Detailed information NTG1107060

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACLKM2_RS11895
• Genome accession: NZ_CP183899
• Coordinates: 2500828..2501886 (-)
• Length: 352 a.a.
• NCBI ID: WP_005168888.1
• Uniprot ID: N9KWF8
• Organism: Acinetobacter pseudolwoffii strain M8_NDM_tet(X3)
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2495828..2506886

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLKM2_RS11870	aroK	2496198..2496734 (-)	537	WP_100355174.1	shikimate kinase AroK	-
ACLKM2_RS11875	pilQ	2496771..2498936 (-)	2166	WP_286412291.1	type IV pilus secretin PilQ family protein	Machinery gene
ACLKM2_RS11880	pilP	2498953..2499480 (-)	528	WP_005094322.1	pilus assembly protein PilP	Machinery gene
ACLKM2_RS11885	comO	2499480..2500193 (-)	714	WP_005094323.1	type 4a pilus biogenesis protein PilO	Machinery gene
ACLKM2_RS11890	comN	2500190..2500828 (-)	639	WP_005094324.1	PilN domain-containing protein	Machinery gene
ACLKM2_RS11895	pilM	2500828..2501886 (-)	1059	WP_005168888.1	pilus assembly protein PilM	Machinery gene
ACLKM2_RS11900	ponA	2502052..2504580 (+)	2529	WP_411686328.1	penicillin-binding protein PBP1a	-
ACLKM2_RS11905	-	2504644..2505447 (-)	804	WP_286411321.1	putative RNA methyltransferase	-
ACLKM2_RS11910	mraY	2505502..2506620 (-)	1119	WP_005094334.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39045.62 Da
• Isoelectric Point: 4.6364

>NTDB_id=1107060 ACLKM2_RS11895 WP_005168888.1 2500828..2501886(-) (pilM) [Acinetobacter pseudolwoffii strain M8_NDM_tet(X3)]
MRRLYRKPNKGLIGVDISSTSVKVLELSVKSSRYWVESYALVPLMENSVVEKNVLNPEAVADALGRAINLANTQSNHAAF
AIPTSMVITKTIEMDADMSNDEREVQIREDAEQYIPFPLDEASLDFEVLPDRLANPNRVNVLLVATRIENIEARSEVLEI
AGLTPKIADVESFALENAFKVFSDTLPMGVNTVGILDIGHSMTTLSVMQNNKIIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKTRALPDDYDIEVLEPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQIDIKKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=1107060 ACLKM2_RS11895 WP_005168888.1 2500828..2501886(-) (pilM) [Acinetobacter pseudolwoffii strain M8_NDM_tet(X3)]
GTGCGCAGGTTATATCGTAAACCAAATAAGGGGTTAATAGGAGTTGATATTAGTTCGACTTCTGTTAAGGTTTTGGAGCT
TTCTGTAAAAAGTAGCCGTTACTGGGTAGAGAGCTATGCTTTGGTCCCATTAATGGAAAACAGTGTTGTTGAAAAAAACG
TCTTAAACCCGGAAGCAGTCGCGGATGCCCTTGGGCGTGCAATTAATTTGGCCAACACCCAGTCGAATCATGCCGCTTTT
GCCATTCCAACCTCTATGGTGATTACCAAAACTATCGAGATGGATGCAGACATGTCGAATGATGAGCGTGAAGTTCAGAT
TCGTGAGGATGCAGAACAGTATATTCCGTTCCCACTCGATGAAGCCAGTCTGGACTTTGAAGTTCTGCCTGACCGTCTTG
CCAATCCAAACCGGGTCAATGTGCTGTTGGTGGCAACCCGCATTGAAAATATTGAAGCACGTTCTGAAGTTTTGGAAATT
GCCGGACTGACACCCAAGATCGCGGATGTAGAAAGTTTTGCGCTGGAAAATGCTTTTAAAGTGTTTTCGGATACTTTGCC
GATGGGGGTAAATACTGTCGGTATTTTAGACATTGGTCATAGTATGACCACCTTGTCTGTCATGCAGAACAACAAAATTA
TTTATACCCGTGAGCAGGTGTTCGGTGGCAAACAGCTGACTCAGGAAATTCAGAACCGTTATGGTCTGTCTTTTGAAGAA
GCCGGGCGTGCTAAAAAAACCCGTGCTTTGCCAGATGACTATGATATTGAAGTACTAGAACCGTTCCTGGATGCCGTGGT
GCAACAGGCAGCGCGGTCATTACAATTCTTCTTTTCATCGTCTCAGTTTAATGAGATTGATCATATTTTATTGGCCGGCG
GGAATGCCAATATTCCAGGTCTGGCAAAATTATTACAACAAAAACTCGGTTACCGTGTCACGATCGCGAATCCATTTTTG
CAAATGGGCTTTTCTCCTCAAATTGATATTAAAAAAATTGAAAATGACGCGTCATCACTCATGGTTGCATGTGGTTTGGC
ATTGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9KWF8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	85.511	100	0.855
comM	Acinetobacter nosocomialis M2	84.943	100	0.849
comM	Acinetobacter baylyi ADP1	79.545	100	0.795
pilM	Legionella pneumophila strain ERS1305867	39.776	100	0.403