Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACM62G_RS07445 Genome accession   NZ_CP183808
Coordinates   1612544..1614244 (+) Length   566 a.a.
NCBI ID   WP_054058987.1    Uniprot ID   -
Organism   Pseudomonas sp. COR18     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1596010..1611802 1612544..1614244 flank 742


Gene organization within MGE regions


Location: 1596010..1614244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM62G_RS07395 (ACM62G_07395) - 1598015..1599547 (+) 1533 WP_419736945.1 NACHT domain-containing protein -
  ACM62G_RS07400 (ACM62G_07400) - 1603099..1603980 (-) 882 WP_419736946.1 hypothetical protein -
  ACM62G_RS07405 (ACM62G_07405) - 1604069..1605481 (-) 1413 Protein_1463 DUF927 domain-containing protein -
  ACM62G_RS07410 (ACM62G_07410) - 1605559..1605828 (-) 270 WP_375135886.1 helix-turn-helix transcriptional regulator -
  ACM62G_RS07415 (ACM62G_07415) - 1605875..1606831 (-) 957 WP_419736947.1 hypothetical protein -
  ACM62G_RS07420 (ACM62G_07420) - 1607122..1608417 (-) 1296 WP_419736948.1 tyrosine-type recombinase/integrase -
  ACM62G_RS07430 (ACM62G_07430) - 1608694..1609752 (-) 1059 WP_419736949.1 O-antigen ligase family protein -
  ACM62G_RS07435 (ACM62G_07435) - 1610156..1611799 (-) 1644 WP_419736950.1 hypothetical protein -
  ACM62G_RS07440 (ACM62G_07440) pilA2 1611921..1612319 (-) 399 WP_419736951.1 pilin Machinery gene
  ACM62G_RS07445 (ACM62G_07445) pilB 1612544..1614244 (+) 1701 WP_054058987.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62633.74 Da        Isoelectric Point: 5.7466

>NTDB_id=1106214 ACM62G_RS07445 WP_054058987.1 1612544..1614244(+) (pilB) [Pseudomonas sp. COR18]
MNDIALSGLAKQLVAAELITDKNAQQAYQQAQRNRMSLVSYLVQNKLVKSREVAEIASEHFGVALLDLNGLDKESQPKGL
VSEKLIRQHHVLPLWRRGNKLFVGISDPSNHQAITDIQFSTGLNTEAILVEDDKLTDAIDKFFDSGATGLEDMADVDLDG
LDIESIDDKKNDPIAGQDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKVYRVRLRTDGMLREVARPPIHLAGRIAARL
KVMANLDISERRKPQDGRIKMRISKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEPDQKDLYMTALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAETLTRLQNMGIPGFNIATSVSLIIAQRLARKLCPHCKREIDIPQETLLKEGFPA
DQIGRFKVYEPVGCDQCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSIEIAEQMRKEGFNDLRTSGLRKAMQGLTSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1106214 ACM62G_RS07445 WP_054058987.1 1612544..1614244(+) (pilB) [Pseudomonas sp. COR18]
ATGAACGATATCGCCCTCTCCGGCCTGGCCAAGCAATTGGTGGCGGCCGAGCTGATTACCGACAAGAACGCTCAGCAGGC
GTATCAGCAAGCCCAACGCAATCGGATGTCCCTGGTCAGCTACCTGGTGCAGAACAAGCTGGTGAAAAGCCGCGAGGTAG
CGGAGATTGCCTCCGAGCACTTCGGCGTCGCCCTGCTCGACCTGAACGGCCTCGACAAGGAAAGCCAGCCCAAGGGCCTG
GTCAGCGAGAAGCTGATCCGCCAGCACCATGTCCTGCCGCTATGGCGGCGCGGCAACAAGCTGTTCGTCGGCATCTCCGA
CCCGAGCAACCACCAGGCGATCACCGATATCCAGTTCAGCACCGGCCTCAATACCGAGGCGATCCTGGTGGAGGACGACA
AGCTCACCGACGCCATCGACAAGTTTTTCGACAGCGGGGCCACCGGTCTCGAAGACATGGCCGATGTCGACCTCGATGGC
CTGGACATCGAGTCGATCGATGACAAGAAGAACGACCCGATCGCCGGCCAGGATGCCGACGACGCCCCGGTGGTGCGCTT
CGTCAACAAGATGTTGCTGGATGCGATCAAGGGTGGCTCTTCGGACCTGCACTTCGAACCCTACGAAAAGGTCTACCGCG
TGCGCCTGCGCACCGACGGCATGCTCCGGGAAGTCGCCCGGCCGCCGATTCACCTGGCCGGGCGGATCGCCGCGCGCCTG
AAGGTCATGGCCAACCTGGATATCTCCGAGCGTCGCAAACCCCAGGACGGCCGGATCAAGATGCGCATCTCGAAGACCAA
GGCCATCGATTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTCATGCGGATCCTCGATCCGTCCAGCG
CCCAGATGGGCATCGATGCCCTGGGCTACGAACCGGATCAGAAAGACCTGTACATGACGGCCCTCAAGCAACCGCAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACCGTCTCGCTCTATACTGGCCTGAATATCCTCAACACCGTGGA
TATCAACATTTCCACTGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAACCCGAAACAGG
GCCTGGACTTCGCCCAGGCCCTGCGCTCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGTGACCTG
GAAACCGCCGAAATCGCGATCAAGGCGGCCCAGACCGGCCACATGGTGCTGTCGACCCTGCACACCAACAGCGCCGCGGA
AACCCTGACCCGCCTGCAGAACATGGGTATCCCCGGGTTCAACATCGCCACCTCGGTCAGCCTGATCATCGCCCAGCGCC
TGGCACGCAAACTGTGCCCGCACTGCAAGAGGGAAATCGATATTCCCCAGGAAACACTCCTTAAGGAAGGTTTCCCTGCG
GATCAGATCGGCAGGTTCAAGGTCTATGAACCTGTGGGCTGCGACCAGTGCAATGGCGGCTACAAGGGGCGCGTGGGAAT
CTACGAGGTGGTGAAGAACACCCCCGACCTGCAACGACTGATCATGGCCGAAGGCAACTCGATCGAGATCGCCGAACAGA
TGCGCAAGGAAGGCTTCAACGACCTGCGGACCTCGGGCCTGCGCAAGGCCATGCAGGGGCTGACCAGCCTGGAAGAAGTA
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.554

100

0.587
  pilB Acinetobacter baylyi ADP1

57.544

100

0.58
  pilB Legionella pneumophila strain ERS1305867

56.184

100

0.562
  pilB Vibrio cholerae strain A1552

51.241

99.647

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

50.088

99.823

0.5
  pilB Vibrio campbellii strain DS40M4

48.785

100

0.496
  pilF Neisseria gonorrhoeae MS11

49.556

99.47

0.493
  pilB/pilB1 Synechocystis sp. PCC 6803

35.275

100

0.385
  pilF Thermus thermophilus HB27

37.455

98.587

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.636

93.286

0.36

Multiple sequence alignment