGGCCATCATCACGATCGAGCCTGGCGGTGGCGCCTTCGACTACTCG
ACCTATCAGACCGACAACAAACTCACGGTCAGCGTCCGTCCGGTGACCACCGACGACCTGCAAAAGCGTAACGCCGACCG
CTACACCTACAGTGGTGAAAAGCTTTCGCTGAACTTCCAGGACATCGACGTGCGCTCAGTGCTGCAACTGATCGCGGACT
TCACCAATCTCAATCTGGTCGCCAGCGATACGGTACAGGGCGGCATTACCCTGCGCCTGCAGAACGTCCCGTGGGATCAG
GCACTGGATCTGGTGCTGAAAACCAAGGGGCTGGACAAGCGCAAGATCGGTAACGTGCTACTGGTGGCGCCGGCCGACGA
AATTGCCGCCCGTGAGCGTCAGGAGCTGGAGTCGCAAAAACAGATCGCCGATCTGGCACCGCTGCGGCGGGAGCTTTTGC
AGGTCAATTACGCCAAGGCGGCGGATATCGCCAAACTGTTCCAGTCGGTGACCAGTGCCGAGGCGAAAGTCGACGAGCGG
GGCTCCATCACTGTTGATGAGCGTACCAACAACATCATTGCCTACCAGACTCAGGAGCGACTCGACGAGCTGCGACGCAT
CGTTGCCCAGCTGGATATTCCGGTGCGCCAGGTCATGATCGAAGCACGCATCGTCGAAGCCAACGTCGATTACGACAAAA
GCCTGGGTGTGCGCTGGGGCGGTTCGATCCAGAACAAGGGCAACTGGAATACATCCGGGGTCAGCAACGGCGCCAACGGA
TCATCGACCATCGGTACACCGGGTAGCACCAGCACCAATTCACCGTTCGTCGACTTGGGGGCGGCGAACAACACCTCAGG
GATCGGCATTGCGTTCATTACCGATAACGTCTTGCTGGACCTGGAACTGACGGCCATGGAGAAGACCGGCAACGGGGAAA
TCGTCTCGCAACCCAAGGTCGTCACGTCCGACAAGGAAACCGCAAAAATCCTCAAGGGCACCGAGATTCCCTATCAGGAA
GCCAGCTCCAGCGGCGCTACATCGGTGTCTTTCAAAGAGGCATCGCTCTCGCTGGAAGTGACGCCGCAGATCACTCCGGA
CAACCGGATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTCAACAAGGTCCAGGATGTGCCGCCCATCA
AGAAAAACGAGGTCAACGCCAAGGTCCTGGTCAACGATGGGGAGACCATCGTCATTGGTGGTGTTTTCTCAAATACTCAA
AGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGACGTGGTTTCGGA
GAAAAAATCCGAGCTGTTGGTGTTTCTCACACCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Secretin and TonB N terminus short domain (STN)

(298-344)

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

AMIN domain (AMIN)

(37-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.486

100

0.752
  comQ Acinetobacter baylyi ADP1

41.103

100

0.432
  pilQ Acinetobacter baumannii strain A118

38.211

100

0.409
  pilQ Acinetobacter baumannii D1279779

38.211

100

0.409
  pilQ Legionella pneumophila strain ERS1305867

39.466

97.681

0.386
  pilQ Legionella pneumophila strain Lp02

39.227

97.536

0.383

Multiple sequence alignment