Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACGJAX_RS07925 Genome accession   NZ_AP035776
Coordinates   1680275..1681336 (+) Length   353 a.a.
NCBI ID   WP_390958249.1    Uniprot ID   -
Organism   Pseudomonas moorei strain m318     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1675275..1686336
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGJAX_RS07915 (PMm318_A15560) - 1676144..1677412 (+) 1269 WP_090328218.1 malic enzyme-like NAD(P)-binding protein -
  ACGJAX_RS07920 (PMm318_A15570) - 1677622..1680060 (-) 2439 WP_390959818.1 PBP1A family penicillin-binding protein -
  ACGJAX_RS07925 (PMm318_A15580) pilM 1680275..1681336 (+) 1062 WP_390958249.1 type IV pilus assembly protein PilM Machinery gene
  ACGJAX_RS07930 (PMm318_A15590) - 1681336..1681902 (+) 567 WP_390958251.1 PilN domain-containing protein -
  ACGJAX_RS07935 (PMm318_A15600) - 1681899..1682522 (+) 624 WP_390958253.1 type 4a pilus biogenesis protein PilO -
  ACGJAX_RS07940 (PMm318_A15610) - 1682519..1683046 (+) 528 WP_390958255.1 pilus assembly protein PilP -
  ACGJAX_RS07945 (PMm318_A15620) pilQ 1683060..1685132 (+) 2073 WP_390958257.1 type IV pilus secretin PilQ Machinery gene
  ACGJAX_RS07950 (PMm318_A15630) aroK 1685137..1685655 (+) 519 WP_090328238.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 38099.87 Da        Isoelectric Point: 5.0005

>NTDB_id=110779 ACGJAX_RS07925 WP_390958249.1 1680275..1681336(+) (pilM) [Pseudomonas moorei strain m318]
MPGLFNKKPDTLLGIDISSTSVKLLALSRQGARYRVEAYAVEPLPANAVIEKNIAELEAVGQALSRVLIKARTSLKHVAV
AVAGSAVITKIIEMDAGLCDDDMENQLKIEADQYIPYPLDEVAIDFEVQGVSSRNPERVDVLLAACRKESVEVREAALAL
AGLTARVVDVEAHALERSFGLLANQSVVPKERLVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTEDIQRRYGLTLE
QAGLAKKQGGLPDDYVNEVLQPFREALVQQVARSLQFFFAGGQYDAVDHILLVGGTASICGLDRLIQEHLGTPTQVANPF
VEMTLSAKVNAAALASDAPAMMIACGLALRSFD

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=110779 ACGJAX_RS07925 WP_390958249.1 1680275..1681336(+) (pilM) [Pseudomonas moorei strain m318]
GTGCCAGGACTCTTCAACAAAAAGCCCGACACCCTTTTGGGTATCGACATCAGCTCCACTTCGGTGAAGCTTCTGGCGCT
GAGCCGCCAGGGCGCCCGTTATCGGGTCGAGGCCTATGCGGTCGAACCGCTGCCCGCCAACGCCGTTATCGAAAAAAATA
TTGCCGAGCTCGAAGCTGTGGGCCAGGCGCTCTCTCGCGTATTGATCAAGGCCAGAACCAGCCTCAAGCACGTGGCTGTG
GCGGTGGCTGGCTCGGCGGTGATCACCAAGATCATCGAGATGGATGCCGGACTTTGTGACGACGACATGGAAAACCAGCT
GAAGATCGAGGCCGATCAGTACATCCCCTATCCGCTGGATGAAGTCGCCATCGATTTTGAAGTCCAAGGGGTTTCGTCGC
GCAATCCAGAGCGGGTCGATGTGCTGCTCGCGGCCTGTCGCAAGGAAAGCGTCGAAGTCCGTGAGGCCGCGCTGGCCCTG
GCCGGCCTGACCGCTCGCGTCGTTGACGTCGAGGCCCATGCCCTGGAGCGATCCTTCGGGTTGCTGGCCAATCAATCGGT
GGTGCCCAAGGAGCGCCTGGTGGCGGTGGTCGACATCGGGGCGACCATGACCACCCTGAGCGTGTTGCACCACGGCCGCA
TCATTTATACCCGTGAACAATTGTTCGGTGGCCGTCAACTGACGGAGGACATTCAGCGCCGCTACGGCCTGACCCTTGAA
CAGGCGGGGCTGGCAAAGAAGCAGGGTGGTTTGCCGGATGATTACGTCAACGAAGTGTTGCAGCCGTTTCGCGAGGCGCT
GGTGCAGCAGGTAGCTCGCTCATTGCAGTTTTTCTTCGCTGGCGGCCAATACGATGCTGTCGATCATATCCTGCTGGTCG
GCGGCACCGCCTCGATCTGCGGGCTGGATCGGTTGATCCAGGAGCATTTGGGAACACCGACTCAGGTGGCCAATCCGTTT
GTCGAGATGACGTTGAGCGCCAAGGTCAACGCCGCAGCCCTGGCAAGCGATGCTCCGGCCATGATGATTGCCTGCGGGCT
GGCTCTCAGGAGTTTCGATTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

53.714

99.15

0.533
  comM Acinetobacter nosocomialis M2

53.429

99.15

0.53
  comM Acinetobacter baylyi ADP1

52.408

100

0.524
  pilM Legionella pneumophila strain ERS1305867

45.787

100

0.462

Multiple sequence alignment